Intense CIII] 1907,1909 emission from a strong Lyman continuum emitting galaxy

We have obtained the first complete ultraviolet (UV) spectrum of a strong Lyman continuum(LyC) emitter at low redshift -- the compact, low-metallicity, star-forming galaxy J1154+2443 -- with a Lyman continuum escape fraction of 46% discovered recently. The Space Telescope Imaging Spectrograph spectrum shows strong Lya and CIII] 1909 emission, as well as OIII] 1666. Our observations show that strong LyC emitters can have UV emission lines with a high equivalent width (e.g. EW(CIII])$=11.7 \pm 2.9 \AA$ rest-frame), although their equivalent widths should be reduced due to the loss of ionizing photons. The intrinsic ionizing photon production efficiency of J1154+2443 is high, $\log(\xi_{\rm ion}^0)=25.56$ erg$^{-1}$ Hz, comparable to that of other recently discovered $z \sim 0.3-0.4$ LyC emitters. Combining our measurements and earlier determinations from the literature, we find a trend of increasing $\xi_{\rm ion}^0$ with increasing CIII] 1909 equivalent width, which can be understood by a combination of decreasing stellar population age and metallicity. Simple ionization and density-bounded photoionization models can explain the main observational features including the UV spectrum of J1154+2443.Comment: 5 pages, 4 figures. Accepted for publication in A&A Letter

Do galaxies that leak ionizing photons have extreme outflows?

To reionize the early universe, high-energy photons must escape the galaxies that produce them. It has been suggested that stellar feedback drives galactic outflows out of star-forming regions, creating low density channels through which ionizing photons escape into the inter-galactic medium. We compare the galactic outflow properties of confirmed Lyman continuum (LyC) leaking galaxies to a control sample of nearby star-forming galaxies to explore whether the outflows from leakers are extreme as compared to the control sample. We use data from the Cosmic Origins Spectrograph on the Hubble Space Telescope to measure the equivalent widths and velocities of Si II and Si III absorption lines, tracing neutral and ionized galactic outflows. We find that the Si II and Si III equivalent widths of the LyC leakers reside on the low-end of the trend established by the control sample. The leakers' velocities are not statistically different than the control sample, but their absorption line profiles have a different asymmetry: their central velocities are closer to their maximum velocities. The outflow kinematics and equivalent widths are consistent with the scaling relations between outflow properties and host galaxy properties -- most notably metallicity -- defined by the control sample. Additionally, we use the Ly\alpha\ profiles to show that the Si II equivalent width scales with the Ly\alpha\ peak velocity separation. We determine that the low equivalent widths of the leakers are likely driven by low metallicities and low H I column densities, consistent with a density-bounded ionization region, although we cannot rule out significant variations in covering fraction. While we do not find that the LyC leakers have extreme outflow velocities, the low maximum-to-central velocity ratios demonstrate the importance of the acceleration and density profiles for LyC and Ly\alpha\ escape. [abridged]Comment: 17 pages, 8 Figures. Accepted for publication in Astronomy & Astrophysic

A stringent yeast two-hybrid matrix screening approach for protein-protein interaction discovery

The yeast two-hybrid (Y2H) system is currently one of the most important techniques for protein-protein interaction (PPI) discovery. Here, we describe a stringent three-step Y2H matrix interaction approach that is suitable for systematic PPI screening on a proteome scale. We start with the identification and elimination of autoactivating strains that would lead to false-positive signals and prevent the identification of interactions. Nonautoactivating strains are used for the primary PPI screen that is carried out in quadruplicate with arrayed preys. Interacting pairs of baits and preys are identified in a pairwise retest step. Only PPI pairs that pass the retest step are regarded as potentially biologically relevant interactions and are considered for further analysis

J1154+2443: a low-redshift compact star-forming galaxy with a 46 per cent leakage of Lyman continuum photons

We report the detection of the Lyman continuum (LyC) radiation of the compact star-forming galaxy (SFG) J1154+2443 observed with the Cosmic Origins Spectrograph (COS) onboard the Hubble Space Telescope. This galaxy, at a redshift of z=0.3690, is characterized by a high emission-line flux ratio O32=[OIII]5007/[OII]3727=11.5. The escape fraction of the LyC radiation fesc(LyC) in this galaxy is 46 per cent, the highest value found so far in low-redshift SFGs and one of the highest values found in galaxies at any redshift. The narrow double-peaked Lya emission line is detected in the spectrum of J1154+2443 with a separation between the peaks Vsep of 199 km/s, one of the lowest known for Lya-emitting galaxies, implying a high fesc(Lya). Comparing the extinction-corrected Lya/Hb flux ratio with the case B value we find fesc(Lya) = 98 per cent. Our observations, combined with previous detections in the literature, reveal an increase of O32 with increasing fesc(LyC). We also find a tight anticorrelation between fesc(LyC) and Vsep. The surface brightness profile derived from the COS acquisition image reveals a bright star-forming region in the centre and an exponential disc in the outskirts with a disc scale length alpha=1.09 kpc. J1154+2443, compared to other known low-redshift LyC leakers, is characterized by the lowest metallicity, 12+logO/H=7.65+/-0.01, the lowest stellar mass M*=10^8.20 Msun, a similar star formation rate SFR=18.9 Msun/yr and a high specific SFR of 1.2x10^-7 yr^-1.Comment: 16 pages, 8 figures, accepted for publication in MNRAS. arXiv admin note: text overlap with arXiv:1605.0516