Efficient and Scalable Listing of Four-Vertex Subgraph

Identifying four-vertex subgraphs has long been recognized as a fundamental technique in bioinformatics and social networks. However, listing these structures is a challenging task, especially for graphs that do not fit in RAM. To address this problem, we build a set of algorithms, models, and implementations that can handle massive graphs on commodity hardware. Our technique achieves 4 – 5 orders of magnitude speedup compared to the best prior methods on graphs with billions of edges, with external-memory operation equally efficient

Efficient and Scalable Listing of Four-Vertex Subgraph

Identifying four-vertex subgraphs has long been recognized as a fundamental technique in bioinformatics and social networks. However, listing these structures is a challenging task, especially for graphs that do not fit in RAM. To address this problem, we build a set of algorithms, models, and implementations that can handle massive graphs on commodity hardware. Our technique achieves 4 – 5 orders of magnitude speedup compared to the best prior methods on graphs with billions of edges, with external-memory operation equally efficient

Comparative Study on Curcumin Loaded in Golden Pompano (Trachinotus blochii) Head Phospholipid and Soybean Lecithin Liposomes: Preparation, Characteristics and Anti-Inflammatory Properties

In this study, we compared the characteristics and in vitro anti-inflammatory effects of two curcumin liposomes, prepared with golden pompano head phospholipids (GPL) and soybean lecithin (SPC). GPL liposomes (GPL-lipo) and SPC liposomes (SPC-lipo) loaded with curcumin (CUR) were prepared by thin film extrusion, and the differences in particle size, ζ-potential, morphology, and storage stability were investigated. The results show that GPL-lipo and SPC-lipo were monolayer liposomes with a relatively small particle size and excellent encapsulation rates. However, GPL-lipo displayed a larger negative ζ-potential and better storage stability compared to SPC-lipo. Subsequently, the effects of phospholipids in regulating the inflammatory response of macrophages were evaluated in vitro, based on the synergistic effect with CUR. The results showed that both GPL and SPC exerted excellent synergistic effect with CUR in inhibiting the lipopolysaccharide (LPS)-induced secretion of nitric oxide (NO), reactive oxygen species (ROS), and pro-inflammatory genes (tumor necrosis factor (TNF)-α, interleukin 1β (IL-β), and interleukin 6 (IL-6)) in RAW264.7 cells. Interestingly, GPL-lipo displayed superior inhibitory effects, compared to SPC-lipo. The findings provide a new innovative bioactive carrier for development of stable CUR liposomes with good functional properties

Performance of the Cold-Bending Channel-Angle Buckling-Restrained Brace under Cyclic Loading

In this study, three restricted cold-bending channel-angle buckling-restrained brace (CCA-BRB) specimens were experimentally characterised by a low-reversed cyclic loading test. Three specimens had steel cores with cruciform cross section. Two restraining units were assembled to form an external constraint member, each of which was composed of an equilateral cold-bending channel and two equilateral cold-bending angles via welding. A gap or a thin silica gel plate was set between the internal core and the external constraint member to form an unbonded layer. Several evaluation parameters on the seismic performance, hysteretic behaviour, and energy dissipation capability of the CCA-BRB was investigated, including hysteresis curve, skeleton curve, compression strength adjustment factor, measured and computed stiffness, energy dissipation coefficient, equivalent viscous damping ratio, ductility coefficient, and cumulative plastic deformation. The test results and evaluation indices demonstrated that the hysteretic performance of braces with a rigid connection was stable. A Ramberg–Osgood model and two model parameters were calibrated to predict, with fidelity, the skeleton curve of CCA-BRB under cyclic load. The initial elastic stiffness of the brace used in practice should contain overall portions of the brace instead of the yielding portion of the brace. Finally, all the tested CCA-BRBs exhibited a stable energy absorption performance and verified the specimens’ construction was rational

Tilapia-Head Chondroitin Sulfate Protects against Nonalcoholic Fatty Liver Disease via Modulating the Gut–Liver Axis in High-Fat-Diet-Fed C57BL/6 Mice

We isolated and characterized tilapia-head chondroitin sulfate (TH-CS) and explored its biological activity and mechanisms of action as an oral supplement for nonalcoholic fatty liver disease (NAFLD) induced by a high-fat diet (HFD) in mice. The results showed that treatment with TH-CS for 8 weeks alleviated the development of NAFLD, as evidenced by the notable improvement in liver damage, blood lipid accumulation and insulin resistance (IR). Meanwhile, TH-CS treatment reduced the expression of proinflammatory cytokines and normalized oxidative stress. Additionally, the analysis of 16S rDNA sequencing revealed that TH-CS could restore gut microbiota balance and increase the relative abundance of short-chain fatty acid (SCFA)-producing bacteria. Furthermore, SCFAs produced by related bacteria can further improve lipid metabolism and IR by regulating lipid synthesis signals. In conclusion, TH-CS is an effective dietary supplement for the prevention of NAFLD, and may serve as a potential supplementary treatment for lipid-related metabolic syndrome

Multi-targeting inulin-based nanoparticles with cannabidiol for effective prevention of ulcerative colitis

The pathogenesis of ulcerative colitis (UC) is closely related to severe inflammation, damaged colonic mucosal barrier, increased oxidative stress and intestinal ecological imbalance. However, due to the nonspecific distribution and poor bioavailability of drugs, UC treatment is still a serious challenge. Here, a mitochondria/colon dual targeted nanoparticles based on redox response was developed to effectively alleviate UC. Cannabidiol nanoparticles (CBD NPs) with a particle size of 143.2 ± 3.11 nm were prepared by self-assembly using polymers (TPP–IN–LA) obtained by modifying inulin with (5-carboxypentyl) triphenyl phosphonium bromide (TPP) and α-lipoic acid (α-LA). Excitingly, the constructed CBD NPs showed excellent mitochondrial targeting, with a Pearson correlation coefficient of 0.76 at 12 h. The results of animal imaging in vivo showed that CBD NPs could be effectively accumulated in colon tissue. Not only that, CBD showed significant glutathione stimulated release in the presence of 10 mM glutathione at pH 7.4. The results of in vivo animal experiments showed that CBD NPs significantly ameliorated DSS-induced colonic inflammation by modulating the TLR4-NF-κB signaling pathway. Moreover, CBD NPs significantly improved the histological damage of colon in UC mice, increased the expression level of tight junction protein ZO-1, and effectively restored the intestinal mucosal barrier function and intestinal mucosal permeability. More importantly, CBD NPs significantly improved the species composition, abundance and amount of short chain fatty acids of intestinal flora in UC mice, thus effectively maintaining the balance of intestinal flora. The dual-targeted and glutathione-responsive nanoparticles prepared in this study provide a promising idea for achieving targeted delivery of CBD for effective treatment of UC

New insight into the effects of Ca(II) on cake layer structure in submerged membrane bioreactors

<div><p>The effects of Ca(II) on the structure of the cake layer in submerged membrane bioreactors (SMBRs) were investigated in this study. Three parallel laboratory-scale SMBRs were operated with synthetic municipal wastewater with three Ca(II) levels (82, 208 and 410 mg l⁻¹). As the Ca(II) concentration increased, the sludge floc size increased and the molecular weight of the soluble microbial products (SMP) in the bulk liquid decreased. These observations were attributed to the neutralization and bridging function of Ca(II). Furthermore, Ca(II) addition did not change the thickness of the cake layer, but inhibited the deposition of other elements, such as Al, Si, Mg, and Fe. As a result of Ca(II) addition, the cake layer became less compact and more porous. The interspaces among the flocs in the cake layer helped to reduce the membrane fouling potential.</p></div

Effects of <i>Mactra chinenesis</i> Peptides on Alcohol-Induced Acute Liver Injury and Intestinal Flora in Mice

Food-borne bioactive peptides have shown promise in preventing and mitigating alcohol-induced liver injury. This study was the first to assess the novel properties of Mactra chinenesis peptides (MCPs) in mitigating acute alcoholic liver injury in mice, and further elucidated the underlying mechanisms associated with this effect. The results showed that MCPs can improve lipid metabolism by modulating the AMPK signaling pathway, decreasing fatty acid synthase activity, and increasing carnitine palmitoyltransferase 1a activity. Meanwhile, MCPs ameliorate inflammation by inhibiting the NF-κB activation, leading to reduced levels of pro-inflammatory cytokines (tumor necrosis factor-α and interleukin-1β). Additionally, a 16S rDNA sequencing analysis revealed that MCPs can restore the balance of gut microbiota and increase the relative abundance of beneficial bacteria. These findings suggest that supplementation of MCPs could attenuate alcohol intake-induced acute liver injury, and, thus, may be utilized as a functional dietary supplement for the successful treatment and prevention of acute liver injury

Disorder analytic model-based CMT algorithms in vehicular sensor networks

Recently, vehicular sensor networks (VSNs) have emerged as a new intelligent transport networking paradigm in the Internet of Things. By sensing, collecting, and delivering traffic-related information, VSNs can significantly improve both driving experience and traffic flow control, especially in constrained urban environments. Latest technological advances enable vehicular devices to be equipped with multiple wireless interfaces, which can support cooperative communications for concurrent multipath transfer (CMT) in VSNs. However, path heterogeneity and vehicle mobilitycause CMT not to achieve the same high transport efficiency recorded in wired nonmobile network environments. This paper proposes a novel vehicular network-based CMT solution (VNCMT) to address the above issues and improve data delivery efficiency. VN-CMT is based on a CMTdisorder analytic model which can effectively and accurately evaluate the degree of out-of-order data. Based on this proposed model, a series of mechanisms are introduced as follows: (1) a packet disorder-reducing retransmission policy to reduce retransmission delay; (2) a path group selection algorithm to find the best path set for data multipath concurrent transfer; and (3) a data scheduling mechanism to distribute data according to each path’s capacity. Simulation results show how VN-CMT improves data delivery efficiency in comparison with an existing state-of-the-art solution