PE-YOLO: Pyramid Enhancement Network for Dark Object Detection

Current object detection models have achieved good results on many benchmark datasets, detecting objects in dark conditions remains a large challenge. To address this issue, we propose a pyramid enhanced network (PENet) and joint it with YOLOv3 to build a dark object detection framework named PE-YOLO. Firstly, PENet decomposes the image into four components of different resolutions using the Laplacian pyramid. Specifically we propose a detail processing module (DPM) to enhance the detail of images, which consists of context branch and edge branch. In addition, we propose a low-frequency enhancement filter (LEF) to capture low-frequency semantics and prevent high-frequency noise. PE-YOLO adopts an end-to-end joint training approach and only uses normal detection loss to simplify the training process. We conduct experiments on the low-light object detection dataset ExDark to demonstrate the effectiveness of ours. The results indicate that compared with other dark detectors and low-light enhancement models, PE-YOLO achieves the advanced results, achieving 78.0% in mAP and 53.6 in FPS, respectively, which can adapt to object detection under different low-light conditions. The code is available at https://github.com/XiangchenYin/PE-YOLO.Comment: Accepted at ICANN 202

DEFormer: DCT-driven Enhancement Transformer for Low-light Image and Dark Vision

The goal of low-light image enhancement is to restore the color and details of the image and is of great significance for high-level visual tasks in autonomous driving. However, it is difficult to restore the lost details in the dark area by relying only on the RGB domain. In this paper we introduce frequency as a new clue into the network and propose a novel DCT-driven enhancement transformer (DEFormer). First, we propose a learnable frequency branch (LFB) for frequency enhancement contains DCT processing and curvature-based frequency enhancement (CFE). CFE calculates the curvature of each channel to represent the detail richness of different frequency bands, then we divides the frequency features, which focuses on frequency bands with richer textures. In addition, we propose a cross domain fusion (CDF) for reducing the differences between the RGB domain and the frequency domain. We also adopt DEFormer as a preprocessing in dark detection, DEFormer effectively improves the performance of the detector, bringing 2.1% and 3.4% improvement in ExDark and DARK FACE datasets on mAP respectively.Comment: submit to ICRA202

Outbreak of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae ST15 in a Chinese teaching hospital: a molecular epidemiological study

Background and AimsThe incidence of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) has been on the rise in China over the past five years, potentially leading to nosocomial epidemics. This study investigates the first outbreak of CRKP in the Second Affiliated Hospital of Jiaxing University.MethodsBetween February 2021 and March 2022, 21 clinical isolates of OXA-232-producing CRKP were recovered from 16 patients in the Second Affiliated Hospital of Jiaxing University. We conducted antimicrobial susceptibility tests, whole genome sequencing, and bioinformatics to determine the drug resistance profile of these clinical isolates.ResultsWhole-genome sequencing revealed that all 21 OXA-232-producing CRKP strains belonged to the sequence type 15 (ST15) and shared similar resistance, virulence genes, and plasmid types, suggesting clonal transmission between the environment and patients. Integrated genomic and epidemiological analysis traced the outbreak to two clonal transmission clusters, cluster 1 and cluster 2, including 14 and 2 patients. It was speculated that the CRKP transmission mainly occurred in the ICU, followed by brain surgery, neurosurgery, and rehabilitation department. Phylogenetic analysis indicated that the earliest outbreak might have started at least a year before the admission of the index patient, and these strains were closely related to those previously isolated from two major adjacent cities, Shanghai and Hangzhou. Comparative genomics showed that the IncFII-type and IncHI1B-type plasmids of cluster 2 had homologous recombination at the insertion sequence sites compared with the same type of plasmids in cluster 1, resulting in the insertion of 4 new drug resistance genes, including TEM-1, APH(6)-Id, APH(3’’)-Ib and sul2.ConclusionsOur study observed the clonal spread of ST15 OXA-232-producing between patients and the hospital environment. The integration of genomic and epidemiological data offers valuable insights and facilitate the control of nosocomial transmission

The Motion of An Inv Nodal Cilium: a Realistic Model Revealing Dynein-Driven Ciliary Motion with Microtubule Mislocalization

Background/Aims: Nodal cilia that rotate in the ventral node play an important role in establishing left-right asymmetry during embryogenesis; however, inv mutant cilia present abnormal movement and induce laterality defects. The mechanism of their motility, which is regulated by dynein activation and microtubule arrangement, has not been fully understood. This study analyzed the dynein-triggered ciliary motion in the abnormal ultrastructure of the inv mutant, aiming to quantitatively evaluate the influence of microtubule mislocalization on the movement of the cilium. Methods: We established a realistic 3-D model of an inv mutant cilium with an ultrastructure based on tomographic datasets generated by ultra-high voltage electron microscopy. The time-variant activation of the axonemal dynein force was simulated by pairs of point loads and embedded at dynein-mounted positions between adjacent microtubule doublets in this mathematical model. Utilizing the finite element method and deformable grid, the motility of the mutant cilium that is induced by various dynein activation hypotheses was investigated and compared to experimental observation. Results: The results indicate that for the inv mutant, simulations of the ciliary movement with the engagement of dyneins based on the distance-controlled pattern in the partially activation scenario are broadly consistent with the observation; the shortening of the microtubules induces smaller movement amplitudes, while the angles of the mislocalized microtubules affect the pattern of the ciliary movement, and during the ciliary movement, the microtubules swing and twist in the mutant ciliary body. Conclusion: More generally, this study implies that dynein engagement is sensitive to subtle geometric changes in the axoneme, and thus, this geometry greatly influences the integrity of a well-formed ciliary rotation

Investigation of two-phase flow mixing mechanism of a swirl burner using an electrostatic sensor array system

A novel electrostatic sensor array was designed to measure particle concentration downstream of a swirl burner. The fundamental mechanism and the primary constituent elements of the sensor array were described. The root-mean-square magnitude of the measured electrostatic voltage was determined as an indication of the particle concentration. The accuracy of the electrostatic sensor array was calibrated by the optical fluctuation method. Local particle concentrations at different cross-sections of the measuring chamber were measured to investigate the diffusion characteristic of the pulverized coal particles. Electrostatic sensor array showed its ability in the field measurement in this work. The measurements indicated that the velocity of the inner secondary air had a significant effect on the diffusion of the pulverized coal particles. The particles concentrated in the center of the cross-section after leaving the burner. With the development of the gas–solid two-phase flow, the particles distributed like a ring shape. The radius of the particle ring increased with the increase of the velocity of the inner secondary air. But the effect of the velocity of outer secondary air on the radius of the particle ring is very slight. The maximum radius occurred when the velocity of inner secondary air was 21 m/s, which was favorable for stable combustion

Binge-Like Sucrose Self-Administration Experience Inhibits Cocaine and Sucrose Seeking Behavior in Offspring

Recent studies show that emotional and environmental stimuli promote epigenetic inheritance and influence behavioral development in the subsequent generations. Caloric mal- and under-nutrition has been shown to cause metabolic disturbances in the subsequent generation, but the incentive properties of paternal binge-like eating in offspring is still unknown. Here we show that paternal sucrose self-administration experience could induce inter-generational decrease in both sucrose and cocaine-seeking behavior, and sucrose responding in F1 rats, but not F2, correlated with the performance of F0 rats in sucrose self-administration. Higher anxiety level and decreased cocaine sensitivity were observed in Sucrose F1 compared with Control F1, possibly contributing to the desensitization phenotype in cocaine and sucrose self-administration. Our study revealed that paternal binge-like sucrose consumption causes decrease in reward seeking and induces anxiety-like behavior in the F1 offspring

Multiple functions of reversine on the biological characteristics of sheep fibroblasts

Abstract Previous reports have demonstrated that Reversine can reverse differentiation of lineage-committed cells to mesenchymal stem cells and suppress tumors growth. However, the molecular mechanisms of antitumor activity and promoting cellular dedifferentiation for reversine have not yet been clearly elucidated. In the present study, it was demonstrated that reversine of 5 μM could induce multinucleated cells through cytokinesis failure rather than just arrested in G2 or M phase. Moreover, reversine reversed the differentiation of sheep fibroblasts into MSC-like style, and notably increased the expression of pluripotent marker genes Oct4 and MSCs-related surface antigens. The fibroblasts treated with reversine could transdifferentiate into all three germ layers cells in vitro. Most importantly, the induced β-like cells and hepatocytes had similar metabolic functions with normal cells in vivo. In addition, reversine promoted fibroblasts autophagy, ROS accumulation, mitochondrial dysfunction and cell apoptosis via the mitochondria mediated intrinsic pathway. The results of high-throughput RNA sequencing showed that most differentially expressed genes (DEGs) involved in Mismatch repair, Nucleotide excision repair and Base excision repair were significantly up-regulated in reversine treated fibroblasts, which means that high concentration of reversine will cause DNA damage and activate the DNA repair mechanism. In summary, reversine can increase the plasticity of sheep fibroblasts and suppress cell growth via the mitochondria mediated intrinsic pathway

Construction of a Full-Length Enriched cDNA Library and Preliminary Analysis of Expressed Sequence Tags from Bengal Tiger Panthera tigris tigris

In this study, a full-length enriched cDNA library was successfully constructed from Bengal tiger, Panthera tigris tigris, the most well-known wild Animal. Total RNA was extracted from cultured Bengal tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.28 × 106 pfu/mL and 1.56 × 109 pfu/mL respectively. The percentage of recombinants from unamplified library was 90.2% and average length of exogenous inserts was 0.98 kb. A total of 212 individual ESTs with sizes ranging from 356 to 1108 bps were then analyzed. The BLASTX score revealed that 48.1% of the sequences were classified as a strong match, 45.3% as nominal and 6.6% as a weak match. Among the ESTs with known putative function, 26.4% ESTs were found to be related to all kinds of metabolisms, 19.3% ESTs to information storage and processing, 11.3% ESTs to posttranslational modification, protein turnover, chaperones, 11.3% ESTs to transport, 9.9% ESTs to signal transducer/cell communication, 9.0% ESTs to structure protein, 3.8% ESTs to cell cycle, and only 6.6% ESTs classified as novel genes. By EST sequencing, a full-length gene coding ferritin was identified and characterized. The recombinant plasmid pET32a-TAT-Ferritin was constructed, coded for the TAT-Ferritin fusion protein with two 6× His-tags in N and C-terminal. After BCA assay, the concentration of soluble Trx-TAT-Ferritin recombinant protein was 2.32 ± 0.12 mg/mL. These results demonstrated that the reliability and representativeness of the cDNA library attained to the requirements of a standard cDNA library. This library provided a useful platform for the functional genome and transcriptome research of Bengal tigers

Effect of Different Sowing Dates in South Henan's Rice-growing Areas on the Growth and Yield of Ratoon Rice

In order to determine the optimal sowing date of ratoon rice in South Henan's rice-growing areas, this paper performs a comparative analysis of rice growth process, seedling quality and yield of first season rice and ratoon rice under different sowing date treatments. The results show that under climatic conditions (2014), by using dry seedling cultivation in a small plastic shed, the growth of seedling sown on February 21 was affected, while the sowing treatments from March 1 to April 11 can breed normal seedlings, and in this period, the maturity period of first season rice was delayed with prolonged sowing date, and ratoon rice yield declined with prolonged sowing date (total production of rice sown on 11 March reaching a peak). Thus, it is considered that the optimal sowing date of ratoon rice in South Henan's rice-growing areas is mid-March