Femtosecond spectroscopy of the first events of the photochemical cycle in bacteriorhodopsin

The first steps in the photochemistry of bacteriorhodopsin (BR) are investigated with light pulses of 160 fs duration. Four samples are studied: (i) the purple membrane, (ii) deuterated purple membrane, (iii) BR trimers and (iv) BR monomers. In all samples the first intermediate J is formed within 430±50 fs. No isotope effect is observed in the formation of J upon deuteration, in contrast to previous reports with much higher excitation energies. Thus proton movement to or from the retinal Schiff's base is not relevant during the first step. Comparing the data for trimeric and monomeric BR suggests an upper limit of 50 fs for the transfer of excitation energy from the excitonically coupled trimer to a single retinal chromophore

Stable operation of a synchronously pumped colliding-pulse mode-locked ring dye laser

Pulses of 100-fsec duration are obtained by synchronous pumping of a colliding-pulse ring dye laser with a mode-locked Ar+-ion laser. Stable operation of the synchronously pumped colliding-pulse mode-locked laser over hours was obtained by a suitable choice of the distance between the gain and the absorber in combination with an appro-priate pump-pulse sequence. Passive mode locking of a ring dye laser by the inter-action of two counterpropagating pulses in a thin sat-urable absorber (colliding-pulse mode locking) yields femtosecond laser pulses. ' In these lasers the gain medium (Rhodamine 6G) is pumped by a cw Ar+-ion laser. The saturable absorber (DODCI, 3,3-diethyl-oxadicarbocyanine iodide) synchronizes two counter-propagating pulses meeting in the absorber jet stream. The colliding pulses form a transient grating, which synchronizes and stabilizes the pulses.2 In order to ensure equal amplification for both counterpropagatin

Effectively Solving NP-SPEC Encodings by Translation to ASP

NP-SPEC is a language for specifying problems in NP in a declarative way. Despite the fact that the semantics of the language was given by referring to Datalog with circumscription, which is very close to ASP, so far the only existing implementations are by means of ECLiPSe Prolog and via Boolean satisfiability solvers. In this paper, we present translations from NP-SPEC into ASP, and provide an experimental evaluation of existing implementations and the proposed translations to ASP using various ASP solvers. The results show that translating to ASP clearly has an edge over the existing translation into SAT, which involves an intrinsic grounding process. We also argue that it might be useful to incorporate certain language constructs of NPSPEC into mainstream ASP