Study on Failure Length of Cementing Interface in Horizontal Wells During Fracturing

The cementing interface of oil and gas wells is often the weak link between oil and gas turbulence. Due to the low cementation strength at the fracturing interface, the two interfaces have been crushed to form turbulence channels before the target layer is opened during fracturing. If the closure is not good, there will be inter-layer channeling. Therefore, the pressure bearing capacity of the fracturing interface is an important indicator for designing the fracturing construction parameters. The pressure capacity of the two interfaces during the fracturing process is the key to evaluating the success of the fracturing construction. This paper establishes the calculation model for the stress distribution of horizontal wells in horizontal wells under the effect of non-uniform stress. At the same time, the influence of the pressure change in the wellbore during the fracturing process on the stress distribution in the borehole wall was analyzed. The calculation model of the interfacial stress distribution in the horizontal well during the fracturing process was established, and the debonding pressure and debonding length of the two interfaces under different cementing strengths were calculated. After the establishment of the horizontal well fracturing two interface crack propagation mechanics model, calculate the pressure required for cracks along the two interfaces to expand at different failure lengths

Comparative study of differentiating human pluripotent stem cells into vascular smooth muscle cells in hydrogel-based culture methods

Vascular smooth muscle cells (VSMCs), which provides structural integrity and regulates the diameter of vasculature, are of great potential for modeling vascular-associated diseases and tissue engineering. Here, we presented a detailed comparison of differentiating human pluripotent stem cells (hPSCs) into VSMCs (hPSCs-VSMCs) in four different culture methods, including 2-dimensional (2D) culture, 3-dimensional (3D) PNIPAAm-PEG hydrogel culture, 3-dimensional (3D) alginate hydrogel culture, and transferring 3- dimensional alginate hydrogel culture to 2-dimensional (2D) culture. Both hydrogel-based culture methods could mimic in vivo microenvironment to protect cells from shear force, and avoid cells agglomeration, resulting in the extremely high culture efficiency (e.g., high viability, high purity and high yield) compared with 2D culture. We demonstrated hPSC-VSMCs produced from hydrogel-based culture methods had better contractile phenotypes and the potential of vasculature formation. The transcriptome analysis showed the hPSC-VSMCs derived from hydrogel-based culture methods displayed more upregulated genes in vasculature development, angiogenesis and blood vessel development, extracellular matrix compared with 2D culture. Taken together, hPSC-VSMCs produced from hydrogel-based culture system could be applied in various biomedical fields, and further indicated the suitable development of alginate hydrogel for industrial production by taking all aspects into consideration

The associations between body dissatisfaction, exercise intensity, sleep quality, and depression in university students in southern China

BackgroundIn recent years, depression in early adulthood has become an urgent global public health concern. The university years mark a transitional period from adolescence to adulthood. Young people are required to face academic and life pressures independently, which increases the risk of mental health problems in university.PurposeThe main goal of the current study was to explore the sex differences in depression, body dissatisfaction, sleep quality, and exercise intensity among university students in southern China and to analyze the factors affecting the level of depression among university students.MethodsIn total, 1,258 university students aged 18–23 years were recruited for this study. All participants completed anthropometric measurements, the Self-rating Depression Scale, Physical Activity Rating Scale, and Pittsburgh Sleep Quality Index. Body dissatisfaction levels were measured using sex-appropriate silhouettes.ResultsCompared with young women, young men had higher exercise intensity and sleep quality, whereas young women’s body dissatisfaction and depression levels were significantly higher than those of young men. Sleep quality score (β = 0.34, p < 0.01), sex (β = 0.15, p < 0.01), physical activity score (β = −0.14, p < 0.01), and body dissatisfaction (β = 0.14, p < 0.01) were significant predictive factors of the Self-rating Depression Scale score.ConclusionLow levels of physical dissatisfaction have a positive effect on depression, and high levels of physical activity and quality sleep can also improve depressive symptoms. At the same time, increasing body satisfaction has the effect of increasing physical activity and improving sleep quality. Therefore, there is great potential to prevent and ameliorate depression by reducing body dissatisfaction

Kinetic analysis of paramyxovirus-sialoglycan receptor interactions reveals virion motility

Many viruses initiate infection by binding to sialoglycan receptors at the cell surface. Binding to such receptors comes at a cost, however, as the sheer abundance of sialoglycans e.g. in mucus, may immobilize virions to non-functional decoy receptors. As a solution, sialoglycan-binding as well as sialoglycan-cleavage activities are often present in these viruses, which for paramyxoviruses are combined in the hemagglutinin-neuraminidase (HN) protein. The dynamic interactions of sialoglycan-binding paramyxoviruses with their receptors are thought to be key determinants of species tropism, replication and pathogenesis. Here we used biolayer interferometry to perform kinetic analyses of receptor interactions of animal and human paramyxoviruses (Newcastle disease virus, Sendai virus, and human parainfluenza virus 3). We show that these viruses display strikingly different receptor interaction dynamics, which correlated with their receptor-binding and -cleavage activities and the presence of a second sialic acid binding site. Virion binding was followed by sialidase-driven release, during which virions cleaved sialoglycans until a virus-specific density was reached, which was largely independent of virion concentration. Sialidase-driven virion release was furthermore shown to be a cooperative process and to be affected by pH. We propose that paramyxoviruses display sialidase-driven virion motility on a receptor-coated surface, until a threshold receptor density is reached at which virions start to dissociate. Similar motility has previously been observed for influenza viruses and is likely to also apply to sialoglycan-interacting embecoviruses. Analysis of the balance between receptor-binding and -cleavage increases our understanding of host species tropism determinants and zoonotic potential of viruses

Comparative Study of Human Pluripotent Stem Cell-Derived Endothelial Cells in Hydrogel-Based Culture Systems

Human pluripotent stem cell (hPSC)-derived endothelial cells (ECs) are promising cell sources for drug discovery, tissue engineering, and studying or treating vascular diseases. However, hPSC-ECs derived from different culture methods display different phenotypes. Herein, we made a detailed comparative study of hPSC-ECs from three different culture systems (e.g., 2D, 3D PNIPAAm-PEG hydrogel, and 3D alginate hydrogel cultures) based on our previous reports. We expanded hPSCs and differentiated them into ECs in three culture systems. Both 3D hydrogel systems could mimic an in vivo physiologically relevant microenvironment to protect cells from shear force and prevent cell agglomeration, leading to a high culture efficiency and a high volumetric yield. We demonstrated that hPSC-ECs produced from both hydrogel systems had similar results as 2D-ECs. The transcriptome analysis showed that PEG-ECs and alginate-ECs displayed a functional phenotype due to their higher gene expressions in vasculature development, extracellular matrix, angiogenesis, and glycolysis, while 2D-ECs showed a proliferative phenotype due to their higher gene expressions in cell proliferation. Taken together, both PEG- and alginate-hydrogel systems will significantly advance the applications of hPSC-ECs in various biomedical fields

Analysis on WeChat-Based Blended Learning in Network Marketing Course

Blended learning is an information-based teaching method. This method is applied in network marketing course and verifying its teaching effect is a problem worthy of research. By combining blended learning-related theories, student features, and course requirements, a WeChat-based blended learning pattern applicable to network marketing course was proposed in this study. A teaching design of WeChat-based blended learning was created, followed by a teaching experiment. Quantitative analysis was used to verify the teaching results of the experimental and control classes. Results demonstrate that the learning effect of experimental class adopting WeChat-based blended learning is superior to that of the control class adopting traditional teaching method. WeChat-based blended learning lengthens extracurricular learning time, enhances students’ learning interest, and increases the opportunity of student–teacher exchange. This study provides references for developing WeChat-based hybrid learning

Virtual Restoration of Stained Chinese Paintings Using Patch-Based Color Constrained Poisson Editing with Selected Hyperspectral Feature Bands

Stains, as one of most common degradations of paper cultural relics, not only affect paintings’ appearance, but sometimes even cover the text, patterns, and colors contained in the relics. Virtual restorations based on common red–green–blue images (RGB) which remove the degradations and then fill the lacuna regions with the image’s known parts with the inpainting technology could produce a visually plausible result. However, due to the lack of information inside the degradations, they always yield inconsistent structures when stains cover several color materials. To effectively remove the stains and restore the covered original contents of Chinese paintings, a novel method based on Poisson editing is proposed by exploiting the information inside the degradations of selected three feature bands as the auxiliary information to guide the restoration since the selected feature bands captured fewer stains and could expose the covered information. To make the Poisson editing suitable for stain removal, the feature bands were also exploited to search for the optimal patch for the pixels in the stain region, and the searched patch was used to construct the color constraint on the original Poisson editing to ensure the restoration of the original color of paintings. Specifically, this method mainly consists of two steps: feature band selection from hyperspectral data by establishing rules and reconstruction of stain contaminated regions of RGB image with color constrained Poisson editing. Four Chinese paintings (‘Fishing’, ‘Crane and Banana’, ‘the Hui Nationality Painting’, and ‘Lotus Pond and Wild Goose’) with different color materials were used to test the performance of the proposed method. Visual results show that this method can effectively remove or dilute the stains while restoring a painting’s original colors. By comparing values of restored pixels with nonstained pixels (reference of their same color materials), images processed by the proposed method had the lowest average root mean square error (RMSE), normalized absolute error (NAE), and average differences (AD), which indicates that it is an effective method to restore the stains of Chinese paintings

Multi-Level Support Technology and Application of Deep Roadway Surrounding Rock in the Suncun Coal Mine, China

To solve these problems of poor supporting effect and serious deformation and failure of surrounding rock of mining roadway under deep mining stress, a FLAC-3D numerical calculation model is established with −800 m level no. 2424 upper roadway in the Suncun Coal Mine as the background to compare the stress, deformation, and failure law of surrounding rock of mining roadway under once support and multi-level support with the same support strength. It is found that the multi-level support technology has obvious advantages in the surrounding rock of the horizontal roadway on the 2424 working face. From this, the key parameters of multi-level support are determined, and the field industrial test is carried out. The results show that the overall deformation of the surrounding rock is obviously reduced after multi-level support. The displacement of the two sides is reduced by about 40%, the displacement of the roof and floor is reduced by about 30%, and the plastic zone of the roadway is reduced by about 75%. The peak value of concentrated stress decreases from 98.7 MPa to 95.8 MPa, which decreases slightly. The integrity and stability of the surrounding rock are excellent, and the support effect is satisfactory. The research can provide reference and technical support for surrounding rock control of deep high-stress mining roadways

Creep Constitutive Model and Numerical Realization of Coal-Rock Combination Deteriorated by Immersion

Coal-rock combination refers to the coal and rock as a whole, and the failure of the whole structure of the combination is the main cause for the instability of the deep underground engineering. In deep underground engineering, the coal-rock combination is usually under certain hydrogeological conditions, and it is prone to seepage and rheological failure instability accidents due to the long-term action of water and stress. In this study, the creep constitutive model of coal-rock combination considering the influence of moisture content was established based on the Burgers creep model. According to the experimental results of triaxial creep of rock, the relationship between the moisture content and the parameter of the Burgers creep model was derived, and the correctness of the constitutive model in this study was verified. Then, through the C++ language, the core equation of the model was modified, and the numerical calculation of the model was realized by introducing the coal-rock combination creep model considering the influence of moisture content into FLAC3D numerical simulation software. Finally, the model was used to simulate and study the creep characteristics of coal-rock combination with different moisture contents under triaxial loading. The results showed that the stress environment and moisture content have significant effects on the creep characteristics of the coal-rock combination. Under the same stress state, with the increased of moisture content, the strain rate of the coal-rock combination exhibited a non-linear rapid increase in the constant-velocity creep stage, the limit creep deformation and the instantaneous elastic deformation increased, and the viscosity coefficient was significantly decreased. For example, when the axial stress was 5 MPa and the moisture content increased from 0% to 1.5%, the strain rate increased by 44.06%, the limit creep deformation increased by 20%, the instantaneous elastic deformation increased 10.53%, and the viscosity coefficient decreased by about 50%. When the moisture content is 0%, the axial stress increased from 5 to 14 MPa, and the limit creep deformation increased nearly four times. With the increase of moisture content, this value will further expand. The research conclusions can provide a certain reference basis for the long-term stability control of surrounding rock in underground engineering affected by the water