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Characterization of Cell Glycocalyx with Mass Spectrometry Methods.
The cell membrane plays an important role in protecting the cell from its extracellular environment. As such, extensive work has been devoted to studying its structure and function. Crucial intercellular processes, such as signal transduction and immune protection, are mediated by cell surface glycosylation, which is comprised of large biomolecules, including glycoproteins and glycosphingolipids. Because perturbations in glycosylation could result in dysfunction of cells and are related to diseases, the analysis of surface glycosylation is critical for understanding pathogenic mechanisms and can further lead to biomarker discovery. Different mass spectrometry-based techniques have been developed for glycan analysis, ranging from highly specific, targeted approaches to more comprehensive profiling studies. In this review, we summarized the work conducted for extensive analysis of cell membrane glycosylation, particularly those employing liquid chromatography with mass spectrometry (LC-MS) in combination with various sample preparation techniques
The long-term optical behavior of MRK421
All data available in B band for the BL Lac object MRK421 from 22
publications are used to construct a historical light curve, dating back to
1900. It is found that the light curve is very complicated and consists of a
set of outbursts with very large duration. The brightness of MRK421 varies from
11.6 magnitude to more than 16 magnitude. Analyses with Jurkevich method of
computing period of cyclic phenomena reveal in the light curve two kinds of
behaviors. The first one is non-periodic with rapid, violent variations in
intensity on time scales of hours to days. The second one is periodic with a
possible period of years. Another possible period of years is not very significant. We have tested the robustness of the
Jurkevich method. The period of about one year found in the light curves of
MRK421 and of other objects is a spurious period due to the method and the
observing window. We try to explain the period of years under the
thermal instability of a slim accretion disk around a massive black hole of
mass of .Comment: Tex, 14 pages, 5 Postscript figures. Accepted for publication in A&A
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Identification of potential sialic acid binding proteins on cell membranes by proximity chemical labeling.
The cell membrane contains a highly interactive glycan surface on a scaffold of proteins and lipids. Sialic acids are negatively charged monosaccharides, and the proteins that bind to sialic acids play an important role in maintaining the integrity and collective functions of this interactive space. Sialic acid binding proteins are not readily identified and have nearly all been discovered empirically. In this research, we developed a proximity labeling method to characterize proteins with oxidation by localized radicals produced in situ. The sites of oxidation were identified and quantified using a standard proteomic workflow. In this method, a clickable probe was synthesized and attached to modified sialic acids on the cell membrane, which functioned as a catalyst for the localized formation of radicals from hydrogen peroxide. The proteins in the sialic acid environment were labeled through amino acid oxidation, and were categorized into three groups including sialylated proteins, non-sialylated proteins with transmembrane domains, and proteins that are associated with the membrane with neither sialylated nor transmembrane domains. The analysis of the last group of proteins showed that they were associated with binding functions including carbohydrate binding, anion binding, and cation binding, thereby revealing the nature of the sialic acid-protein interaction. This new tool identified potential sialic acid-binding proteins in the extracellular space and proteins that were organized around sialylated glycans in cells
Tunable Dipolar Capillary Deformations for Magnetic Janus Particles at Fluid-Fluid Interfaces
Janus particles have attracted significant interest as building blocks for
complex materials in recent years. Furthermore, capillary interactions have
been identified as a promising tool for directed self-assembly of particles at
fluid-fluid interfaces. In this paper, we develop theoretical models describing
the behaviour of magnetic Janus particles adsorbed at fluid-fluid interfaces
interacting with an external magnetic field. Using numerical simulations, we
test the models predictions and show that the magnetic Janus particles deform
the interface in a dipolar manner. We suggest how to utilise the resulting
dipolar capillary interactions to assemble particles at a fluid-fluid
interface, and further demonstrate that the strength of these interactions can
be tuned by altering the external field strength, opening up the possibility to
create novel, reconfigurable materials.Comment: 9 pages, 7 figure
Nonperturbative signatures in pair production for general elliptic polarization fields
The momentum signatures in nonperturbative multiphoton pair production for
general elliptic polarization electric fields are investigated by employing the
real-time Dirac-Heisenberg-Wigner formalism. For a linearly polarized electric
field we find that the positions of the nodes in momenta spectra of created
pairs depend only on the electric field frequency. The polarization of external
fields could not only change the node structures or even make the nodes
disappear but also change the thresholds of pair production. The momentum
signatures associated to the node positions in which the even-number-photon
pair creation process is forbid could be used to distinguish the orbital
angular momentum of created pairs on the momenta spectra. These distinguishable
momentum signatures could be relevant for providing the output information of
created particles and also the input information of ultrashort laser pulses.Comment: 8 pages, 4 figures, submitted to Europhysics Letter
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