FRAP Analysis: Accounting for Bleaching during Image Capture

The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.Molecular and Cellular Biolog

NOVEL SECURED INTER-PERSONAL AREA NETWORK GROUP MANAGEMENT IN LOW-POWER AND LOSSY NETWORKS

Low-power and Lossy Network (LLN) environments may comprise, possibly among other things, different personal area networks (PANs) resulting in, for example, node communication challenges across or between PANs. To address these types of challenges, techniques are presented herein that support a novel secure group management method to self-solve the inter-PAN problem that is both low-cost and customer-friendly. Aspects of the techniques presented herein encompass establishing a secure node-to-node (N2N) communication link between involved inter-PAN nodes, automatically looking for the relay neighbors between different PANs (as the inter-PAN node can help with forwarding the local the Routing Protocol for LLN (RPL) messages), automatically propagating the group information and maintaining the local RPL tree between the inter-PAN nodes, etc. Aspects of the techniques presented herein employ, among other things, spreading PAN advertisement (PA) messages with group and hop information to identify a feasible routing path, using the Extensible Authentication Protocol-Tunneled Transport Layer Security (EAP-TTLS) protocol to establish a secure transport tunnel, automatically unicasting a destination-oriented directed acyclic graph (DODAG) Information Solicitation (DIS) message to join the group tree, etc. Under aspects of the techniques presented herein an application server need not know the topology of a network

BLOCK ACKNOWLEDGEMENT MECHANISM FOR SPEEDING UP NODE-TO-NODE TRAFFIC IN LOW-POWER AND LOSSY NETWORKS

The Wireless Smart Utility Network (Wi-SUN) Alliance promotes interoperability for large scale wireless mesh networks (WMNs). Such Low-Power and Lossy Networks (LLNs) are widely used in industrial Internet of Things (IoT) settings. In order to improve throughput in node-to-node (N2N) communications for Wi-SUN based wireless nodes, techniques are presented herein that support the addition of a Block Acknowledgement (BA) mechanism to the Wi-SUN protocol. Such a mechanism may, among other things, eliminate a significant acknowledgement (ACK) confirmation overhead when a pair of nodes are using an Extended Directional Frame Exchange (EDFE) mode or an Adaptive Modulation (AM) mode to overcome radio interference

FLOW CONTROL USING BANDWIDTH ALLOCATION STRATEGY IN LOW-POWER AND LOSSY NETWORKS

Presented herein are techniques for reduce the risk of transmitting collision in wireless networks. The techniques presented herein use a deterministic bandwidth allocation strategy for a central device (central node). As a result, each neighbor can leverage its own schedule to transmit packets with low risk of collision. The central device spreads schedules via DODAG Information Object (DIO) messages in its neighborhood, while dynamically adjusting distribution, per round, according to predicted traffic of its neighbors. The portion of downward and guest account is determined from the software-defined networking (SDN) controller. The SDN controller can design appropriated ratio for each central device on-demand and configure them as a policy

A closure mechanism for screech coupling in rectangular twin jets

Twin-jet configuration allows two different scenarios to close the screech feedback. For each jet, there is one loop involving disturbances which originate in that jet and arrive at its own receptivity point in-phase (self-excitation). The other loop is associated with free-stream acoustic waves that radiate from the other jet, reinforcing the self-excited screech (cross-excitation). In this work, the role of the free-stream acoustic mode and the guided jet mode as a closure mechanism for twin rectangular jet screech is explored by identifying eligible points of return for each path, where upstream waves propagating from such a point arrive at the receptivity location with an appropriate phase relation. Screech tones generated by these jets are found to be intermittent with an out-of-phase coupling as a dominant coupling mode. Instantaneous phase difference between the twin jets computed by the Hilbert transform suggests that a competition between out-of-phase and in-phase coupling is responsible for the intermittency. To model wave components of the screech feedback while ensuring perfect phase-locking, an ensemble average of leading spectral proper orthogonal decomposition modes is obtained from several segments of large-eddy simulations data that correspond to periods of invariant phase difference between the two jets. Each mode is then extracted by retaining relevant wavenumber components produced via a streamwise Fourier transform. Spatial cross-correlation analysis of the resulting modes shows that most of the identified points of return for the cross-excitation are synchronised with the guided jet mode self-excitation, supporting that it is preferred in closing rectangular twin-jet screech coupling

(R)-1-[(S)-(3-Cyano­thio­morpholino)carbon­yl]-2-methyl­propyl­aminium chloride dihydrate

In the title compound, C10H18N3OS+·Cl−·2H2O, the three C atoms of the isopropyl group are disordered and were refined using a split-site mode [occupancy ratio 0.53 (3):0.47 (3)]. In the crystal, the cations, anions and water mol­ecules are connected via O—H⋯O, O—H⋯Cl, N—H⋯Cl and N—H⋯O hydrogen bonding

Reaching the last mile: best practices in leveraging the power of ICTs to communicate climate services to farmers at scale

This report reviews key ICTs for Development (ICT4D) Programs, Innovations and Information Exchange Platforms which are experimented within South Asia to explore the use and scale-ability of these innovative approaches to other parts of Africa and the developing world. Learning from the pioneering experiences of pilot projects across India and Africa in ICT development, we assess the potential ICTs offer to not only communicate climate information and related advisory services but also to build capacity and increase the resilience of rural smallholders. It is our hope that such South-South learning can pave the way for improved cross-regional experience sharing to tackle common challenges in reaching ‘the last mile’ with salient rural extension services, including climate information services