A ESTRATÉGIA PRÓ-SAÚDE E FORMAÇÃO EM ENFERMAGEM: CONHECIMENTOS E PRÁTICAS DOS DOCENTES DA FAEN/UERN

O objetivo deste estudo foi apreender conhecimentos e percepções de docentes da Faculdade de Enfermagem (FAEN) da Universidade do Estado do Rio Grande do Norte (UERN) acercado Pró-Saúde enquanto estratégia de articulação ensino/serviço. Para tanto foi desenvolvido um estudo descritivo, de natureza qualitativa, realizado por meio de entrevista com quatro docentes desta Instituição. Dos dados, coletados entre março e abril de 2010, foram construídas três categorias que retratavam o objetivo do estudo, a saber: Conhecimentos acerca do Pró-Saúde; Articulação ensino-serviço e Dificuldades para implementação e efetivação do Pró-Saúde. Dos resultados foi possível apreender as limitações nos conhecimentos e práticas acerca do projeto Pró-Saúde desenvolvidapela FAEN/UERN, o que potencializa as limitações na articulação ensino/serviço. Assim, é preciso refletir o desenvolvimento da estratégia na perspectiva de tornar os diferentes atores capazes de fomentar as mudanças no processo de formação e conformação das práticas em saúde vigentes

Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2

A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs

Soroepidemiologia da doença de Chagas em localidades limítrofes a áreas de risco no Oeste Potiguar

To carry out a seroepidemiological and spatial survey for Chagas Disease in the municipality of Felipe Guerra, located among municipalities with a high prevalence in the Western Mesoregion of the state of Rio Grande do Norte. 423 individuals living in Felipe Guerra / RN were included. Serology for Chagas disease was carried out, questionnaires were applied on risk indicators and knowledge about the disease and home georeferencing. A prevalence of 6.6% (28) of individuals with Chagas disease was identified. There was a predominance of individuals with low schooling, farmers, with low purchasing power and episodes of identification of triatomines in households were reported. The spatial analysis showed the proximity between the homes and the water sources, as well as the municipalities with high prevalence. Given the seroprevalence identified, there is a need to carry out seroepidemiological surveys in municipalities bordering regions characterized by high rates of Chagas disease, since surrounding locations may present similar epidemiological scenarios. Keywords: Chagas Disease. Seroepidemiologic studies. Epidemiology. Health profile.Realizou-se um inquérito soroepidemiológico e espacial para Doença de Chagas no município de Felipe Guerra, situado entre municípios com alta prevalência na Mesorregião Oeste do estado do Rio Grande do Norte. Foram incluídos 423 indivíduos residentes em Felipe Guerra/RN. Realizou-se sorologia para Doença de Chagas, aplicação de questionários sobre indicadores de risco e conhecimento sobre a doença e georreferenciamento domiciliar. Identificou-se uma prevalência de 6,6% (28) de indivíduos acometidos pela condição chagásica. Predominaram indivíduos com baixa escolaridade, agricultores, de reduzido poder aquisitivo e foram referidos episódios de identificação de triatomíneos nos domicílios. A análise espacial mostrou a proximidade entre as residências e as fontes de água, bem como dos municípios de alta prevalência. Dada a soroprevalência identificada, estabelece-se a necessidade para a realização de inquéritos soroepidemiológicos em municípios limítrofes a regiões caracterizadas por elevados índices de Doença de Chagas, uma vez que localidades circunvizinhas podem apresentar cenários epidemiológicos semelhantes. Palavras-chave: Doença de Chagas. Estudos soroepidemiológicos. Epidemiologia. Perfil de saúde

<b>Expression of bradykinin in human placenta from healthy and preeclamptic women

This study evaluated the expression of bradykinin (BK) in human placenta from healthy and preeclamptic women. This is a non-randomized experimental study, in which we performed histological analysis of placental tissue to observe changes that occur in each kind of placenta as well as immunohistochemical analysis to investigate the expression of bradykinin. We used ‘Paleontological Statistics software package for education and data analysis’ 3.06 and R for the statistical analysis. The Ethics Committee of the University of Rio Grande do Norte State approved this experiment under protocol number 166370, according to the determinations established by Resolutions 466/12 and 441/11.We found differences between the two kinds of placenta concerning the diameter of the vessels and the rate of cytotrophoblastic invasion. Student’s t-test evidenced significant difference (p = 7.6395 x 10-5) indicating greater marking of BK per section in the healthy placenta group. The result of more significant expression of bradykinin in healthy placenta can be used as a starting point for deeper researches aiming to better characterize and quantify this expression.

Prevalência da deficiência da glicose-6-fosfato desidrogenase em doadores de sangue de Mossoró, Rio Grande do Norte Prevalence of glucose-6-phosphate dehydrogenase deficiency in blood donors of Mossoró, Rio Grande do Norte

<abstract language="eng">Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzymopathy. It affects as many as 330 million individuals worldwide. This deficiency may determine neonatal jaundice, chronic nonspherocytic hemolytic anemia and acute hemolytic anemia induced by drugs, infections and broad bean ingestion. The efficacy of blood transfusion is decreased when the donor is G6PD deficient. In this study, we aimed at determining the prevalence of G6PD deficiency in blood donors of Mossoro, Brazil. Samples of 714 blood donors (576 men and 138 women; 343 white and 371 non-white) with ages ranging from 18 to 62 years and that accepted to participate in the study were analyzed. All participants answered a standard questionnaire. G6PD activity was analyzed by the methemoglobin reduction test with deficiency being confirmed by the semiquantitative test. The overall prevalence of G6PD deficiency in blood donors was 3.8%, similar to the rate described for others regions of Brazil. There was no significant statistical difference in the frequency of G6PD deficiency between men and women, nor between white and non-white blood donors. This relatively high frequency of G6PD deficiency highlights a need to screen blood donors for this condition