C-type lectin-like receptors of the dectin-1 cluster : ligands and signaling pathways

Peer reviewedPublisher PD

C-type lectin receptors of the Dectin-1 cluster : Physiological roles and involvement in disease

Acknowledgements: We apologise to our many colleagues whose valuable work we could not cite due to space constraints. Funding was provided by the Wellcome Trust (102705, 097377), the Medical Research Council Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). KT received a research fellowship from Jikei University School of Medicine.Peer reviewedPublisher PD

β-Glucan Grafted Microcapsule, a Tool for Studying the Immunomodulatory Effect of Microbial Cell Wall Polysaccharides

Acknowledgements This study was supported by the Centre Franco-Indien pour la Promotion de la Recherche avancée and Agence nationale de la recherche-Deutsche Forschungsgemeinschaft grants, the Institut Universitaire de France, La région Ile de France, BPI France and benefited from the facilities of MIMA2 MEB-GABI, INRA, Agroparistech, 78352 Jouy-en-Josas, France. Funding sources: CEFIPRA (Centre Franco-Indien pour la Promotion de la Recherche avancée; Grant No. 5403-1, Pathogenic Aspergillus) to JPL and VA, and Agence nationale de la recherche and Deutsche Forschungsgemeinschaft (ANR-DFG bilateral grant, AfuINTERACT) to JPL.Peer reviewedPostprin

Synthesis of the fungal metabolite YWA1 and related constructs as tools to study MelLec-mediated immune response to Aspergillus infections

Funding Sources Funding was provided by the Wellcome Trust (102705, 097377), the Medical Research Council Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1). ACKNOWLEDGMENT We thank Ian Fraser Flow Cytometry and Microscopy and Histology facilities, University of Aberdeen.Peer reviewedPostprin

Signalling through MyD88 drives surface expression of the mycobacterial receptors MCL (Clecsf8, Clec4d) and Mincle (Clec4e) following microbial stimulation

Acknowledgements We would like to thank the staff of the animal facility for their support and care for our animals. Funding was provided by the Wellcome Trust (102705) and Medical Research Council (UK) (MR/J004820/1) and a University of Aberdeen Studentship to BK.Peer reviewedPostprintPublisher PD

Characterization of antifungal C-type lectin receptor expression on murine epithelial and endothelial cells in mucosal tissues

Funding Information: We thank P. Asamaphan, A. Clark, and B. Kerscher for providing NIH overexpression cell lines, S. Yamasaki for the anti‐Mincle antibody, the staff of the University of Aberdeen animal facility for the care for our animals, and the Iain Fraser Cytometry Centre at the University of Aberdeen for their assistance. This work was supported by funding from the Wellcome Trust (102705, 217163), the Medical Research Council Centre for Medical Mycology, and the University of Exeter (MR/N006364/2).Peer reviewedPublisher PD

Aspergillus-induced superoxide production by cystic fibrosis phagocytes is associated with disease severity

We would like to thank the CF patients for their participation and a special thank you goes to CF nurses Karen Griffiths and Sandra Steele (Aberdeen Royal Infirmary, Aberdeen, UK) for their invaluable contribution to this study. Support statement: A. Warris, G.D. Brown, S.F. Brunel and J.A. Willment were supported by the Wellcome Trust Strategic Award (grant 097377) and the MRC Centre for Medical Mycology (grant MR/N006364/1) at the University of Aberdeen. A. Warris and S.F. Brunel were also supported by the Chloe Fund. Funding information for this article has been deposited with the Crossref Funder Registry.Peer reviewedPublisher PD

Dectin-1 Is A Major β-Glucan Receptor On Macrophages

Zymosan is a β-glucan– and mannan-rich particle that is widely used as a cellular activator for examining the numerous responses effected by phagocytes. The macrophage mannose receptor (MR) and complement receptor 3 (CR3) have historically been considered the major macrophage lectins involved in the nonopsonic recognition of these yeast-derived particles. Using specific carbohydrate inhibitors, we show that a β-glucan receptor, but not the MR, is a predominant receptor involved in this process. Furthermore, nonopsonic zymosan binding was unaffected by genetic CD11b deficiency or a blocking monoclonal antibody (mAb) against CR3, demonstrating that CR3 was not the β-glucan receptor mediating this activity. To address the role of the recently described β-glucan receptor, Dectin-1, we generated a novel anti–Dectin-1 mAb, 2A11. Using this mAb, we show here that Dectin-1 was almost exclusively responsible for the β-glucan–dependent, nonopsonic recognition of zymosan by primary macro-phages. These findings define Dectin-1 as the leukocyte β-glucan receptor, first described over 50 years ago, and resolves the long-standing controversy regarding the identity of this important molecule. Furthermore, these results identify Dectin-1 as a new target for examining the immunomodulatory properties of β-glucans for therapeutic drug design

MelLec Exacerbates the Pathogenesis of Aspergillus fumigatus-Induced Allergic Inflammation in Mice

ACKNOWLEDGMENTS We thank the staff of the University of Aberdeen animal facility for their support and care for our animals. We also would like to express gratitude to Linda Duncan and Ailsa Laird of the Ian Fraser Cytometry Centre at the University of Aberdeen for their technical support and advice. Funding was provided by the Wellcome Trust (102705, 097377) and the Medical Research Council Centre for Medical Mycology (MR/N006364/2). For the purpose of open access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission. KT received a research fellowship from The Jikei University School of Medicine.Peer reviewedPublisher PD

Mannan detecting C-type lectin receptor probes recognise immune epitopes with diverse chemical, spatial and phylogenetic heterogeneity in fungal cell walls

Funding Information: This work was supported by the Wellcome Trust Investigator, Collaborative, Equipment, Strategic and Biomedical Resource awards (086827, 075470, 097377, 101873, 200208, 093378 and 099197), the Applied Molecular Biosciences Unit-UCIBIO (FCT/MCTES UID/Multi/04378/2019), Wellcome Trust Biomedical Resource grant (108430/Z/15/Z), March of Dimes (Arlington, Virginia, U.S.A.) Prematurity Research Center grant (22-FY18-821) and by the MRC Centre for Medical Mycology (N006364/1). The University of Aberdeen funded a studentship to IV as part of NARG?s Wellcome Senior Investigator Award. https://wellcome.ac.uk/ - Wellcome. https://mrc.ukri.org/ - MRC. https:// www.requimte.pt/ucibio/ - the Applied Molecular Biosciences Unit-UCIBIO. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2020 Vendele et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Peer reviewedPublisher PD