Damage monitoring in fibre reinforced mortar by combined digital image correlation and acoustic emission

International audienceThe present work aims at developing a methodology for the detection and monitoring of damage and fractures in building materials in the prospects of energetic renovation. Digital image correlation (DIC) and acoustic emission (AE) monitoring were simultaneously performed during tensile loading tests of fibre reinforced mortar samples. The full-field displacement mappings obtained by DIC revealed all ranges of cracks, from microscopic to macroscopic, and an image processing procedure was conducted as to quantify their evolution in the course of the degradation of the samples. The comparison of these measurements with the acoustic activity of the material showed a fair match in terms of quantification and localisation of damage. It is shown that after such a calibration procedure, AE monitoring can be autonomously used for the characterisation of damage and fractures at larger scales

Contribution to the physically anchored linkage map of the pig

2 tables 1 graph. Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-AccessInternational audienc

An overview of gene expression dynamics during early ovarian folliculogenesis: specificity of follicular compartments and bi-directional dialog

Chantier qualité GABackground: Successful early folliculogenesis is crucial for female reproductive function. It requires appropriate gene specific expression of the different types of ovarian cells at different developmental stages. To date, most gene expression studies on the ovary were conducted in rodents and did not distinguish the type of cell. In mono-ovulating species, few studies have addressed gene expression profiles and mainly concerned human oocytes. Results: We used a laser capture microdissection method combined with RNA-seq technology to explore the transcriptome in oocytes and granulosa cells (GCs) during development of the sheep ovarian follicle. We first documented the expression profile of 15 349 genes, then focused on the 5 129 genes showing differential expression between oocytes and GCs. Enriched functional categories such as oocyte meiotic arrest and GC steroid synthesis reflect two distinct cell fates. We identified the implication of GC signal transduction pathways such as SHH, WNT and RHO GTPase. In addition, signaling pathways (VEGF, NOTCH, IGF1, etc.) and GC transzonal projections suggest the existence of complex cell-cell interactions. Finally, we highlighted several transcription regulators and specifically expressed genes that likely play an important role in early folliculogenesis. Conclusions: To our knowledge, this is the first comprehensive exploration of transcriptomes derived from in vivo oocytes and GCs at key stages in early follicular development in sheep. Collectively, our data advance our understanding of early folliculogenesis in mono-ovulating species and will be a valuable resource for unraveling human ovarian dysfunction such as premature ovarian failure (POF)

Towards the identification of RN gene involved in meat quality in pigs

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Chromosome 7 mapping of a quantitative trait locus for fat androstenone level in Meishan X Large White F2 entire male pigs

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Mapping quantitative trait loci in a Meishan X Large White F2 population

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Current status of QTL detection in large white X Meishan crosses in France

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Genome-Wide Identification of the Mutation Underlying Fleece Variation and Discriminating Ancestral Hairy Species from Modern Woolly Sheep

The composition and structure of fleece variation observed in mammals is a consequence of a strong selective pressure for fiber production after domestication. In sheep, fleece variation discriminates ancestral species carrying a long and hairy fleece from modern domestic sheep (Ovis aries) owning a short and woolly fleece. Here, we report that the "woolly" allele results from the insertion of an antisense EIF2S2 retrogene (called asEIF2S2) into the 3' UTR of the IRF2BP2 gene leading to an abnormal IRF2BP2 transcript. We provide evidence that this chimeric IRF2BP2/asEIF2S2 messenger 1) targets the genuine sense EIF2S2 RNA and 2) creates a long endogenous double-stranded RNA which alters the expression of both EIF2S2 and IRF2BP2mRNA. This represents a unique example of a phenotype arising via a RNA-RNA hybrid, itself generated through a retroposition mechanism. Our results bring new insights on the sheep population history thanks to the identification of the molecular origin of an evolutionary phenotypic variatio