The effect of osteopontin and osteopontin-derived peptides on preterm brain injury

Background: Osteopontin (OPN) is a highly phosphorylated sialoprotein and a soluble cytokine that is widely expressed in a variety of tissues, including the brain. OPN and OPN-derived peptides have been suggested to have potential neuroprotective effects against ischemic brain injury, but their role in preterm brain injury is unknown. Methods: We used a hypoxia-ischemia (HI)-induced preterm brain injury model in postnatal day 5 mice. OPN and OPN-derived peptides were given intracerebroventricularly and intranasally before HI. Brain injury was evaluated at 7 days after the insults. Results: There was a significant increase in endogenous OPN mRNA and OPN protein in the mouse brain after the induction of HI at postnatal day 5. Administration of full-length OPN protein and thrombin-cleaved OPN did not affect preterm brain injury. This was demonstrated with both intracerebroventricular and intranasal administration of OPN as well as in OPN-deficient mice. Interestingly, both N134–153 and C154–198 OPN-derived peptides increased the severity of brain injury in this HI-induced preterm brain injury model. Conclusions: The neuroprotective effects of OPN are age-dependent, and, in contrast to the more mature brain, OPN-derived peptides potentiate injury in postnatal day 5 mice. Intranasal administration is an efficient way of delivering drugs to the central nervous system (CNS) in neonatal mice and is likely to be an easy and noninvasive method of drug delivery to the CNS in preterm infants

The immune response after hypoxia-ischemia in a mouse model of preterm brain injury

Background: Preterm brain injury consists primarily of periventricular leukomalacia accompanied by elements of gray-matter injury, and these injuries are associated with cerebral palsy and cognitive impairments. Inflammation is believed to be an important contributing factor to these injuries. The aim of this study was to examine the immune response in a postnatal day (PND) 5 mouse model of preterm brain injury induced by hypoxia-ischemia (HI) that is characterized by focal white and gray-matter injury. Methods: C57Bl/6 mice at PND 5 were subjected to unilateral HI induced by left carotid artery ligation and subsequent exposure to 10% O2 for 50 minutes, 70 minutes, or 80 minutes. At seven days post-HI, the white/gray-matter injury was examined. The immune responses in the brain after HI were examined at different time points after HI using RT-PCR and immunohistochemical staining. Results: HI for 70 minutes in PND 5 mice induced local white-matter injury with focal cortical injury and hippocampal atrophy, features that are similar to those seen in preterm brain injury in human infants. HI for 50 minutes resulted in a small percentage of animals being injured, and HI for 80 minutes produced extensive infarction in multiple brain areas. Various immune responses, including changes in transcription factors and cytokines that are associated with a T-helper (Th)1/Th17-type response, an increased number of CD4+ T-cells, and elevated levels of triggering receptor expressed on myeloid cells 2 (TREM-2) and its adaptor protein DNAX activation protein of 12 kDa (DAP12) were observed using the HI 70 minute preterm brain injury model. Conclusions: We have established a reproducible model of HI in PND 5 mice that produces consistent local white/gray-matter brain damage that is relevant to preterm brain injury in human infants. This model provides a useful tool for studying preterm brain injury. Both innate and adaptive immune responses are observed after HI, and these show a strong pro-inflammatory Th1/Th17-type bias. Such findings provide a critical foundation for future studies on the mechanism of preterm brain injury and suggest that blocking the Th1/Th17-type immune response might provide neuroprotection after preterm brain injury

Dynamics of wetlands and their effects on carbon emissions in China coastal region - Case study in Bohai Economic Rim

Wetlands are one of the largest carbon sinks in the world due to their large carbon storage, potential for carbon sequestration in peat formation, sediment deposition and plant biomass. However, rapid economic development is causing changes to wetland carbon storage. China has participated in the implementation of the Kyoto Protocol and is decreasing its carbon emissions. Analyzing the carbon changes that are caused by wetland dynamics may provide some insights regarding decreasing carbon emissions. Therefore, wetland data from 1985, 1995 and 2005 were extracted from remote sensing images. Using spatial analysis and statistics, we determined that the water body area continued to increase, whereas the swamp, floodplain and shallow areas tended to decrease during the period from 1985 to 2005. Those changes caused wetland carbon stock to decrease. The conversion of other land use categories to wetland was the primary cause of carbon stock loss. Therefore, it is more beneficial for China to decrease per capita carbon emissions by decreasing carbon emissions from the conversion of other land use categories to wetlands. (C) 2013 Elsevier Ltd. All rights reserved.Wetlands are one of the largest carbon sinks in the world due to their large carbon storage, potential for carbon sequestration in peat formation, sediment deposition and plant biomass. However, rapid economic development is causing changes to wetland carbon storage. China has participated in the implementation of the Kyoto Protocol and is decreasing its carbon emissions. Analyzing the carbon changes that are caused by wetland dynamics may provide some insights regarding decreasing carbon emissions. Therefore, wetland data from 1985, 1995 and 2005 were extracted from remote sensing images. Using spatial analysis and statistics, we determined that the water body area continued to increase, whereas the swamp, floodplain and shallow areas tended to decrease during the period from 1985 to 2005. Those changes caused wetland carbon stock to decrease. The conversion of other land use categories to wetland was the primary cause of carbon stock loss. Therefore, it is more beneficial for China to decrease per capita carbon emissions by decreasing carbon emissions from the conversion of other land use categories to wetlands. (C) 2013 Elsevier Ltd. All rights reserved

Succession in soil and vegetation caused by coastal embankment in southern Laizhou Bay, China-Flourish or degradation?

Coastal wetland degradation and loss caused by intensive human activities, such as land reclamation and embankment, is a serious environmental problem. In this paper, we studied the succession of soil and vegetation in a coastal wetland dominated by Chinese tamarisk (Tamarix chinensis) community after the construction of a seawall. When the intertidal wetland became supratidal wetland, soil salinity firstly decreased and the gradient from sea to land became weaker. Next, the area of vegetation and plant species diversity increased, and the phenomenon of plant zonation disappeared. We reasoned that the rooted cause of soil and vegetation succession was the change in hydrological environment. Moreover, a Drivers-Pressures-State-Impacts-Response (DPSIR) model was used to analyze the relationship between human activities and coastal wetland. Using this model, we presented the potential problems that might arise in this wetland and predicted that vegetation flourishing was only a short term phenomenon but wetland degradation was the final consequences due to excessive exploitation of groundwater. At last, we proposed a few of operable measures as responses to these potential problems from the perspective of coastal management. (C) 2013 Elsevier Ltd. All rights reserved

Emilin1 gene and essential hypertension: a two-stage association study in northern Han Chinese population

<p>Abstract</p> <p>Background</p> <p>Elastogenesis of elastic extracellular matrix (ECM) which was recognized as a major component of blood vessels has been believed for a long time to play only a passive role in the dynamic vascular changes of typical hypertension. Emilin1 gene participated in the transcription of ECM's formation and was recognized to modulate links TGF-β maturation to blood pressure homeostasis in animal study. Recently relevant advances urge further researches to investigate the role of Emilin1 gene in regulating TGF-β signals involved in elastogenesis and vascular cell defects of essential hypertension (EH).</p> <p>Methods</p> <p>We designed a two-stage case-control study and selected three single nucleotide polymorphisms (SNPs), rs3754734, rs2011616 and rs2304682 from the HapMap database, which covered Emilin1 gene. Totally 2,586 subjects were recruited from the International Collaborative Study of Cardiovascular Disease in Asia (InterASIA). In stage 1, all the three SNPs of the Emilin1 gene were genotyped and tested within a subsample including 503 cases and 490 controls, significant SNPs would enter into stage 2 including 814 cases with hypertension and 779 controls and analyze on the basis of testing total 2,586 subjects.</p> <p>Results</p> <p>In stage 1, single locus analyses showed that SNPs rs3754734 and rs2011616 had significant association with EH (P < 0.05). In stage 2, weak association for dominant model were observed by age stratification and odds ratio (ORs) of TG+GG vs. TT of rs3754734 were 0.768 (0.584-1.009), 0.985 (0.735-1.320) and 1.346 (1.003-1.806) in < 50, 50-59 and ≥ 60 years group and ORs of GA+AA vs. GG of rs2011616 were 0.745 (0.568-0.977), 1.013 (0.758-1.353) and 1.437 (1.072-1.926) in < 50, 50-59 and ≥ 60 years group respectively. Accordingly, significant interactions were detected between genotypes of rs3754734 and rs2011616 and age for EH, and ORs were 1.758 (1.180-2.620), P = 0.006 and 1.903 (1.281-2.825), P = 0.001, respectively. Results of haplotypes analysis showed that there weren't any haplotypes associated with EH directly, but the interaction of hap2 (GA) and age-group found to be significant after being adjusted for the covariates, OR was 1.220 (1.031-1.444), P value was 0.020.</p> <p>Conclusion</p> <p>Our findings don't support positive association of Emilin1 gene with EH, but the interaction of age and genotype variation of rs3754734 and rs2011616 might increase the risk to hypertension.</p

Statistical investigation of a dehumidification system performance using Gaussian process regression

Swift performance assessment of dehumidification systems, in design stage and while operation of the system is of substantial importance for commercialization and wide implementation of this technology. This paper presents a novel statistical model, employing Gaussian Process Regression (GPR) to investigate performance of a solar/waste energy driven dehumidification/regeneration cycle with a solid adsorbent bed. The statistical model takes thousands of operating conditions derived from a numerical model to predict the performance of the system. This predictive tool directly correlates the main operating parameters with the performance parameters of the system. The operating parameters considered in this study are: temperature, relative humidity and flow rate of process air, temperature of regeneration air, length of the desiccant bed, solar radiation intensity and operating time, and the selected performance parameters are: moisture extraction efficiency for the dehumidification cycle and moisture removal efficiency for the regeneration cycle. The model is evaluated by three metrics, namely: root mean square error (RSME), mean absolute percentage error (MAPE), and coefficient of determination (R2). The maximum RSME and MAPE for moisture extraction are only 0.045, 0.21%, and for moisture removal efficiencies are 0.082 and 0.39%, respectively, while the R2 value is derived as 0.97. The developed model is used to investigate the impact of four selected operating parameters on system performance. Additionally, the system performance is predicted for randomly generated operating conditions as well as warm and humid climates. The developed GPR model provides a swift and highly accurate predictive tool for design of the dehumidification systems and for commercialization of the investigated dehumidification systems

The large area detector onboard the eXTP mission

The Large Area Detector (LAD) is the high-throughput, spectral-timing instrument onboard the eXTP mission, a flagship mission of the Chinese Academy of Sciences and the China National Space Administration, with a large European participation coordinated by Italy and Spain. The eXTP mission is currently performing its phase B study, with a target launch at the end-2027. The eXTP scientific payload includes four instruments (SFA, PFA, LAD and WFM) offering unprecedented simultaneous wide-band X-ray timing and polarimetry sensitivity. The LAD instrument is based on the design originally proposed for the LOFT mission. It envisages a deployed 3.2 m2 effective area in the 2-30 keV energy range, achieved through the technology of the large-area Silicon Drift Detectors - offering a spectral resolution of up to 200 eV FWHM at 6 keV - and of capillary plate collimators - limiting the field of view to about 1 degree. In this paper we will provide an overview of the LAD instrument design, its current status of development and anticipated performance