Additional file 1: Table S1-S5. of Follicular thyroid carcinoma but not adenoma recruits tumor-associated macrophages by releasing CCL15

Comparison of CD68 immunostain in FTCs/FAs in tissue microarray and in the corresponding entire tissue sample sections. Table S2. Increased Density of CD206 Positive Macrophages in FTCs comparing to FAs. Table S3. Correlations of CD68 Positive TAMs Density with Clinicopathological Features of FTC Patients. Table S4. Correlations of CCL15 expression level with Clinicopathological Features of FTC Patients. Table S5. Sequence of primers used in this study. (DOCX 25.5 kb

Additional file 2: Figure S1 and S2. of Follicular thyroid carcinoma but not adenoma recruits tumor-associated macrophages by releasing CCL15

Immunostain of CD68 in entire tissue of FTC/FA samples. (A) An example of immunohistochemistry analysis of CD68 in whole tissue samples of FTC (left panel) and FA (right panel). Stars indicated blank areas were taked out for tissue microarrays constructing. CD68+ cells in ten 200 μm *300 μm areas (green-red marked) of every sample were counted. Bar = 1 mm. (B) Enlarged picture of one count area (one green-red area in A). Arrows indicate the CD68+ macrophages in FTC (left panel) or FA (right panel). Bar = 20 μm. Figure S2. Densities of CD206+ cells in FTC are significantly higher than those in FA. Immunohistochemistry analysis of CD206 in 55 cases of tissue samples from FTC and FA patients. Arrows indicate the CD206+ macrophages. Bar = 20 μm. (PDF 509 kb

Correlation of ADC values of the whole osteosarcomas with necrosis rates after neoadjuvant chemotherapy.

<p>Correlation of ADC values of the whole osteosarcomas with necrosis rates after neoadjuvant chemotherapy.</p

Osteosarcoma of the distal femur in a 46-year-old woman with poor response.

<p>(<b>A∼C</b>) DWI map, ADC map and ADC histogram before neoadjuvant chemotherapy. The tumor on DWI map was mixed high signal intensity. The ADC value of the whole tumor was 1.35×10⁻³ mm²/s with a subtotal green area. The ADC histogram was high and sharp. (<b>D∼F</b>) DWI map, ADC map and ADC histogram after neoadjuvant chemotherapy. The tumor on DWI map was mixed signal intensity. The ADC value of the whole tumor (1.31×10⁻³ mm²/s) and the ADC histogram were similar to those of pre- neoadjuvant chemotherapy. Tumor necrosis rate of 20% was confirmed by postoperative pathological evaluation. The effectiveness of neoadjuvant chemotherapy was poor.</p

Tumor size and volume at pre- and post-chemotherapy.

<p>Tumor size and volume at pre- and post-chemotherapy.</p

Osteosarcoma of the distal femur in a 24-year-old man with good response.

<p>(<b>A∼C</b>) DWI map, ADC map and ADC histogram before neoadjuvant chemotherapy. The signal intensity of tumor on DWI map was high. The ADC value of the whole tumor was 1.12×10⁻³ mm²/s with a green area. The ADC histogram was high and sharp. (<b>D∼F</b>) DWI map, ADC map and ADC histogram after neoadjuvant chemotherapy. The signal intensity of tumor on DWI map was decreased. The ADC value of the whole tumor was increased to 1.99×10⁻³ mm²/s with a subtotal red area. The ADC histogram was short, wide and moving to the right of the coordinate. Tumor necrosis rate of 92% was confirmed by postoperative pathological evaluation. The effectiveness of neoadjuvant chemotherapy was good.</p

Mean ADC values of whole tumor at pre- and post-chemotherapy (×10⁻³ mm²/s).

<p>Mean ADC values of whole tumor at pre- and post-chemotherapy (×10⁻³ mm²/s).</p