Administration of either anti-CD40 or interleukin-12 following lethal total body irradiation induces acute lethal toxicity affecting the gut

AbstractInterleukin (IL)-12 and antibodies against CD40 have demonstrated antitumor effects in a variety of in vivo model systems. However, both agents can also mediate significant toxicities either when used following lethal TBI or when administered in combination with other agents such as IL-2. In this study, we assessed the effects of anti-CD40 monoclonal antibody (MoAb) and IL-12 in lethally irradiated mice. Acute lethal toxicity was observed following the administration of either 10 microg anti-CD40 MoAb (FGK45) or 0.5 microg of recombinant murine (rm)IL-12 that resulted in 100% mortality of all mice within 4 to 6 days. Histological evaluation revealed destruction of the normal gut architecture in both anti-CD40 MoAb- and rmIL-12-treated mice. Analysis of serum cytokine levels in the lethally irradiated mice receiving anti-CD40 MoAb demonstrated a marked increase of interferon (IFN)-gamma and IL-12 p40, whereas mice receiving rmIL-12 demonstrated a marked increase of IFN-gamma. Lethally irradiated IL-12 p40 knock-out mice were resistant to anti-CD40-induced toxicity, suggesting that the lack of IL-12 p40 with no possibility of making functional IL- 12 p70 is key for this toxic reaction. Similarly, lethally irradiated IFN-gamma knock-out mice were completely resistant to rmIL-12-induced toxicity, suggesting that IFN-gamma is a major player in IL-12-mediated toxicity. These results suggest that both anti-CD40 MoAb and rmIL-12 induce an acute fatal toxicity characterized by similar intestinal pathology and mediated in part by IFN-gamma.Biol Blood Marrow Transplant 2002;8(6):316-25

Submersible observations of deep-reef fishes of Heceta Bank, Oregon

Rockfishes, Sebastes spp.. were the most numerous and speciose fishes seen during 16 submersible dives from 64 to 305 m depth in the vicinity of Heceta Bank off the coast of Oregon. Dense schools of juvenile rockfishes and large yellowtail rockfish, S. flavidus, were observed only over rocky, high relief areas near the top of the bank, and highest densities of small benthic rockfishes (up to 5-10/m2) on the flanks of the bank. These observations suggest that shallow, rocky portions of Heceta Bank are a nursery area for juvenile rockfishes. Two species groups of nonschooling fishes were identified based on transects over the diverse seafloor habitats around the bank: one comprised primarily of rockfishes in shallow water on rock and cobble, and the other comprised of flatfishes, agonids, sablefish, and some rockfishes in deep water over mud and cobble. Species composition of fishes observed from submersible dives differed from species composition of fishes taken from trawl catches in the same general areas.This publication is associated with the document Fish Assemblages of Rocky Banks of the Pacific Northwest: Final Report by M.A. Hixon, B.N. Tissot, and W.G. Pearcy, and its accompanying data reports

SURVIVAL, GROWTH, AND BEHAVIOR OF THE LOLIGINID SQUIDS LOLIGO PLEI, LOLIGO PEALEI, AND LOLLIGUNCULA BREVIS (MOLLUSCA: CEPHALOPODA) IN CLOSED SEA WATER SYSTEMS

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Fish-habitat associations on a deep reef at the edge of the Oregon continental shelf

Heceta Bank is a large reef on the edge of the central Oregon continental shelf that supports a wide variety of commercial fisheries. Using the research submersible Delta, we studied fish abundances on Heceta Bank and the relationship between species composition of fish assemblages and bottom types. Cluster analysis indicated that fish assemblages were most unique on mud, boulder, rock ridge, mud and cobble, and mud and boulder substrates. Rockfishes, particularly pygmy Sebastes wilsoni, sharpchin S. zacentrus, rosethorn S. helvomaculatus, and yellowtail S. flavidus, were the most abundant fishes and dominated all substrates except mud, where Dover sole Microstomus pacificus and zoarcids Lycodes pacificus were most abundant. Principal component analysis (PCA) and canonical correlation analysis (CCA) were used to determine the sources of variation within the data. PCA demonstrated that habitat variability was a fundamental cause of heterogeneity among fish assemblages. In contrast, CCA showed how species occurrences were related to specific substrates. Ontogenetic shifts in behavior and substrate preference occurred in pygmy rockfish. Small juveniles often formed dense schools above the bank's shallower rocky ridges. Larger individuals occurred in non-polarized assemblages on the bottom in cobble and boulder fields