Comparative expression profiles of carboxylesterase orthologous CXE14 in two closely related tea geometrid species, Ectropis obliqua Prout and Ectropis grisescens Warren

Insect carboxylesterases (CXEs) can be expressed in multiple tissues and play crucial roles in detoxifying xenobiotic insecticides and degrading olfactory cues. Therefore, they have been considered as an important target for development of eco-friendly insect pest management strategies. Despite extensive investigation in most insect species, limited information on CXEs in sibling moth species is currently available. The Ectropis obliqua Prout and Ectropis grisescens Warren are two closely related tea geometrid species, which share the same host of tea plant but differ in geographical distribution, sex pheromone composition, and symbiotic bacteria abundance, providing an excellent mode species for studies of functional diversity of orthologous CXEs. In this study, we focused on EoblCXE14 due to its previously reported non-chemosensory organs-biased expression. First, the EoblCXE14 orthologous gene EgriCXE14 was cloned and sequence characteristics analysis showed that they share a conserved motif and phylogenetic relationship. Quantitative real-time polymerase chain reaction (qRT-PCR) was then used to compare the expression profiles between two Ectropis spp. The results showed that EoblCXE14 was predominately expressed in E. obliqua larvae, whereas EgriCXE14 was abundant in E. grisescens at multiple developmental stages. Interestingly, both orthologous CXEs were highly expressed in larval midgut, but the expression level of EoblCXE14 in E. obliqua midgut was significantly higher than that of EgriCXE14 in E. grisescens midgut. In addition, the potential effect of symbiotic bacteria Wolbachia on the CXE14 was examined. This study is the first to provide comparative expression profiles of orthologous CXE genes in two sibling geometrid moth species and the results will help further elucidate CXEs functions and identify a potential target for tea geometrid pest control

The mitochondrial genome of Ectropis dentilineata Moore, 1868 (Lepidoptera: Geometridae) from tea plantations in Guizhou province, China

Ectropis dentilineata Moore, 1868 has a wide geographic distribution while its genome data are largely unknown. In this study, we collected samples of E. dentilineata from a tea plantation in Guizhou province, China. Illumina sequencing showed that the mitochondrial genome of E. dentilineata is 15,356 bp in length, containing the entire set of 37 mitochondrial genes. Several tRNAs show gene arrangements compared with the ancestral gene order, mainly involving the tRNA cluster (M-I-Q). These data will facilitate a deeper understanding and exploitation of E. dentilineata

The Mitochondrial Genomes of Two Parasitoid Wasps <i>Protapanteles immunis</i> and <i>Parapanteles hyposidrae</i> (Hymenoptera: Braconidae) with Phylogenetic Implications and Novel Gene Rearrangements

Parapanteles hypsidrae (Wilkinson, 1928) and Protapanteles immunis (Haliday, 1834) are the most important parasitic wasps of Ectropis grisescens Warren and Ectropis obliqua (Prout). We sequenced and annotated the mitochondrial genomes of Pa. hyposidrae and Pr. immunis, which are 17,063 bp and 16,397 bp in length, respectively, and possess 37 mitochondrial genes. We discovered two novel types of gene rearrangement, the local inversion of nad4L in Pa. hyposidrae and the remote inversion of the block cox3-nad3-nad5-nad4 in Pr. immunis, within the mitogenomes of Braconidae. The phylogenetic analysis supported the subfamily Microgastrinae is a monophyletic group, but the tribes Apantelini and Cotesiini within this subfamily are paraphyletic groups

An Evaluation Method of Brittleness Characteristics of Shale Based on the Unloading Experiment

Shale reservoir has an initial unloading effect during the natural uplift and erosion process, which causes the shale brittleness to change, affecting the design of the fracturing scheme. To consider this, the axial compression loading and confining pressure unloading experiment of shale is carried out, and then the influence of unloading rate on the mechanical parameters, failure characteristics, and the brittleness of rock are analyzed. What is more, a new evaluation method of brittleness characteristics that take the unloading effect into consideration is proposed. The conclusions are as follows: (1) The unloading rate has a weakening effect on the mechanical parameters, such as the destructive confining pressure and the residual strength of the samples. (2) The failure characteristics of shale specimens are a single shear failure in an oblique section under low unloading rate, and multiple shear zones accompanied with bedding fracture under high unloading rate. (3) The brittleness of shale samples is well verified by the brittleness index B d 1 and B d 2 during the loading path; nevertheless, it has shortage at the unloading path. This paper proposes a new brittleness evaluation method which can consider the influence of the different unloading rates and unloading points. Furthermore, there is a nice characterization between the brittleness damage and this method

Expression of Key Structural Genes of the Phenylpropanoid Pathway Associated with Catechin Epimerization in Tea Cultivars

Catechin epimerization is an important factor affecting tea catechin compositions and thereby tea quality. However, a lack of tea germplasms with high non-epicatechins limits relative research. Here, a tea cultivar Y510 with high non-epicatechins was firstly reported and used for catechin and RNA sequencing (RNA-Seq) analysis. Results showed that the (-)-gallocatechin gallate and (+)-catechin (C) contents in Y510 were at least 136 and 6 times higher than those in Fudingdabaicha and 0306I, but the epicatechins (-)-epigallocatechin and (-)-epicatechin (EC) were significantly lower. Eleven unigenes potentially involved in catechin epimerization were identified by RNA-Seq analysis. Based on a combination of catechin and gene expression analysis, it was hypothesized that two anthocyanidin reductase genes (CsANR1, CsANR2) and an anthocyanidin synthase gene (CsANS) are the key genes affecting catechin epimerization in tea. Non-epicatechin formations were hypothesized to be mainly influenced by the expression ratio of CsANR2 to CsANR1 and the expression of CsANS. Overexpression of CsANS in an Arabidopsis mutant tds4-2 led to a significant increase of EC accumulation in seeds, revealing CsANS is important for catechin epimerization. These results shed new light on breeding tea cultivars with special catechin compositions

Application technology of the sex pheromone of the tea geometrid <i>Ectropis grisescens</i> (Lepidoptera: Geometridae)

<p>The sex pheromone of <i>Ectropis grisescens</i>, one of the primary defoliator insects of tea plantations, has been identified, but its trapping parameters have not been optimized for field application. In this study, we investigated the effects of pheromone dose, trap height and trap type on the effectiveness of trapping <i>E. grisescens</i> in the field. Our results show that the optimal pheromone dose is 800 μg. The bucket-funnel trap hung 40 cm below the tea canopy had the highest trapping efficiency. This study establishes optimized parameters for the pheromone trapping of <i>E. grisescens</i> in tea plantations.</p