Endothelial and smooth muscle cell interactions with a PCL-PU composite vascular scaffold with potential for bioactive release

Paper discussing endothelial and smooth muscle cell interactions with a PCL-PU composite vascular scaffold with potential for bioactive release

Novel biodegradable fibres for applications in tissue engineering and drug delivery

The preparation and characterisation of novel biodegradable polymer fibres for application in tissue engineering and drug delivery are reported. Poly(e-caprolactone) (PCL) fibres were produced by wet spinning from solutions in acetone under low shear (gravity flow) conditions. The tensile strength and stiffness of as-spun fibres were highly dependent on the concentration of the spinning solution. Use of a 6% w/v solution resulted in fibres having strength and stiffness of 1.8 MPa and 0.01 GPa respectively, whereas these values increased to 9.9 MPa and 0.1 GPa when fibres were produced from 20% w/v solutions. Cold drawing to an extension of 500% resulted in further increases in fibre strength (up to 50 MPa) and stiffness (0.3 GPa). Hot drawing to 500% further increased the fibre strength (up to 81 MPa) and stiffness (0.5 GPa). The surface morphology of as-spun fibres was modified, to yield a directional grooved pattern by drying in contact with a mandrel having a machined topography characterised by a peak-peak separation of 91 mm and a peak height of 30 mm. Differential scanning calorimetery (DSC) analysis of as-spun fibres revealed the characteristic melting point of PCL at around 58°C and a % crystallinity of approximately 60%. The biocompatibility of as-spun fibres was assessed using cell culture. The number of attached 3T3 Swiss mouse fibroblasts, C2C12 mouse myoblasts and human umbilical vein endothelial cells (HUVECs) on as-spun, 500% cold drawn, and gelatin coated PCL fibres were observed. The results showed that the fibres promoted cell proliferation for 9 days in cell culture and was slightly lower than on tissue culture plastic. The morphology of all cell lines was assessed on the various PCL fibres using scanning electron microscopy. The cell function of HUVECs growing on the as-spun PCL fibres was evaluated. The ability HUVECs to induce an immune response when stimulated with lipopolysaccaride (LPS) and thereby to increase the amount of cell surface receptors was assessed by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR). The results showed that PCL fibres did not inhibit this function compared to TCP. As-spun PCL fibres were loaded with 1 % ovine albumin (OVA) powder, 1% OVA nanoparticles and 5% OVA nanoparticles by weight and the protein release was assessed in vitro. PCL fibres loaded with 1 % OVA powder released 70%, 1% OVA nanoparticle released 60% and the 5% OVA nanoparticle released 25% of their protein content over 28 days. These release figures did not alter when the fibres were subjected to lipase enzymatic degradation. The OVA released was examined for structural integrity by SDS-PAGE. This showed that the protein molecular weight was not altered after incorporation into the fibres. The bioactivity of progesterone was assessed following incorporation into PCL fibres. Results showed that the progesterone released had a pronounced effect on MCF-7 breast epithelial cells, inhibiting their proliferation. The PCL fibres display high fibre compliance, a potential for controlling the fibre surface architecture to promote contact guidance effects, favorable proliferation rate of fibroblasts, myoblasts and HUVECs and the ability to release pharmaceuticals. These properties recommended their use for 3-D scaffold production in soft tissue engineering and the fibres could also be exploited for controlled presentation and release of biopharmaceuticals such as growth factors

The Phases Differential Astrometry Data Archive. IV. The Triple Star Systems 63 Gem A and HR 2896

Differential astrometry measurements from the Palomar High-precision Astrometric Search for Exoplanet Systems (PHASES) are used to constrain the astrometric orbit of the previously known ≾2 day subsystem in the triple system 63 Gem A and have detected a previously unknown two-year Keplerian wobble superimposed on the visual orbit of the much longer period (213 years) binary system HR 2896. 63 Gem A was already known to be triple from spectroscopic work, and absorption lines from all three stars can be identified and their individual Doppler shifts measured; new velocities for all three components are presented to aid in constraining the orbit and measuring the stellar masses. In fact, 63 Gem itself is a sextuple system: the hierarchical triple (Aa1-Aa2)-Ab (in which Aa1 and Aa2 orbit each other with a rapid period just under 2 days, and Ab orbits these every two years), plus three distant common proper motion companions. The very small astrometric perturbation caused by the inner pair in 63 Gem A stretches the limits of current astrometric capabilities, but PHASES observations are able to constrain the orientation of the orbit. The two bright stars comprising the HR 2896 long-period (213 year) system have a combined spectral type of K0III and the newly detected object's mass estimate places it in the regime of being an M dwarf. The motion of the stars are slow enough that their spectral features are always blended, preventing Doppler studies. The PHASES measurements and radial velocities (when available) have been combined with lower precision single-aperture measurements covering a much longer time frame (from eyepiece measurements, speckle interferometry, and adaptive optics) to improve the characterization of the long-period orbits in both binaries. The visual orbits of the short- and long-period systems are presented for both systems and used to calculate two possible values of the mutual inclinations between inner and outer orbits of 152° ± 12° or a less likely value of 31° ± 11° for 63 Gem A and 10.°2 ± 2.°4 or 171.°2 ± 2.°8 for HR 2896. The first is not coplanar, whereas the second is either nearly coplanar or anti-coplanar

Trichophycins B–F, Chlorovinylidene-Containing Polyketides Isolated from a Cyanobacterial Bloom

NMR-guided isolation (based on 1D 1H and 13C NMR resonances consistent with a chlorovinylidene moiety) resulted in the characterization of five new highly functionalized polyketides, trichophycins B-F (1-5) and one non-chlorinated metabolite tricholactone (6) from a collection of Trichodesmium bloom material from the Gulf of Mexico. The planar structures of 1-6 were determined using 1D and 2D NMR spectroscopy, mass spectrometry and complementary spectroscopic procedures. Absolute configuration analysis of 1 and 2 were carried out by 1H NMR analysis of diastereomeric Mosher esters in addition to ECD spectroscopy, J-based configuration analysis and DFT calculations. The absolute configurations of 3-6 were proposed based on comparative analysis of 13C NMR chemical shifts, relative configurations, and optical rotation values to compounds 1 and 2. Compounds 1-5 represent new additions to the trichophycin family and are hallmarked by a chlorovinylidene moiety. These new trichophycins and tricholactone (1-6) feature intriguing variations with respect to putative biosynthetic starting units, halogenation, and terminations and trichophycin E (4) features a rare alkynyl bromide functionality. The phenyl-containing trichophycins showed low cytotoxicity to neuro-2A cells, while the alkyne-containing trichophycins showed no toxicity

Drug-resistant Escherichia coli, Rural Idaho

Stool carriage of drug-resistant Escherichia coli in home-living residents of a rural community was examined. Carriage of nalidixic acid–resistant E. coli was associated with recent use of antimicrobial agents in the household. Household clustering of drug-resistant E. coli was observed. Most carriers of drug-resistant E. coli lacked conventional risk factors

Scaling Properties of Dimensionality Reduction for Neural Populations and Network Models

Recent studies have applied dimensionality reduction methods to understand how the multi-dimensional structure of neural population activity gives rise to brain function. It is unclear, however, how the results obtained from dimensionality reduction generalize to recordings with larger numbers of neurons and trials or how these results relate to the underlying network structure. We address these questions by applying factor analysis to recordings in the visual cortex of non-human primates and to spiking network models that self-generate irregular activity through a balance of excitation and inhibition. We compared the scaling trends of two key outputs of dimensionality reduction—shared dimensionality and percent shared variance—with neuron and trial count. We found that the scaling properties of networks with non-clustered and clustered connectivity differed, and that the in vivo recordings were more consistent with the clustered network. Furthermore, recordings from tens of neurons were sufficient to identify the dominant modes of shared variability that generalize to larger portions of the network. These findings can help guide the interpretation of dimensionality reduction outputs in regimes of limited neuron and trial sampling and help relate these outputs to the underlying network structure

Orbital Solutions and Absolute Elements of the Short-Period Eclipsing Binary ES Librae

We have obtained new differential UBV photoelectric photometry and radial velocities of both components of the short-period eclipsing binary ES Lib. The system has a circular orbit with a period of 0.883040928 days and is seen at an inclination of 70.1°. With the Wilson-Devinney analysis program, we obtained a simultaneous solution of our photometric and spectroscopic observations that resulted in masses of M1 = 2.30 ± 0.03 M⊙ and M2 = 0.97 ± 0.01 M⊙ and the equal-volume radii of R1 = 2.69 ± 0.02 R⊙ and R2 = 1.83 ± 0.01 R⊙ for the primary and secondary, respectively. The secondary is oversized and overluminous for its mass. The effective temperatures of the primary and secondary are 8500 K (fixed) and 5774 ± 57 K, respectively. Despite the very large temperature difference, our photometric and spectroscopic data indicate that ES Lib is not semidetached but rather require it to be in an overcontact state, where both components exceed their critical Roche lobes. Given its nonthermal equilibrium state, if the overcontact solution correctly characterizes the system, the change from being semidetached to overcontact may have occurred recently. While the asymmetry of the light curves can be modeled well with a large, hot starspot or a large, cool one on the secondary component, we prefer the latter interpretation because cool spots are a typical feature on many contact binaries

The orbits of the quadruple star system 88 Tau A from PHASES differential astrometry and radial velocity

We have used high precision differential astrometry from the Palomar High-precision Astrometric Search for Exoplanet Systems (PHASES) project and radial velocity measurements covering a time-span of 20 years to determine the orbital parameters of the 88 Tau A system. 88 Tau is a complex hierarchical multiple system comprising a total of six stars; we have studied the brightest 4, consisting of two short-period pairs orbiting each other with an 18-year period. We present the first orbital solution for one of the short-period pairs, and determine the masses of the components and distance to the system to the level of a few percent. In addition, our astrometric measurements allow us to make the first determination of the mutual inclinations of the orbits. We find that the sub-systems are not coplanar.Comment: Corrected Author Ordering; 12 Pages, Accepted for publication in Ap

Population activity structure of excitatory and inhibitory neurons

Many studies use population analysis approaches, such as dimensionality reduction, to characterize the activity of large groups of neurons. To date, these methods have treated each neuron equally, without taking into account whether neurons are excitatory or inhibitory. We studied population activity structure as a function of neuron type by applying factor analysis to spontaneous activity from spiking networks with balanced excitation and inhibition. Throughout the study, we characterized population activity structure by measuring its dimensionality and the percentage of overall activity variance that is shared among neurons. First, by sampling only excitatory or only inhibitory neurons, we found that the activity structures of these two populations in balanced networks are measurably different. We also found that the population activity structure is dependent on the ratio of excitatory to inhibitory neurons sampled. Finally we classified neurons from extracellular recordings in the primary visual cortex of anesthetized macaques as putative excitatory or inhibitory using waveform classification, and found similarities with the neuron type-specific population activity structure of a balanced network with excitatory clustering. These results imply that knowledge of neuron type is important, and allows for stronger statistical tests, when interpreting population activity structure