An Improved FPT Algorithm for the Flip Distance Problem

Given a set cal P of points in the Euclidean plane and two triangulations of cal P, the flip distance between these two triangulations is the minimum number of flips required to transform one triangulation into the other. The Parameterized Flip Distance problem is to decide if the flip distance between two given triangulations is equal to a given integer k. The previous best FPT algorithm runs in time O^*(kcdot c^k) (cleq 2times 14^11), where each step has fourteen possible choices, and the length of the action sequence is bounded by 11k. By applying the backtracking strategy and analyzing the underlying property of the flip sequence, each step of our algorithm has only five possible choices. Based on an auxiliary graph G, we prove that the length of the action sequence for our algorithm is bounded by 2|G|. As a result, we present an FPT algorithm running in time O^*(kcdot 32^k)

OP9-Lhx2 stromal cells facilitate derivation of hematopoietic progenitors both in vitro and in vivo

AbstractGenerating engraftable hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is an ideal approach for obtaining induced HSCs for cell therapy. However, the path from PSCs to robustly induced HSCs (iHSCs) in vitro remains elusive. We hypothesize that the modification of hematopoietic niche cells by transcription factors facilitates the derivation of induced HSCs from PSCs. The Lhx2 transcription factor is expressed in fetal liver stromal cells but not in fetal blood cells. Knocking out Lhx2 leads to a fetal hematopoietic defect in a cell non-autonomous role. In this study, we demonstrate that the ectopic expression of Lhx2 in OP9 cells (OP9-Lhx2) accelerates the hematopoietic differentiation of PSCs. OP9-Lhx2 significantly increased the yields of hematopoietic progenitor cells via co-culture with PSCs in vitro. Interestingly, the co-injection of OP9-Lhx2 and PSCs into immune deficient mice also increased the proportion of hematopoietic progenitors via the formation of teratomas. The transplantation of phenotypic HSCs from OP9-Lhx2 teratomas but not from the OP9 control supported a transient repopulating capability. The upregulation of Apln gene by Lhx2 is correlated to the hematopoietic commitment property of OP9-Lhx2. Furthermore, the enforced expression of Apln in OP9 cells significantly increased the hematopoietic differentiation of PSCs. These results indicate that OP9-Lhx2 is a good cell line for regeneration of hematopoietic progenitors both in vitro and in vivo

A Design Method to Diagnose Predicted and Measured Energy Performance Gap of Central Cooling Plant Based on Operation Mode

Operation modes indicate the characteristics of the entire cooling plant, and are considered to be an underlying factor of energy performance gap. Operation mode difference between the simulation model and actual system is constituted by the differences in mode form, mode frequency, and mode energy consumption jointly. In the numerical model of ECDR, the energy performance gap is the function of the components of the operation mode difference. Based on the model, a central cooling plant case is analysed. Five typical mode forms for the simulation model and six for the actual system are extracted. For the corresponding mode form, due to the significant differences in cooling load characteristics and chiller energy efficiency, deviations in mode frequency are up to 55.6%, and the deviations in mode energy consumption ranges from -0.9% to 19.4%

Design and implementation of an automatic charging system for intelligent patrol robot

This paper is concerned with the design and implementation of an automatic charging system for the intelligent patrol robot. An economic and practical method combined with the infrared sensor and laser sensor is developed to realize the accurate automatic charge docking. The phase-shifted full-bridge ZVS-PWM converter is adopted to design an automatic charging pile, which uses a constant voltage limited current charging mode and improve the efficiency of the charging. Several simulations and experiments are implemented to test the automatic charge docking system, and the results could demonstrate the effectiveness and superiority of the system

Characterization and Antibacterial Activities of Carboxymethylated Paramylon from Euglena gracilis

Paramylon from Euglena gracilis (EGP) is a polymeric polysaccharide composed of linear β-1,3 glucan. EGP has been proved to have antibacterial activity, but its effect is weak due to its water insolubility and high crystallinity. In order to change this deficiency, this experiment carried out carboxymethylated modification of EGP. Three carboxymethylated derivatives, C-EGP1, C-EGP2, and C-EGP3, with a degree of substitution (DS) of 0.14, 0.55, and 0.78, respectively, were synthesized by varying reaction conditions, such as the mass of chloroacetic acid and temperature. Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) analysis confirmed the success of the carboxymethylated modification. The Congo red (CR) experiment, scanning electron microscopy (SEM), X-ray diffraction (XRD), and thermogravimetry (TG) were used to study the conformation, surface morphology, crystalline nature, and thermostability of the carboxymethylated EGP. The results showed that carboxymethylation did not change the triple helix structure of the EGP, but that the fundamental particles’ surface morphology was destroyed, and the crystallization area and thermal stability decreased obviously. In addition, the water solubility test and antibacterial experiment showed that the water solubility and antibacterial activity of the EGP after carboxymethylation were obviously improved, and that the water solubility of C-EGP1, C-EGP2, and C-EGP3 increased by 53.31%, 75.52%, and 80.96% respectively. The antibacterial test indicated that C-EGP3 had the best effect on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with minimum inhibitory concentration (MIC) values of 12.50 mg/mL and 6.25 mg/mL. The diameters of the inhibition zone of C-EGP3 on E. coli and S. aureus were 11.24 ± 0.15 mm and 12.05 ± 0.09 mm, and the antibacterial rate increased by 41.33% and 43.67%

Thermal Error Prediction and Compensation of Digital Twin Laser Cutting Based on T-XGBoost

Laser cutting belongs to non-contact processing, which is different from traditional turning and milling. In order to improve the machining accuracy of laser cutting, a thermal error prediction and dynamic compensation strategy for laser cutting is proposed. Based on the time-varying characteristics of the digital twin technology, a hybrid model combining the thermal elastic–plastic finite element (TEP-FEM) and T-XGBoost algorithms is established. The temperature field and thermal deformation under 12 common working conditions are simulated and analyzed with TEP-FEM. Real-time machining data obtained from TEP-FEM simulation is used in intelligent algorithms. Based on the XGBoost algorithm and the simulation data set as the training data set, a time-series-based segmentation algorithm (T-XGBoost) is proposed. This algorithm can reduce the maximum deformation at the slit by more than 45%. At the same time, by reducing the average volume strain under most working conditions, the lifting rate can reach 63% at the highest, and the machining result is obviously better than XGBoost. The strategy resolves the uncontrollable thermal deformation during cutting and provides theoretical solutions to the implementation of the intelligent operation strategies such as predictive machining and quality monitoring

Evaluation and Prediction of COVID-19 Prevention and Control Strategy Based on the SEIR-AQ Infectious Disease Model

Based on the SEIR model, which takes into account prevention and control measures, prevention and control awareness, and economic level and medical level indicators, this paper proposes an infectious disease model of “susceptible-exposed-infected-removed-asymptomatic-isolated” (short for SEIR-AQ) to assess and predict the development of the COVID-19 pandemic with different prevention and control strategies. The kinetic parameters of the SEIR-AQ model were obtained by fitting, and the parameters of the SEIR-AQ model were solved through the Euler method. Furthermore, the effects of different countries’ prevention and control strategies on the number of infections, the proportion of isolation, the number of deaths, and the number of recoveries were also simulated. The theoretical analysis showed that measures such as isolation for prevention and control and medical tracking isolation had a significant inhibitory effect on the development of the COVID-19 pandemic, among which stratified treatment and enhanced awareness played a key role in the rapid regression of the peak of COVID-19-infected patients. Conclusion of the Simulation. The SEIR-AQ model can be used to evaluate the development status of the COVID-19 epidemic and has some theoretical value for the prediction of COVID-19

Potential use of hyperoxygenated solution as a treatment strategy for carbon monoxide poisoning.

AIM: Carbon monoxide (CO) poisoning can cause permanent damage in tissues that are sensitive to hypoxia. We explored the feasibility and efficacy of using a hyperoxygenated solution (HOS) to treat severe acute CO poisoning in an animal model. METHODS: Male Sprague-Dawley rats were subjected to CO poisoning. The HOS was administered into the femoral vein of these rats through a catheter (10 ml/kg). Carboxyhemoglobin (COHb) and blood gases were used to assess the early damage caused by CO poisoning. S100β was measured to predict the development of late cognitive sequelae of CO. The Morris water maze test was performed to assess cognitive function, and Nissl staining was performed to observe histologic change. RESULTS: The COHb concentrations rapidly decreased at 5 min after the HOS administration; however, the PaO2 and SaO2 in rats treated with HOS increased significantly 5 min after the HOS administration. The S100β concentrations, which increased significantly after CO poisoning, increased at a much slower rate in the rats treated with HOS (HOS group) compared with the rats treated with O2 inhalation (O2 group). The escape latency in the place navigation test was shortened after CO poisoning on days 11-15 and days 26-30, and the swimming time in quadrant 4 in the spatial probe test on days 15 and 30 after CO poisoning was prolonged in the rats treated with HOS injection compared with the rats treated with oxygen inhalation or normal saline injection. The neuronal degeneration in the HOS group was alleviated than that in the CO or O2 group. CONCLUSION: HOS efficiently alleviates the brain damage in acute CO-poisoned rats and thus may serve as a new way to treat human patients with CO poisoning in clinical practice

An Integrated, Real-Time Convective PCR System for Isolation, Amplification, and Detection of Nucleic Acids

Convective PCR (CPCR) can perform rapid nucleic acid amplification by inducing thermal convection to continuously, cyclically driving reagent between different zones of the reactor for spatially separate melting, annealing, and extending in a capillary tube with constant heating temperatures at different locations. CPCR is promoted by incorporating an FTA membrane filter into the capillary tube, which constructs a single convective PCR reactor for both sample preparation and amplification. To simplify fluid control in sample preparation, lysed sample or wash buffer is driven through the membrane filter through centrifugation. A movable resistance heater is used to heat the capillary tube for amplification, and meanwhile, a smartphone camera is adopted to monitor in situ fluorescence signal from the reaction. Different from other existing CPCR systems with the described simple, easy-to-use, integrated, real-time microfluidic CPCR system, rapid nucleic acid analysis can be performed from sample to answer. A couple of critical issues, including wash scheme and reaction temperature, are analyzed for optimized system performance. It is demonstrated that influenza A virus with the reasonable concentration down to 1.0 TCID50/mL can be successfully detected by the integrated microfluidic system within 45 min

Changes in arterial oxygen partial pressure (PaO₂) and arterial oxygen saturation (SaO₂).

<p>The mean PaO₂ levels at 0, 60, 65, 90, 120, and 150 min after acute CO poisoning (A). The mean SaO₂ level at 0, 60, 65, 90, 120, and 150 min after acute CO poisoning (B). The values are means ± SDs (n = 8).<b><i>^*</i></b> comparison of the PaO₂ or SaO₂ values of the CO group with those of the NC group (^** indicates P < 0.01). <b><i>^#</i></b> comparison of the PaO₂ or SaO₂ values of the HOS group with those of the O₂ group (^# and ^## indicate P < 0.05 and P < 0.01, respectively). <b><i>^$</i></b> comparison of the PaO₂ or SaO₂ values of the O₂ group with those of the CO group (^$ and ^$$ indicate P < 0.05 and P < 0.01, respectively).</p