Prognosis signature for predicting the survival and immunotherapy response in esophageal carcinoma based on cellular senescence-related genes

BackgroundCellular senescence occurs throughout life and can play beneficial roles in a variety of physiological processes, including embryonic development, tissue repair, and tumor suppression. However, the relationship between cellular senescence-related genes (CSRGs) and immunotherapy in esophageal carcinoma (ECa) remains poorly defined.MethodsThe data set used in the analysis was retrieved from TCGA (Research Resource Identifier (RRID): SCR_003193), GEO (RRID: SCR_005012), and CellAge databases. Data processing, statistical analysis, and diagram formation were conducted in R software (RRID: SCR_001905) and GraphPad Prism (RRID: SCR_002798). Based on CSRGs, we used the TCGA database to construct a prognostic signature for ECa and then validated it in the GEO database. The predictive efficiency of the signature was evaluated using receiver operating characteristic (ROC) curves, Cox regression analysis, nomogram, and calibration curves. According to the median risk score derived from CSRGs, patients with ECa were divided into high- and low-risk groups. Immune infiltration and immunotherapy were also analyzed between the two risk groups. Finally, the hub genes of the differences between the two risk groups were identified by the STRING (RRID: SCR_005223) database and Cytoscape (RRID: SCR_003032) software.ResultsA six-gene risk signature (DEK, RUNX1, SMARCA4, SREBF1, TERT, and TOP1) was constructed in the TCGA database. Patients in the high-risk group had a worse overall survival (OS) was disclosed by survival analysis. As expected, the signature presented equally prognostic significance in the GSE53624 cohort. Next, the Area Under ROC Curve (AUC=0.854) and multivariate Cox regression analysis (HR=3.381, 2.073-5.514, P<0.001) also proved that the risk signature has a high predictive ability. Furthermore, we can more accurately predict the prognosis of patients with ECa by nomogram constructed by risk score. The result of the TIDE algorithm showed that ECa patients in the high-risk group had a greater possibility of immune escape. At last, a total of ten hub genes (APOA1, MUC5AC, GC, APOA4, AMBP, FABP1, APOA2, SOX2, MUC8, MUC17) between two risk groups with the highest interaction degrees were identified. By further analysis, four hub genes (APOA4, AMBP, FABP1, and APOA2) were related to the survival differences of ECa.ConclusionsOur study reveals comprehensive clues that a novel signature based on CSRGs may provide reliable prognosis prediction and insight into new therapy for patients with ECa

Research on vibration characteristics of marine power-spilt gear system

In order to carry out a more in-depth study on the vibration characteristics of the marine power-spilt gear system, a nonlinear dynamic model of a certain type of marine gearbox was established, taking into account factors such as dynamic backlash and time-varying mesh stiffness. The numerical simulation method was used to calculate the variation of the system vibration response with input speed, the torsional stiffness of the linkage shaft and the stiffness of the output shaft under different backlach. The results show that the system will occur resonance as the input speed increases; the torsional stiffness of the linkage axis has an optimal value, so that the vibration response amplitude of the system is the smallest; as the support stiffness of the output shaft increases, the vibration acceleration of the output shaft does not change much, while the vibration displacement is significantly reduced

A new method calculating load balance of sliding bearing by using neural network PID algorithm

Aiming at low efficiency of existing sliding bearing load balance calculation, a new method that based on neural network proportional-integral-derivative (PID) algorithm is proposed for the first time, in which a compound control algorithm combining neural network and PID algorithm is applied. In this new method, the load error is taken as the input of the system, and the eccentricity of the bearing is used as the input of the controller, and the output of the system is the oil film force of the bearing. Comparing with traditional method, calculation results show that: the number of iterations calculated by neural network PID algorithm is less than traditional one and has higher efficiency and stronger adaptability under different loads

Quantitative evaluation and models of hydrocarbon accumulation controlled by faults in the Pearl River Mouth Basin

The Pearl River Mouth Basin is the largest petroliferous basin in the northern South China Sea, where hydrocarbon accumulation is strongly controlled by fault activities. This study performed the quantitative evaluation of the effects of faults on hydrocarbon migration and accumulation in the basin. The results indicate that the critical values of vertical migration of middle-shallow hydrocarbon, including the active strength of faults and the ratio of fault throw to shale caprock thickness, were up to 10 m/Ma and 5, respectively. The lateral hydrocarbon migration efficiency of the unbreached relay zone was higher than that of the barely breached and strongly breached types. The lower critical value of shale gouge ratio for the clay sealing efficiency was 0.32. Additionally, the zones with the EW-trending transtensional faults were found to have unique dual functions of migration and stress sealing, suggesting that the linking fault positions play important roles in the lateral migration of hydrocarbons. Finally, seven hydrocarbon accumulation models controlled by faults in different tectonic settings were constructed to clarify the effects of faults on the vertical and lateral migrations of hydrocarbon. These models suggested that fine hydrocarbon exploration should be undertaken in the northeastern Baiyun Sag, and that middle-deep hydrocarbon exploration should be enhanced in the Enping, Huizhou, and southwestern Baiyun Sags.Cited as: Peng, G., Wu, Z., Dai, Y., Zhang, L., Yu, S., Wang, W., Pang, H. Quantitative evaluation and models of hydrocarbon accumulation controlled by faults in the Pearl River Mouth Basin. Advances in Geo-Energy Research, 2023, 8(2): 89-99. https://doi.org/10.46690/ager.2023.05.0

Effects of Superabsorbent Polymers on the Hydraulic Parameters and Water Retention Properties of Soil

Superabsorbent polymers (SAPs) are widely applied in dryland agriculture. However, their functional property of repeated absorption and release of soil water exerts periodic effects on the hydraulic parameters and water-retention properties of soil, and as this property gradually diminishes with time, its effects tend to be unstable. During the 120-day continuous soil cultivation experiment described in this paper, horizontal soil column infiltration and high-speed centrifugation tests were conducted on SAP-treated soil to measure unsaturated diffusivity D and soil water characteristic curves. The experimental results suggest that the SAP increased the water retaining capacity of soil sections where the suction pressure was between 0 and 3,000 cm. The SAP significantly obstructed water diffusion in the soil in the early days of the experiment, but the effect gradually decreased in the later period. The average decrease in water diffusivity in the treatment groups fell from 76.6% at 0 days to 1.2% at 120 days. This research also provided parameters of time-varying functions that describe the unsaturated diffusivity D and unsaturated hydraulic conductivity K of soils under the effects of SAPs; in future research, these functions can be used to construct water movement models applicable to SAP-treated soil

MicroRNA-7 inhibits epithelial-to-mesenchymal transition and metastasis of breast cancer cells via targeting FAK expression

10.1371/journal.pone.0041523PLoS ONE78

A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin

A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles (PD-MNPs) was prepared and characterized. The widely used Aspergillus niger lipase (ANL) was immobilized on the PD-MNPs (ANL@PD-MNPs) with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significantly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy. A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin (DMY) in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation

Distribution, occurrence characteristics and geological origin of typical hazardous elements in low-medium ash coal of Huainan coalfield

With the continuous development, processing and utilization of coal resources in our province, the existence of typical harmful elements in coal is bound to have a negative impact on the efficient and clean utilization of coal resources. Based on the mass data analysis of typical hazardous elements in coal of Huainan coalfield, taking the main coal seams (No.13-1, No.11-2, No.8, No.6, No.4 and No.1) as the research objects, the occurrence characteristics and geological genesis of typical hazardous elements were comprehensively analyzed by the means of ICP-MS, AFS, stepwise chemical extraction and cluster analysis. The results were as follows: ① Typical hazardous elements Cr, Co, Se and Pb in coal of Huainan coalfield are “lightly enriched”, Hg is “highly enriched” and other elements are in “normal range”; ② The high value area of As element content in Huainan coalfield was mainly located in the west of coalfield; the high value region of Hg element was located in the east of coalfield, followed by the west; the remaining eight elements (Cr, Mn, Co, Ni, Se, Cd, Sb and Pb) were all higher in coal of Panji mining area of Huainan coalfield (especially near Pansan coal mine). The late magmatic hydrothermal process in Panji-Zhuji region may be the main reason for the relative enrichment of hazardous elements such as Cr, Mn, Co, Ni, Se, Cd, Sb and Pb. ③ There were many ion-exchange States in the occurrence state of Hg, and the inorganic components brought by magmatic hydrothermal action may have little influence on the enrichment degree of Hg element. The magmatic hydrothermal intrusion in the late diagenesis period had no obvious influence on As, and the low content of As in coal may be related to the low content of As in coal-forming plants

Orbital-angular-momentum mode-group multiplexed transmission over a graded-index ring-core fiber based on receive diversity and maximal ratio combining

An orbital-angular-momentum (OAM) mode-group multiplexing (MGM) scheme using high-order mode groups (MGs) in a graded-index ring-core fiber (GIRCF) is proposed, in which a receive-diversity architecture is designed for each MG to suppress the mode partition noise resulting from random intra-group mode crosstalk. The signal-to-noise ratio (SNR) of the received signals is further improved by a simple maximal ratio combining (MRC) technique on the receiver side to efficiently take advantage of the diversity gain of the receiver. Intensity-modulated direct-detection (IM-DD) systems transmitting three OAM mode groups with total 100-Gb/s discrete multi-tone (DMT) signals over a 1-km GIRCF and two OAM mode groups with total 40-Gb/s DMT signals over an 18.4-km GIRCF are experimentally demonstrated, respectively, to confirm the feasibility of our proposed OAM-MGM scheme