27 research outputs found
A method for validating the accuracy of NMR protein structures
We present a method that measures the accuracy of NMR protein structures. It compares random coil index [RCI] against local rigidity predicted by mathematical rigidity theory, calculated from NMR structures [FIRST], using a correlation score (which assesses secondary structure), and an RMSD score (which measures overall rigidity). We test its performance using: structures refined in explicit solvent, which are much better than unrefined structures; decoy structures generated for 89 NMR structures; and conventional predictors of accuracy such as number of restraints per residue, restraint violations, energy of structure, ensemble RMSD, Ramachandran distribution, and clashscore. Restraint violations and RMSD are poor measures of accuracy. Comparisons of NMR to crystal structures show that secondary structure is equally accurate, but crystal structures are typically too rigid in loops, whereas NMR structures are typically too floppy overall. We show that the method is a useful addition to existing measures of accuracy
Structure-activity studies of peptides from the "hot-spot" region of human CD2 protein: Development of peptides for immunomodulation
10.1021/jm0503547Journal of Medicinal Chemistry48206236-6249JMCM
Un incontro internazionale sugli effetti dell'innalzamento del livello marino
Cyclic constraints are incorporated
into an 11-residue analogue
of the N-terminus of glucagon-like peptide-1 (GLP-1) to investigate
effects of structure on agonist activity. Cyclization through linking
side chains of residues 2 and 5 or 5 and 9 produced agonists at nM
concentrations in a cAMP assay. 2D NMR and CD spectra revealed an
N-terminal β-turn and a C-terminal helix that differentially
influenced affinity and agonist potency. These structures can inform
development of small molecule agonists of the GLP-1 receptor to treat
type 2 diabetes