210 research outputs found

    Europese miljoenen voor stamcelonderzoek bij planten

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    Onderzoek levert fundamentele kennis over plantengroei, relevant voor de landbouw

    Low-fat and still creamy

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    Cheese specialist Zijerveld wanted its range of cheeses to include a tasty goat’s cheese with only 30 percent fat. Nobody had managed that using traditional techniques. Wageningen UR Food & Biobased Research came up with a new formula. Healthier and just as tasty

    Vetarm en toch lekker romig

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    Kaasspecialist Zijerveld wilde een smaakvolle geitenkaas met slechts 30 procent vet in het assortiment. Met ambachtelijke technieken lukte dat niet. Wageningen UR Food & Biobased Research vond een nieuwe receptuur voor het kaasje. Gezonder en net zo lekker

    Lang zal ze leven

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    Melkkoeien gaan na een jaar of zes naar de slager. Gezondheidsproblemen zijn vaak een reden om ze af te voeren, maar met de juiste aanpak kunnen ze zeker twee jaar langer mee. Dat is goed voor het koeienwelzijn en levert geld Ă©n milieuvoordelen op

    Kruiden verdringen antibiotica

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    Vanwege beperking van het antibioticagebruik vallen veehouders steeds vaker terug op kruiden, bacteriedrankjes en andere natuurlijke middelen om dieren gezond te maken Ă©n te houden. Hoewel harde medische claims niet altijd voorhanden zijn, boeken boeren soms spectaculaire resultaten met natuurlijke preparaten

    Letter from the Citizens of Ommen to the Governor of Overijssel

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    Sixteen citizens of Ommen sent a communication to the Governor of the Province that they saw no need for him to send a military detachment to Ommen now that the disturbances had ended. Besides, a relatively few people were the troublemakers. The billeting of military people will be a great burden to the citizens of Ommen.https://digitalcommons.hope.edu/vrp_1830s/1048/thumbnail.jp

    A comparative study of the electrochemical properties of vitamin B-6 related compounds at physiological pH

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    A comparative study of vitamin B6 group and related compounds in buffered solutions using electrochemical techniques has been performed at neutral pH. Irreversible bi- or tetra-electronic processes are observed for these substances, and the electron transfer coefficient (αn) calculated. It was concluded that either the first or second electron transfer were the rate determining step of the electrode process. The diffusion coefficient of these substances was calculated and the values given follow an inverse tendency to the molecular size. For aldehydes the values obtained were corrected of the hydration reaction. It is important to remark that catalytic waves were reported for the first time for these compounds. Using a model involving the nitrogen of the basic structure the kinetic constants were calculated for most of them

    Amine functionalization of cholecyst-derived extracellular matrix with generation 1 PAMAM dendrimer

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    This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Biomacromolecules, copyright © American Chemical Society after peer review. To access the final edited and published work, see http://pubs.acs.org/doi/pdf/10.1021/bm701055k.A method to functionalize cholecyst-derived extracellular matrix (CEM) with free amine groups was established in an attempt to improve its potential for tethering of bioactive molecules. CEM was incorporated with Generation-1 polyamidoamine (G1 PAMAM) dendrimer by using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide and N-hydroxysuccinimide cross-linking system. The nature of incorporation of PAMAM dendrimer was evaluated using shrink temperature measurements, Fourier transform infrared (FTIR) assessment, ninhydrin assay, and swellability. The effects of PAMAM incorporation on mechanical and degradation properties of CEM were evaluated using a uniaxial mechanical test and collagenase degradation assay, respectively. Ninhydrin assay and FTIR assessment confirmed the presence of increasing free amine groups with increasing quantity of PAMAM in dendrimer-incorporated CEM (DENCEM) scaffolds. The amount of dendrimer used was found to be critical in controlling scaffold degradation, shrink temperature, and free amine content. Cell culture studies showed that fibroblasts seeded on DENCEM maintained their metabolic activity and ability to proliferate in vitro. In addition, fluorescence cell staining and scanning electron microscopy analysis of cell-seeded DENCEM showed preservation of normal fibroblast morphology and phenotype

    A Glutaraldehyde Free Crosslinking Method for the Treatment of Collagen Based Biomaterials for Clinical Application

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    Biological bioprostheses such as grafts, patches, and heart valves are often derived from biological tissue like the pericardium. These bioprostheses can be of xenogenic, allogeneic, or autologous origin. Irrespective of their origin, all types are pre treated via crosslinking to render the tissue non antigenic and mechanically strong or to minimize degradation. The most widely used crosslinking agent is glutaraldehyde. However, glutaraldehyde treated tissue is prone to calcification, inflammatory degradation, and mechanical injury, and it is incapable of matrix regeneration, leading to structural degeneration over time. In this work, we are investigating an alternative crosslinking method for an intraoperative application. The treated tissue amp;lsquo;s crosslinking degree was evaluated by differential scanning calorimetry. To confirm the findings, a collagenase assay was conducted. Uniaxial tensile testing was used to assess the tissue amp;rsquo;s mechanical properties. To support the findings, the treated tissue was visualized using two photon microscopy. Additionally, fourier transform infrared spectroscopy was performed to study the overall protein secondary structure. Finally, a crosslinking procedure was identified for intraoperative processing. The samples showed a significant increase in thermal and enzymatic stability after treatment compared to the control, with a difference of up to 22.2 amp;deg;C and 100 , respectively. Also, the tissue showed similar biomechanics to glutaraldehyde treated tissue, showing greater extensibility, a higher failure strain, and a lower ultimate tensile strength than the control. The significant difference in the structure band ratio after treatment is proof of the introduction of additional crosslinks compared to the untreated control with regard to differences in the amide I region. The microscopic images support these findings, showing an alteration of the fiber orientation after treatment. For collagen based biomaterials, such as pericardial tissue, the novel phenolic crosslinking agent proved to be an equivalent alternative to glutaraldehyde regarding tissue characteristics. Although long term studies must be performed to investigate superiority in terms of longevity and calcification, our novel crosslinking agent can be applied in concentrations of 1.5 or 2.0 for the treatment of biomaterial

    High-Throughput Identification of Potential Minor Histocompatibility Antigens by MHC Tetramer-Based Screening: Feasibility and Limitations

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    T-cell recognition of minor histocompatibility antigens (MiHA) plays an important role in the graft-versus-tumor (GVT) effect of allogeneic stem cell transplantation (allo-SCT). However, the number of MiHA identified to date remains limited, making clinical application of MiHA reactive T-cell infusion difficult. This study represents the first attempt of genome-wide prediction of MiHA, coupled to the isolation of T-cell populations that react with these antigens. In this unbiased high-throughput MiHA screen, both the possibilities and pitfalls of this approach were investigated. First, 973 polymorphic peptides expressed by hematopoietic stem cells were predicted and screened for HLA-A2 binding. Subsequently a set of 333 high affinity HLA-A2 ligands was identified and post transplantation samples from allo-SCT patients were screened for T-cell reactivity by a combination of pMHC-tetramer-based enrichment and multi-color flow cytometry. Using this approach, 71 peptide-reactive T-cell populations were generated. The isolation of a T-cell line specifically recognizing target cells expressing the MAP4K1IMA antigen demonstrates that identification of MiHA through this approach is in principle feasible. However, with the exception of the known MiHA HMHA1, none of the other T-cell populations that were generated demonstrated recognition of endogenously MiHA expressing target cells, even though recognition of peptide-loaded targets was often apparent
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