The stability of food intake between adolescence and adulthood: a 21-year follow-up

Studies of the diet of adolescents in the UK demonstrate that dietary habits known to be detrimental to health in adulthood are evident at an early age. For example, Gregory et al (2000) found 4-18 year olds in the UK to have a frequent consumption of fatty and sugary foods and low consumption of fruit and vegetables. Concerns have therefore been expressed regarding the diet of children and adolescents and the continuation of these dietary habits into adulthood (HEA, 1995; Gaziano, 1998). This study aimed to investigate the extent to which these concerns may be justified by determining the stability of food intake of a group of adolescents followed up 21 years later in adulthood. The investigation involved 202 individuals from whom dietary data were collected in 1979-80 (mean age 11.6 years) (Hackett et al. 1984) and again in 2000-1 (mean age 32.5 years). Dietary data were collected at both time-points using two 3 d estimated food diaries followed by an interview to determine portion sizes using the method considered most appropriate at the time, i.e. calibrated food models in 1979-80 and a photographic food atlas (Nelson et al. 1997) in 2000-1. Foods consumed were allocated to one, or a combination of, the five food groups of the ‘Balance of Good Health’ food selection guide (HEA, 1994) according to Gatenby et al. (1995). The weight of food eaten from each of the five food groups was calculated (percentage of total weight of food consumed) and Pearson correlation coefficients generated to provide an estimate of the stability of food intake. The HEA guide advises that a balanced diet should consist of around 33% fruit and vegetables, 33% bread, other cereals and potatoes, 8% foods containing fat and/or sugar, 12% meat, fish and alternatives and 15% milk and dairy products (Gatenby et al. 1995). A shift in the group’s food intake towards the recommendations had occurred with age, most notably with a decrease in foods containing fat and/or sugar and an increase in fruit and vegetables. Nevertheless, at both ages, intakes of foods containing fat and/or sugar, meat, fish and alternatives were higher, and fruit, vegetables, bread, other cereals and potatoes lower than currently recommended. In addition, although there was significant evidence of tracking of relative intake of bread, cereals and potatoes (P<0.01), fruit and vegetables (P<0.01), and meat, fish and alternatives (P=0.02) between 11.6 and 32.5 years, the correlations were not strong. In conclusion, food intake patterns had changed considerably from early adolescence through to adulthood in a direction more in line with the current recommendations. The predictive value of an adolescent’s food intake of their intake in adulthood was found to be significant, but not strong. Further investigations will consider the extent to which this is influenced by factors such as social class, gender and educational level, as well as assessing tracking in terms of relative nutrient intakes

Directionality in protein fold prediction

<p>Abstract</p> <p>Background</p> <p>Ever since the ground-breaking work of Anfinsen et al. in which a denatured protein was found to refold to its native state, it has been frequently stated by the protein fold prediction community that all the information required for protein folding lies in the amino acid sequence. Recent in vitro experiments and in silico computational studies, however, have shown that cotranslation may affect the folding pathway of some proteins, especially those of ancient folds. In this paper aspects of cotranslational folding have been incorporated into a protein structure prediction algorithm by adapting the Rosetta program to fold proteins as the nascent chain elongates. This makes it possible to conduct a pairwise comparison of folding accuracy, by comparing folds created sequentially from each end of the protein.</p> <p>Results</p> <p>A single main result emerged: in 94% of proteins analyzed, following the sense of translation, from N-terminus to C-terminus, produced better predictions than following the reverse sense of translation, from the C-terminus to N-terminus. Two secondary results emerged. First, this superiority of N-terminus to C-terminus folding was more marked for proteins showing stronger evidence of cotranslation and second, an algorithm following the sense of translation produced predictions comparable to, and occasionally better than, Rosetta.</p> <p>Conclusions</p> <p>There is a directionality effect in protein fold prediction. At present, prediction methods appear to be too noisy to take advantage of this effect; as techniques refine, it may be possible to draw benefit from a sequential approach to protein fold prediction.</p

Benchmarking Substellar Evolutionary Models Using New Age Estimates for HD 4747 B and HD 19467 B

Constraining substellar evolutionary models (SSEMs) is particularly difficult due to a degeneracy between the mass, age, and luminosity of a brown dwarf. In cases where a brown dwarf is found as a directly imaged companion to a star, as in HD 4747 and HD 19467, the mass, age, and luminosity of the brown dwarf are determined independently, making them ideal objects to use to benchmark SSEMs. Using the Center for High Angular Resolution Astronomy Array, we measured the angular diameters and calculated the radii of the host stars HD 4747 A and HD 19467 A. After fitting their parameters to the Dartmouth Stellar Evolution Database, MESA Isochrones and Stellar Tracks, and Yonsei-Yale isochronal models, we adopt age estimates of $10.74^{+6.75}_{-6.87}$ Gyr for HD 4747 A and $10.06^{+1.16}_{-0.82}$ Gyr for HD 19467 A. Assuming the brown dwarf companions HD 4747 B and HD 19467 B have the same ages as their host stars, we show that many of the SSEMs under-predict bolometric luminosities by $\sim$ 0.75 dex for HD 4747 B and $\sim 0.5$ dex for HD 19467 B. The discrepancies in luminosity correspond to over-predictions of the masses by $\sim$ 12\% for HD 4747 B and $\sim$ 30\% for HD 19467 B. We also show that SSEMs that take into account the effect of clouds reduce the under-prediction of luminosity to $\sim 0.6$ dex and the over-prediction of mass to $\sim 8\%$ for HD 4747 B, an L/T transition object that is cool enough to begin forming clouds. One possible explanation for the remaining discrepancies is missing physics in the models, such as the inclusion of metallicity effects.Comment: 12 pages, 6 figures, 5 tables, accepted to Ap

Assessing direct contributions of morphological awareness and prosodic sensitivity to children’s word reading and reading comprehension

We examined the independent contributions of prosodic sensitivity and morphological awareness to word reading, text reading accuracy, and reading comprehension. We did so in a longitudinal study of English-speaking children (N = 70). At 5 to 7 years of age, children completed the metalinguistic measures along with control measures of phonological awareness and vocabulary. Children completed the reading measures two years later. Morphological awareness, but not prosodic sensitivity made a significant independent contribution to word reading, text reading accuracy and reading comprehension. The effects of morphological awareness on reading comprehension remained after controls for word reading. These results suggest that morphological awareness needs to be considered seriously in models of reading development and that prosodic sensitivity might have primarily indirect relations to reading outcomes. Keywords: Morphological Awareness; Prosody; Word Reading; Reading Comprehension

The TRENDS High-contrast Imaging Survey. VIII. Compendium of Benchmark Objects

The physical properties of faint stellar and substellar objects often rely on indirect, model-dependent estimates. For example, the masses of brown dwarfs are usually inferred using evolutionary models, which are age dependent and have yet to be properly calibrated. With the goal of identifying new benchmark objects to test low-mass stellar and substellar models, we have carried out a comprehensive adaptive optics survey as part of the TaRgetting bENchmark-objects with the Doppler Spectroscopy high-contrast imaging program. Using legacy radial velocity measurements from the High Resolution Echelle Spectrometer at Keck, we have identified several dozen stars that show long-term Doppler accelerations. We present follow-up high-contrast observations from the campaign and report the discovery of 31 comoving companions, as well as 11 strong candidate companions, to solar-type stars with well-determined parallax and metallicity values. Benchmark objects of this nature lend themselves to orbit determinations, dynamical mass estimates, and independent compositional assessment. This compendium of benchmark objects will serve as a convenient test group to substantiate theoretical evolutionary and atmospheric models near the hydrogen fusing limit

The TRENDS High-contrast Imaging Survey. VIII. Compendium of Benchmark Objects

The physical properties of faint stellar and substellar objects often rely on indirect, model-dependent estimates. For example, the masses of brown dwarfs are usually inferred using evolutionary models, which are age dependent and have yet to be properly calibrated. With the goal of identifying new benchmark objects to test low-mass stellar and substellar models, we have carried out a comprehensive adaptive optics survey as part of the TaRgetting bENchmark-objects with the Doppler Spectroscopy high-contrast imaging program. Using legacy radial velocity measurements from the High Resolution Echelle Spectrometer at Keck, we have identified several dozen stars that show long-term Doppler accelerations. We present follow-up high-contrast observations from the campaign and report the discovery of 31 comoving companions, as well as 11 strong candidate companions, to solar-type stars with well-determined parallax and metallicity values. Benchmark objects of this nature lend themselves to orbit determinations, dynamical mass estimates, and independent compositional assessment. This compendium of benchmark objects will serve as a convenient test group to substantiate theoretical evolutionary and atmospheric models near the hydrogen fusing limit

Risk assessment for hospital admission in patients with COPD; a multi-centre UK prospective observational study.

In chronic obstructive pulmonary disease (COPD), acute exacerbation of COPD requiring hospital admission is associated with mortality and healthcare costs. The ERICA study assessed multiple clinical measures in people with COPD, including the short physical performance battery (SPPB), a simple test of physical function with 3 components (gait speed, balance and sit-to-stand). We tested the hypothesis that SPPB score would relate to risk of hospital admissions and length of hospital stay. Data were analysed from 714 of the total 729 participants (434 men and 280 women) with COPD. Data from this prospective observational longitudinal study were obtained from 4 secondary and 1 tertiary centres from England, Scotland, and Wales. The main outcome measures were to estimate the risk of hospitalisation with acute exacerbation of COPD (AECOPD and length of hospital stay derived from hospital episode statistics (HES). In total, 291 of 714 individuals experienced 762 hospitalised AECOPD during five-year follow up. Poorer performance of SPPB was associated with both higher rate (IRR 1.08 per 1 point decrease, 95% CI 1.01 to 1.14) and increased length of stay (IRR 1.18 per 1 point decrease, 95% CI 1.10 to 1.27) for hospitalised AECOPD. For the individual sit-to-stand component of the SPPB, the association was even stronger (IRR 1.14, 95% CI 1.02 to 1.26 for rate and IRR 1.32, 95% CI 1.16 to 1.49 for length of stay for hospitalised AECOPD). The SPPB, and in particular the sit-to-stand component can both evaluate the risk of H-AECOPD and length of hospital stay in COPD. The SPPB can aid in clinical decision making and when prioritising healthcare resources

C5a impairs phagosomal maturation in the neutrophil through phosphoproteomic remodeling.

Critical illness is accompanied by the release of large amounts of the anaphylotoxin, C5a. C5a suppresses antimicrobial functions of neutrophils which is associated with adverse outcomes. The signaling pathways that mediate C5a-induced neutrophil dysfunction are incompletely understood. Healthy donor neutrophils exposed to purified C5a demonstrated a prolonged defect (7 hours) in phagocytosis of Staphylococcus aureus. Phosphoproteomic profiling of 2712 phosphoproteins identified persistent C5a signaling and selective impairment of phagosomal protein phosphorylation on exposure to S. aureus. Notable proteins included early endosomal marker ZFYVE16 and V-ATPase proton channel component ATPV1G1. An assay of phagosomal acidification demonstrated C5a-induced impairment of phagosomal acidification, which was recapitulated in neutrophils from critically ill patients. Examination of the C5a-impaired protein phosphorylation indicated a role for the PI3K VPS34 in phagosomal maturation. Inhibition of VPS34 impaired neutrophil phagosomal acidification and killing of S. aureus. This study provides a phosphoproteomic assessment of human neutrophil signaling in response to S. aureus and its disruption by C5a, identifying a defect in phagosomal maturation and mechanisms of immune failure in critical illness.AJTW was a Gates Cambridge Scholar supported by the Gates Cambridge Trust from 2015-2018. ACM is supported by a Clinical Research Career Development Fellowship from the Wellcome Trust (WT 2055214/Z/16/Z). Grants to ACM from the Academy of Medical Sciences and European Society for Intensive Care Medicine supported this work. The study was also supported by the National Institute for Health Research (NIHR) Newcastle Biomedical Research Centre (IS-BRC-1215-20001) and the Medical Research Council SHIELD antimicrobial resistance consortium (MR/ N02995X/1

High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source

High-resolution microcomputed tomography with benchtop X-ray sources requires long scan times because of the heat load limitation on the anode. We present an alternative, high-brightness plasma-based X-ray source that does not suffer from this restriction. A demonstration of tomography of a centimeter-scale complex organism achieves equivalent quality to a commercial scanner. We will soon be able to record such scans in minutes, rather than the hours required by conventional X-ray tubes