Transformujący czynnik wzrostu beta w raku podstawnokomórkowym, kolczystokomórkowym i rogowiaku kolczystokomórkowym

Introduction. Transforming Growth Factor β (TGFβ) activates signaling cascades which regulate cell proliferation, differentiation, apoptosis, inflammatory response and angiogenesis. In the early stages of malignant transformation this cytokine acts as an inhibitor of tumour growth. In the advanced stages of malignant transformation TGFβ acts as a promoter of metastasis. Changes in the expression of genes associated with TGFβactivity could provide a new strategy of molecularly targeted therapy.Aim. The aim of this study was to compare the mRNA profile of genes associated with TGFβ signaling pathways in non-melanoma skin pathologies biopsy specimens of bas-al cell carcinoma (BCC), squamous cell carcinoma (SCC) and keratoacanthoma (KA) in comparison to normal skin.Material and methods. Tissue samples of KA, SCC and BCC were obtained from the central part of tumours. Healthy skin margins comprised the control group. mRNA profile of genes coding TGFβ and proteins involved in TGFβ-induced signaling pathways was determined using oligonucleotide microarrays (Affymetrix).Results. Microarray analysis showed changes in profile of genes coding proteins in-volved in TGFβ-induced signaling pathways. In SCC TGFβ-1 (TGFB1) was upregulated, comparing to controls. Both in KA and SCC, the most statistically significant change re-ferred to TGFBR3 (Transforming Growth Factor beta Receptor III) mRNA.Conclusions. mRNA profile of genes coding proteins involved in TGFβ-induced signal-ization reveals strong molecular similarity of SCC and KA

Poziom ekspresji defensyny DEFB4A w różnicowaniu rogowiaka kolczystokomórkowego, raka kolczystokomórkowego i raka podstawnokomórkowego

Introduction. Defensins are peptide with antimicrobial, antiviral, antifungal activities and many other functions, such as induction of immunological response and antitumor activity. Changes in expression level of defensins was studied in many skin pathologies, including dermatological lesions such as psoriasis, atopic dermatitis and non-melanoma skin cancers (squamous cell carcinoma – SCC and basal cell carcinoma – BCC).Aim. The objective of this study was to evaluate the mRNA profile of defensin-related genes’ transcripts as an additional molecular marker of non-melonoma skin pathologies: SCC, BCC and keratoacanthoma (KA).Material and methods. Tissue samples were obtained from the central part of tumours (KA, SCC and BCC) and healthy margins. mRNA profile of genes coding defensins and proteins involved in their activation was determined using oligonucleotide microarrays (Affymetrix). Vali-dation of the microarray analysis was performed using real-time QRT-PCR.Results. Microarray analysis revealed changes in defensin-related genes’ profile. In all tumours DEFB4A (defensin beta 2) mRNA was up-regulated, compared with the healthy skin margins. Real-time QRT-PCR analysis showed increased DEFB4A transcript level both in KA and SCC comparing to BCC.Conclusions. Defensin beta 2 mRNA level is a useful tool for the differentiation of KA and SCC from BCC. KA and SCC cannot be differentiated on the basis of the DEFB4AmRNA level

Przerzuty czerniaka złośliwego do skóry brzucha i klatki piersiowej imitujące infekcję wirusem herpes zoster – opis przypadku

Introduction. Melanoma is a malignant neoplasm of melanocytic origin.The risk of melanoma among the white race in Europe and USA isfrom 1/100 000 to 1/200 000.Objective. To present a case with multiple melanoma metastases to theskin of the thorax and abdomen with an atypical clinical picture mimickingherpes zoster infection.Case report. Male patient 77 years old. The primary lesion – a darkbrown nodule, 2 cm in diameter – appeared half a year earlier. Underlocal anaesthesia, the nodule was removed with a 3 cm skin margin.Two months later, multiple, non-painful, brown nodules appeared onthe left part of the chest and abdomen and were diagnosed as herpeszoster. The histopathological examination revealed melanoma metastases.Conclusions. We present a case of very fast appearance of malignantmelanoma metastases, resembling herpes zoster infection. Althoughsuch a picture is rare, it is important to take this diagnosis into accountin patients with “zoster” with no pain, resistant to antiviral treatment,and especially in patients with known melanoma history