Additional file 1: of Assessment of hospital length of stay and direct costs of type 2 diabetes in Hubei Province, China

LOS and direct costs of type2 diabetes. Title of data: Sheet1 (LOS and direct costs of all type2 diabetes patients); Sheet2 (LOS and direct costs of type2 diabetes patients with chronic complications). Description of data: Basic information such as type of medical insurance, gender, age, occupation, method of admission, LOS, type of complications and cost information for drugs, accommodation, imaging, therapeutics, laboratory analysis, sanitary materials, diagnostics, and nursing care are included. (XLSX 540 kb

Mitochondrial Membrane Potential Independent Near-Infrared Mitochondrial Viscosity Probes for Real-Time Tracking Mitophagy

Mitophagy is a vital cellular process playing vital roles in regulating cellular metabolism and mitochondrial quality control. Mitochondrial viscosity is a key microenvironmental index, closely associated with mitochondrial status. To monitor mitophagy and mitochondrial viscosity, three molecular rotors (Mito-1, Mito-2, and Mito-3) were developed. All probes contain a cationic quinolinium unit and a C12 chain so that they can tightly bind mitochondria and are not affected by the mitochondrial membrane potential. Optical studies showed that all probes are sensitive to viscosity changes with an off–on fluorescence response, and Mito-3 shows the best fluorescence enhancement. Bioimaging studies showed that all these probes can not only tightly locate and visualize mitochondria with near-infrared fluorescence but also effectively monitor the mitochondrial viscosity changes in cells. Moreover, Mito-3 was successfully applied to visualize the mitophagy process induced by starvation, and mitochondrial viscosity was found to show an increase during mitophagy. We expect Mito-3 to become a useful imaging tool for studying mitochondrial viscosity and mitophagy

Data_Sheet_1_The association of energy and macronutrient intake at breakfast and cardiovascular disease in Chinese adults: From a 14-year follow-up cohort study.docx

BackgroundWe aimed to examine the associations between energy and macronutrient intakes at breakfast and the incidence of cardiovascular events among Chinese adults.MethodsThere were 12,937 participants from the China Health and Nutrition Survey who met the study criteria and completed six rounds of questionnaires in 1997, 2000, 2004, 2006, 2009, and 2011. Combined weighing methods with 24-h dietary recall were used to measure dietary intake throughout the day. Intakes of macronutrients at breakfast were calculated using energy provided by nutrients as a percentage of breakfast energy. We calculated hazard ratios using a multivariable Cox frailty model with random intercepts to account for household clustering.ResultsDuring follow-up, we documented 453 (3.6 per 1,000 person-years) major cardiovascular events, 195 (1.5 per 1,000 person-years) myocardial infarctions, and 293 (2.3 per 1,000 person-years) strokes. In Chinese adults, more breakfast carbohydrates or less proteins intake was associated with the reduced risk of cardiovascular diseases. Especially for women, higher intake of breakfast carbohydrates was associated with a lower risk of major cardiovascular events (quintile 5 vs. quintile 1, HR 0.47 [95%CI 0.30–0.74]; ptrend = 0.0008) and stroke (quintile 5 vs. quintile 1, HR 0.48 [95%CI 0.26–0.88]; ptrend = 0.0006). Higher intake of breakfast proteins was associated with a higher risk of major cardiovascular events (quintile 5 vs. quintile 1, HR 1.77 [95%CI 1.12–2.79]; ptrend = 0.1162), myocardial infarction (quintile 5 vs. quintile 1, HR 2.49 [95%CI 1.21–5.11]; ptrend = 0.2641). There was a significant association between breakfast fat intake and cardiovascular diseases in the adult population, but less significant correlation was found in Chinese men or women. Breakfast fat intake was positively associated with the risk of major cardiovascular events (quintile 5 vs. quintile 1, HR 1.74 [95%CI 1.27–2.36]; ptrend = 0.0070), myocardial infarction (quintile 5 vs. quintile 1, HR 2.03 [95%CI 1.23–3.37]; ptrend = 0.0168), and stroke (quintile 5 vs. quintile 1, HR 1.64 [95%CI 1.12–2.41]; ptrend = 0.0732). There was a significant reduction in major cardiovascular events and stroke when breakfast energy intake was moderated, even if the independence of skipping breakfast.ConclusionHigh carbohydrate intake and low protein and fat intake at breakfast may contribute to cardiovascular health while maintaining a moderate energy intake.</p

Electron Beam Etching of CaO Crystals Observed Atom by Atom

With the rapid development of nanoscale structuring technology, the precision in the etching reaches the sub-10 nm scale today. However, with the ongoing development of nanofabrication the etching mechanisms with atomic precision still have to be understood in detail and improved. Here we observe, atom by atom, how preferential facets form in CaO crystals that are etched by an electron beam in an in situ high-resolution transmission electron microscope (HRTEM). An etching mechanism under electron beam irradiation is observed that is surprisingly similar to chemical etching and results in the formation of nanofacets. The observations also explain the dynamics of surface roughening. Our findings show how electron beam etching technology can be developed to ultimately realize tailoring of the facets of various crystalline materials with atomic precision

Response to novel s in verse in Czech literature

This thesis is focused on response to novel in verse in Czech Literature of 19th century. The form of novel in verse becomes attractive for authors in Czech Literature since the second half of 19th century, especially after familiarizing with Puškin's Evžen Oněgin. In this work we try to cover questions of novel in verse, then to put near responses to Evžen Oněgin in Czech context (picked pieces by J. Neruda, B. Kaminský, F. Táborský, K. Leger, M. A. Šimáček). Subsequently there is more space given to first try of handling the genre - Pan Vyšínský - by Gustav Ptleger Moravský, and supreme realization of novel in verse - Josef Svatopluk Machar's Magdalena

Key nodes of the PPI network visualized by Cytoscape software.

<p>All nodes that have a centrality value greater or equal to the threshold are highlighted with a light gray color in the network view. (A) up-regulated proteins; (B) down-regulated proteins.</p

Differentially proteins play a major role in cell migration, and invasion of PC-1.0 Metastatic.

<p>(A) Effect of three proteins on the migration after using siRNA or antibody blocking in highly metastatic PC-1.0 cells. The cells were incubated for 24h. The percentage migration was calculated and graphed. * Compared with the PC-1.0, <i>P</i>-value <0.01. (n = 3) (B) Quantification of transwell assay for treated group and control group. Cells were counted in triplicate wells and in three identical experiments, * Compared with the PC-1.0, <i>P</i>-value <0.01. (n = 3)</p

Protein-protein interaction network and signaling pathway analysis.

<p>(A) A PPI network, as shown in the interactive view, generated by a STRING database to reveal functional interactions between up-regulated proteins. Each node represents a protein, and each edge represents an interaction (<i>P</i>-values = 6.06E-12); (B) Up-regulated proteins’ MCL cluster algorithms derived using STRING. MCL = 2; (C) Up-regulated proteins’ K-Means cluster algorithms. K-Means = 4; approximate same consequences by two clusters; (D) A PPI network reveals functional interactions between down-regulated proteins (<i>P</i>-value = 1.47E-6); (E) Down-regulated proteins’MCL cluster algorithms. MCL = 2; (F) Down-regulated proteins’ K-Means cluster algorithms. K-Means = 4.</p

Significantly enriched modular of down-regulated proteins using CentiScaPe software.

<p>Significantly enriched modular of down-regulated proteins using CentiScaPe software.</p

Phosphoproteome Analysis of Invasion and Metastasis-Related Factors in Pancreatic Cancer Cells - Fig 3

<p><b>KEGG and BioCarta pathway functional annotations of differentially expressed up-regulated (A, C) and down-regulated (B) proteins (<i>P</i>-values < 0.01, Benjamin < 0.05).</b> Enrichment scores corresponding to each pathway provided by the DAVID annotation tool are displayed as −log <i>P</i>-values. Down-regulated proteins that were BioCarta pathway functional annotations only were enriched in apoptotic signaling in response to DNA damage.</p