Contemporary Squares of China--Nanjing Case Study

In urban history books about Chinese cities, all the vivid descriptions of traditional urban life convey one message--as an important part of the city today, square seems to be absence in China’s past. Square was imported to China along with the establishment of concessions. When foreigners (Russian/German/Italian/, etc.) introduce square toChina along with constructing their concessions, they would never image that Chinese square will become something highly peculiar and unusual in many ways after 100 years. However, the form and function of Chinese square appear quite similar with European square in the very beginning, so what forms the peculiar characters of Chinese today? The answer to this question may start from China’s failure of ‘the first Sino-Japanese war, since then The foundation of Chinese ideology and philosophy--Confucianism was doubted and questioned. Meanwhile, Social Darwinism was influential and prevailed nationwide. the direct influence of these 2 transformations are western culture was considered as higher potential culture. This idea leading to the disappear of traditional public spaces like streets and appear of western public spaces like squares or parks. With the purpose to reform the public life and remake the public space, there are 3 stages for square design in China.All these ideological transformations and urban reform have 3 points influence square design of China, the first is the disappear of China's traditional street culture, the second is the residue of collectivism since Mao's era, the third is the influence of the Soviet Union and Beaux-Arts system. In this context, there are similar and peculiar characters in both form and function. In this study, Nanjing was selected as studying subject. Nanjing was the capital of China on many occasions. The name Nanjing literally means ‘the southern capital’ and its counterpart is Beijing—means ‘the northern capital’. Among them, the urban design of Ming Empire and Republic China contributes greatly to influence the urban structure of Nanjing today. All the five cases selected for study have close relationship with traces of republic china, 3 of them have relationship with remains of Ming Empire

A polymer gel index-matched to water enables diverse applications in fluorescence microscopy [preprint]

We demonstrate diffraction-limited and super-resolution imaging through thick layers (tens-hundreds of microns) of BIO-133, a biocompatible, UV-curable, commercially available polymer with a refractive index (RI) matched to water. We show that cells can be directly grown on BIO-133 substrates without the need for surface passivation and use this capability to perform extended time-lapse volumetric imaging of cellular dynamics 1) at isotropic resolution using dual-view light-sheet microscopy, and 2) at super-resolution using instant structured illumination microscopy. BIO-133 also enables immobilization of 1) Drosophila tissue, allowing us to track membrane puncta in pioneer neurons, and 2) Caenorhabditis elegans, which allows us to image and inspect fine neural structure and to track pan-neuronal calcium activity over hundreds of volumes. Finally, BIO-133 is compatible with other microfluidic materials, enabling optical and chemical perturbation of immobilized samples, as we demonstrate by performing drug and optogenetic stimulation on cells and C. elegans

Projected Spatiotemporal Dynamics of Drought under Global Warming in Central Asia

Drought, one of the most common natural disasters that have the greatest impact on human social life, has been extremely challenging to accurately assess and predict. With global warming, it has become more important to make accurate drought predictions and assessments. In this study, based on climate model data provided by the Inter-Sectoral Impact Model Intercomparison Project (ISIMIP), we used the Palmer Drought Severity Index (PDSI) to analyze and project drought characteristics and their trends under two global warming scenarios—1.5 °C and 2.0 °C—in Central Asia. The results showed a marked decline in the PDSI in Central Asia under the influence of global warming, indicating that the drought situation in Central Asia would further worsen under both warming scenarios. Under the 1.5 °C warming scenario, the PDSI in Central Asia decreased first and then increased, and the change time was around 2080, while the PDSI values showed a continuous decline after 2025 in the 2.0 °C warming scenario. Under the two warming scenarios, the spatial characteristics of dry and wet areas in Central Asia are projected to change significantly in the future. In the 1.5 °C warming scenario, the frequency of drought and the proportion of arid areas in Central Asia were significantly higher than those under the 2.0 °C warming scenario. Using the Thornthwaite (TH) formula to calculate the PDSI produced an overestimation of drought, and the Penman–Monteith (PM) formula is therefore recommended to calculate the index

The influence of uncertain expectations on Chinese rural residents’ consumer behavior decisions: Theoretical analysis and empirical test

Economic uncertainty will result in consumers’ unstable environment expectations, thus affecting household consumption decisions. The paper established a consumption model though using the cointegration theory and ECM model, and used the annual data from 2002 to 2021 to examine the influence of uncertain expectations on the consumption behavior of rural residents in China. The research results show that consumers’ income, consumption, consumption habits liquidity constraints and precautionary savings jointly produce the feelings of uncertainty, which will lead to rural consumers’ uncertain expectations. Uncertain expectations will not only lead to rural residents the prudent consumption behavior of residents, but also lead to the lack of confidence of residents, forming phased consumption characteristics. Combining the results of the empirical analysis, this paper gives suggestions to promote rural consumption from the perspective of increasing rural residents’ income, reforming the income distribution system, improving the rural financial system, and reinforcing the construction of rural consumption infrastructure

Phosphorylation of TGB1 by protein kinase CK2 promotes barley stripe mosaic virus movement in monocots and dicots.

The barley stripe mosaic virus (BSMV) triple gene block 1 (TGB1) protein is required for virus cell-to-cell movement. However, little information is available about how these activities are regulated by post-translational modifications. In this study, we showed that the BSMV Xinjiang strain TGB1 (XJTGB1) is phosphorylated in vivo and in vitro by protein kinase CK2 from barley and Nicotiana benthamiana. Liquid chromatography tandem mass spectrometry analysis and in vitro phosphorylation assays demonstrated that Thr-401 is the major phosphorylation site of the XJTGB1 protein, and suggested that a Thr-395 kinase docking site supports Thr-401 phosphorylation. Substitution of Thr-395 with alanine (T395A) only moderately impaired virus cell-to-cell movement and systemic infection. In contrast, the Thr-401 alanine (T401A) virus mutant was unable to systemically infect N. benthamiana but had only minor effects in monocot hosts. Substitution of Thr-395 or Thr-401 with aspartic acid interfered with monocot and dicot cell-to-cell movement and the plants failed to develop systemic infections. However, virus derivatives with single glutamic acid substitutions at Thr-395 and Thr-401 developed nearly normal systemic infections in the monocot hosts but were unable to infect N. benthamiana systemically, and none of the double mutants was able to infect dicot and monocot hosts. The mutant XJTGB1T395A/T401A weakened in vitro interactions between XJTGB1 and XJTGB3 proteins but had little effect on XJTGB1 RNA-binding ability. Taken together, our results support a critical role of CK2 phosphorylation in the movement of BSMV in monocots and dicots, and provide new insights into the roles of phosphorylation in TGB protein functions