Crystal-Induced Inflammation: Studies of the Mechanism of Crystal-Membrane Interactions

Studies of the interactions of monosodium urate monohydrate (MSUM) crystals and calcium Pyrophosphate dihydrate triclinic (CPPD) crystals with biomembranes have been reviewed. Crystalmembrane binding and crystal-induced membranolysis have been studied using human erythrocytes as a model membrane system. The extent of MSUM-membrane binding was determined by incorporating a hydrophobic, fluorescent probe into the membranes, centrifugation to separate free membranes from membranes with bound crystals and quantitation of free membranes by measuring the total fluorescence intensity. The ability of MSUM and CPPD to hemolyse red cells was used as a measure of the membranolytic potential of the crystals. Fluorescence polarization studies showed that MSUM-membrane binding resulted in fluidization of the membrane. Cross-linking of the membrane proteins of the erythrocyte or the presence of divalent cations in the incubation medium inhibited MSUM induced hemolysis. These findings were explained by hypothesizing a pore model mechanism for MSUM induced membranolysis as follows. Binding of crystals to membranes induces the redistribution of transmembrane proteins into clusters or aggregates leading to pore formation. The pores permit the leakage of low molecular weight soluble compounds and ions across the membrane which is followed by osmotic rupture of the membran

A Dynamic Programming Approach to Adaptive Fractionation

We conduct a theoretical study of various solution methods for the adaptive fractionation problem. The two messages of this paper are: (i) dynamic programming (DP) is a useful framework for adaptive radiation therapy, particularly adaptive fractionation, because it allows us to assess how close to optimal different methods are, and (ii) heuristic methods proposed in this paper are near-optimal, and therefore, can be used to evaluate the best possible benefit of using an adaptive fraction size. The essence of adaptive fractionation is to increase the fraction size when the tumor and organ-at-risk (OAR) are far apart (a "favorable" anatomy) and to decrease the fraction size when they are close together. Given that a fixed prescribed dose must be delivered to the tumor over the course of the treatment, such an approach results in a lower cumulative dose to the OAR when compared to that resulting from standard fractionation. We first establish a benchmark by using the DP algorithm to solve the problem exactly. In this case, we characterize the structure of an optimal policy, which provides guidance for our choice of heuristics. We develop two intuitive, numerically near-optimal heuristic policies, which could be used for more complex, high-dimensional problems. Furthermore, one of the heuristics requires only a statistic of the motion probability distribution, making it a reasonable method for use in a realistic setting. Numerically, we find that the amount of decrease in dose to the OAR can vary significantly (5 - 85%) depending on the amount of motion in the anatomy, the number of fractions, and the range of fraction sizes allowed. In general, the decrease in dose to the OAR is more pronounced when: (i) we have a high probability of large tumor-OAR distances, (ii) we use many fractions (as in a hyper-fractionated setting), and (iii) we allow large daily fraction size deviations.Comment: 17 pages, 4 figures, 1 tabl

Record Maximum Oscillation Frequency in C-face Epitaxial Graphene Transistors

The maximum oscillation frequency (fmax) quantifies the practical upper bound for useful circuit operation. We report here an fmax of 70 GHz in transistors using epitaxial graphene grown on the C-face of SiC. This is a significant improvement over Si-face epitaxial graphene used in the prior high frequency transistor studies, exemplifying the superior electronics potential of C-face epitaxial graphene. Careful transistor design using a high {\kappa} dielectric T-gate and self-aligned contacts, further contributed to the record-breaking fmax

Ultrafast Hole Trapping and Relaxation Dynamics in p-Type CuS Nanodisks

CuS nanocrystals are potential materials for developing low-cost solar energy conversion devices. Understanding the underlying dynamics of photoinduced carriers in CuS nanocrystals is essential to improve their performance in these devices. In this work, we investigated the photoinduced hole dynamics in CuS nanodisks (NDs) using the combination of transient optical (OTA) and X-ray (XTA) absorption spectroscopy. OTA results show that the broad transient absorption in the visible region is attributed to the photoinduced hot and trapped holes. The hole trapping process occurs on a subpicosecond time scale, followed by carrier recombination (~100 ps). The nature of the hole trapping sites, revealed by XTA, is characteristic of S or organic ligands on the surface of CuS NDs. These results not only suggest the possibility to control the hole dynamics by tuning the surface chemistry of CuS but also represent the first time observation of hole dynamics in semiconductor nanocrystals using XTA

The Dwarf Novae of Shortest Period

We present observations of the dwarf novae GW Lib, V844 Her, and DI UMa. Radial velocities of H-alph yield orbital periods of 0.05332 +- 0.00002 d (= 76.78 m) for GW Lib and and 0.054643 +- 0.000007 d (= 78.69 m) for V844 Her. Recently, the orbital period of DI UMa was found to be only 0.054564 +- 0.000002 d (= 78.57 m) by Fried et al. (1999), so these are the three shortest orbital periods among dwarf novae with normal-abundance secondaries. GW Lib has attracted attention as a cataclysmic binary showing apparent ZZ Ceti-type pulsations of the white dwarf primary. Its spectrum shows sharp Balmer emission flanked by strong, broad Balmer absorption, indicating a dominant contribution by white-dwarf light. Analysis of the Balmer absorption profiles is complicated by the unknown residual accretion luminosity and lack of coverage of the high Balmer lines. Our best-fit model atmospheres are marginally hotter than the ZZ Ceti instability strip, in rough agreement with recent ultraviolet results from HST. The spectrum and outburst behavior of GW Lib make it a near twin of WZ Sge, and we estimate it to have a quiescent V absolute magnitude 12. Comparison with archival data reveals proper motion of 65 +- 12 mas/yr. The mean spectrum of V844 Her is typical of SU UMa dwarf novae. We detected superhumps in the 1997 May superoutburst with superhump period = 0.05597 +- 0.00005 d. The spectrum of DI UMa appears normal for a dwarf nova near minimum light. These three dwarf novae have nearly identical short periods but completely dissimilar outburst characteristics. We discuss possible implications.Comment: Accepted for publication in Publications of the Astronomical Society of the Pacific; 16 pages, 6 figure

PCA-based lung motion model

Organ motion induced by respiration may cause clinically significant targeting errors and greatly degrade the effectiveness of conformal radiotherapy. It is therefore crucial to be able to model respiratory motion accurately. A recently proposed lung motion model based on principal component analysis (PCA) has been shown to be promising on a few patients. However, there is still a need to understand the underlying reason why it works. In this paper, we present a much deeper and detailed analysis of the PCA-based lung motion model. We provide the theoretical justification of the effectiveness of PCA in modeling lung motion. We also prove that under certain conditions, the PCA motion model is equivalent to 5D motion model, which is based on physiology and anatomy of the lung. The modeling power of PCA model was tested on clinical data and the average 3D error was found to be below 1 mm.Comment: 4 pages, 1 figure. submitted to International Conference on the use of Computers in Radiation Therapy 201

T-Violation in K+→μ+νγK^+ \to \mu^+ \nu \gamma Decay And Supersymmetry

Measurement of the transverse muon polarization $P^{\bot}_{\mu}$ in the $K^+ \rightarrow \mu^+\nu\gamma$ decay will be attempted for the first time at the ongoing KEK E246 experiment and also at a proposed BNL experiment. We provide a general analysis of how $P^{\bot}_{\mu}$ is sensitive to the physical $CP$-violating phases in new physics induced four-Fermi interactions, and then we calculate the dominant contributions to $P^{\bot}_{\mu}$ from squark family mixings in generic supersymmetric models. Estimates of the upper bounds on $P^{\bot}_{\mu}$ are also given. It is found that a supersymmetry-induced right-handed quark current from $W$ boson exchange gives an upper limit on $P^{\bot}_{\mu}$ as large as a few per cent, whereas with charged-Higgs-exchange induced pseudoscalar interaction, $P^{\bot}_{\mu}$ is no larger than a few tenths of a per cent. Possible correlations between the muon polarization measurements in $K^+ \rightarrow \mu^+\nu\gamma$ and $K^+ \rightarrow \pi^0\mu^+\nu$ decays are discussed, and distinctive patterns of this correlation from squark family-mixings and from the three-Higgs-doublet model are noted.Comment: Revtex, 29 pages including 4 epsf figure

Fragment screening targeting Ebola virus nucleoprotein C-terminal domain identifies lead candidates

The Ebola Virus is a causative agent of viral hemorrhagic fever outbreaks and a potential global health risk. The outbreak in West Africa (2013-2016) led to 11,000+ deaths and 30,000+ Ebola infected individuals. The current outbreak in the Democratic Republic of Congo (DRC) with 3000+ confirmed cases and 2000+ deaths attributed to Ebola virus infections provides a reminder that innovative countermeasures are still needed. Ebola virus encodes 7 open reading frames (ORFs). Of these, the nucleocapsid protein (eNP) encoded by the first ORF plays many significant roles, including a role in viral RNA synthesis. Here we describe efforts to target the C-terminal domain of eNP (eNP-CTD) that contains highly conserved residues 641-739 as a pan-Ebola antiviral target. Interactions of eNP-CTD with Ebola Viral Protein 30 (eVP30) and Viral Protein 40 (eVP40) have been shown to be crucial for viral RNA synthesis, virion formation, and virion transport. We used nuclear magnetic response (NMR)-based methods to screened the eNP-CTD against a fragment library. Perturbations of 1

Theory of High-Tc Superconductivity: Accurate Predictions of Tc

The superconducting transition temperatures of high-Tc compounds based on copper, iron, ruthenium and certain organic molecules are discovered to be dependent on bond lengths, ionic valences, and Coulomb coupling between electronic bands in adjacent, spatially separated layers [1]. Optimal transition temperature, denoted as T_c0, is given by the universal expression $k_BT_c0 = e^2 \Lambda / \ell\zeta$; $\ell$ is the spacing between interacting charges within the layers, \zeta is the distance between interacting layers and \Lambda is a universal constant, equal to about twice the reduced electron Compton wavelength (suggesting that Compton scattering plays a role in pairing). Non-optimum compounds in which sample degradation is evident typically exhibit Tc < T_c0. For the 31+ optimum compounds tested, the theoretical and experimental T_c0 agree statistically to within +/- 1.4 K. The elemental high Tc building block comprises two adjacent and spatially separated charge layers; the factor e^2/\zeta arises from Coulomb forces between them. The theoretical charge structure representing a room-temperature superconductor is also presented.Comment: 7 pages 5 references, 6 figures 1 tabl