Эвакуация населения при чрезвычайных ситуациях

В результате аварии на Чернобыльской АЭС (1986 г.) радиационное загрязнение затронуло все страны Северного полушария. На опасно загрязненных территориях оказалось более 1 млн. чел. В ряду поколений число смертельных онкологических заболеваний может составить десятки тысяч человек.As a result of the Chernobyl accident (1986), all countries of the Northern Hemisphere were affected by radiation pollution. In dangerously contaminated areas was more than 1 million people. In a number of generations, the number of deadly cancer can amount to tens of thousands of people

Specific features of petrophysical properties of complex carbonate reservoir by complex geophysical data

Актуальность исследования определена необходимостью прогнозирования латеральной изменчивости литологических типов пород, что оказывает влияние на разработку и экономическую оценку месторождения. Очень часто нефтяные компании имеют ограниченный набор геолого-геофизической информации, в связи с чем возникают трудности для прогнозирования перспективных областей, что способствует увеличению неопределенностей при изучении территории месторождения. Цель: выделение и статистическое обоснование фациального строения по данным геолого-геофизической информации на примере одного из месторождений Томской области, приуроченного к палеозойскому фундаменту Западной Сибири. Объектом исследования являются отложения палеозойского фундамента юго-восточной части Западно-Сибирской плиты - коллекторы сложнопостроенных карбонатных толщ, окно исследования, которое охватывает объем горной породы, равный 40 м, выбрано исходя из условий построения сейсмофациальной карты. Методы исследования основаны на использовании комплексного анализа данных - статистическое сопоставление геофизических исследований и кернового материала. Такой анализ способствует уменьшению неопределенностей в процессе оценки и прогноза геологической среды. В работе отмечено, что использование широкого спектра информации влияет на оценку и прогноз распределения геологических тел в пространстве. Предложена методика сравнения акустических свойств, полученных из первоначального источника - сейсмические данные, с расчетными акустическими свойствами по керновым данным. Выявлены взаимосвязи между сейсмической, геофизической информацией и лабораторными исследованиями керна. Обоснование полученной сейсмофациальной карты на основе комплексирования данных позволяет эффективно прогнозировать геологическое распределение фаций в пространстве и времени, но также уменьшать неопределенности при построении геологической модели.The relevance of the research is determined by the need to predict the lateral variability of lithological rock types, which affects the development and economic evaluation of the field. Very often, oil companies have a limited set of geological and geophysical information, in this relation the difficulties arise in predicting promising areas, which contributes to increase in uncertainties in the study of the field. The main aim of the research is isolation and statistical justification of the facies structure according to the geological and geophysical information on the example of one of the deposits of the Tomsk region, confined to the Paleozoic basement of Western Siberia. The object of the study is deposits of the Paleozoic basement of the southeastern part of the West Siberian Plate - complex carbonate reservoir, the study window that covers a rock volume of 40 m is selected based on the conditions for constructing a seismic facies map. Methods of the research are based on the use of integrated data analysis - a statistical comparison of geophysical surveys and core material. This analysis helps to reduce uncertainties in the process of assessing and forecasting the geological environment. The paper notes that the use of a wide range of information affects the assessment and forecast of the distribution of geological bodies in space. A technique is proposed for comparing acoustic properties obtained from the original source - seismic data, with the calculated acoustic properties from core data. The authors have revealed the interconnections between seismic, geophysical information and core research. The justification of the obtained seismic facies map based on the data integration allows us to effectively predict the geological distribution of facies in space and time, but also to reduce uncertainties in the construction of the geological model

Flow cytometry fluorescence levels showing the expression of (A) CD44 and (B) CD147.

<p>Cell pellet immunohistochemistry showing the expression of (C) CD44 and (D) CD147. HOS and POS are the non- metastatic human and canine cell lines and 143B and HMPOS are the metastatic human and canine cell lines respectively. Details of quantification are indicated in SI.</p

Comparative proteomic investigation of metastatic and non-metastatic osteosarcoma cells of human and canine origin

<div><p>Osteosarcoma is the most common bone cancer in dogs and people. In order to improve clinical outcomes, it is necessary to identify proteins that are differentially expressed by metastatic cells. Membrane bound proteins are responsible for multiple pro-metastatic functions. Therefore characterizing the differential expression of membranous proteins between metastatic and non-metastatic clonal variants will allow the discovery of druggable targets and consequently improve treatment methodology. The objective of this investigation was to systemically identify the membrane-associated proteomics of metastatic and non-metastatic variants of human and canine origin. Two clonal variants of divergent <i>in vivo</i> metastatic potential from human and canine origins were used. The plasma membranes were isolated and peptide fingerprinting was used to identify differentially expressed proteins. Selected proteins were further validated using western blotting, flow cytometry, confocal microscopy and immunohistochemistry. Over 500 proteins were identified for each cell line with nearly 40% of the proteins differentially regulated. Conserved between both species, metastatic variants demonstrated significant differences in expression of membrane proteins that are responsible for pro-metastatic functions. Additionally, CD147, CD44 and vimentin were validated using various biochemical techniques. Taken together, through a comparative proteomic approach we have identified several differentially expressed cell membrane proteins that will help in the development of future therapeutics.</p></div

Fig shows global membrane proteomic composition across various cell lines classified according to function.

<p>The colors are as mentioned in the key. HOS and POS are the non- metastatic human and canine cell lines and 143B and HMPOS are the metastatic human and canine cell lines respectively.</p

Table shows the relative emPAI of selected differentially regulated proteins in the human and canine osteosarcoma cells.

<p>HOS and POS are the non-metastatic human and canine cell lines and 143B and HMPOS are the metastatic human and canine cell lines respectively. emPAI of each protein is normalized to total emPAI. The ratios 143B/HOS and HMPOS/POS show fold change in expression.</p

Table shows the percentage of proteins in each category in each cell membrane.

<p>Table 1 represents the calculations reflected in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183930#pone.0183930.g001" target="_blank">Fig 1</a>. HOS and POS are the non-metastatic human and canine cell lines and 143B and HMPOS are the metastatic human and canine cell lines respectively.</p

Fig shows the relative GPCR activity as measured by cAMP concentration.

<p>The experiment was performed in biological triplicates (and technical duplicates) with n = 2 (total separate trials). **** p <0.0001 and ** p<0.01.</p