2,899 research outputs found
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid generation of such probes using the polymerase chain reaction (PCR), and demonstrate their chromosome specificity by fluorescence in situ hybridization to normal human metaphase spreads and interphase nuclei. Oligonucleotide primers for conserved regions of the alpha satellite monomer were used to generate chromosome-specific DNA probes from somatic hybrid cells containing various human chromosomes, and from DNA libraries from sorted human chromosomes. Oligonucleotide primers for chromosome-specific regions of the alpha satellite monomer were used to generate specific DNA probes for the pericentromeric heterochromatin of human chromosomes 1, 6, 7, 17 and X directly from human genomic DNA
Proteome Dynamics with Heavy Water — Instrumentations, Data Analysis, and Biological Applications
The quantitative assessment of the synthesis of individual proteins has been greatly hindered by the lack of a high-throughput nonradioactive method. We recently developed a method that we call “proteome dynamics” and software that enables high-throughput kinetic analyses of peptides on a proteome-wide scale. Previous studies established that oral administration of heavy water (2H2O or deuterium oxide, D2O) is safe and well tolerated in humans. Briefly, a loading dose of 2H2O, a nonradioactive isotope, is administered in drinking water. 2H2O rapidly labels body water and transfers 2H from 2H2O to 2H-labeled amino acids, which incorporates into proteins dependent upon the rate of synthesis of the specific protein. Proteins are analyzed by high-resolution mass spectrometry and protein synthesis is calculated using specialized software. We have established the effectiveness of this method for plasma and mitochondrial proteins. We demonstrated that fasting has a differential effect on the synthesis rates of proteins. We also applied this method to assess the effect of heart failure on the stability of mitochondrial proteins. In this review, we describe the study design, instrumentation, data analysis, and biological application of heavy water-based proteome turnover studies. We summarize this chapter with the challenges in the field and future directions
Bostonia: The Boston University Alumni Magazine. Volume 33
Founded in 1900, Bostonia magazine is Boston University's main alumni publication, which covers alumni and student life, as well as university activities, events, and programs
An Evaluation of Hand-Held Infrared Thermography for Determining Velvet Antler Growth Rates in Red Deer Stags
Last updated: 6/12/200
Observational Versus Electronic Methods for the Detection of Estrus in Farmed Red Deer Hinds
Last updated: 6/12/200
Fluctuations of water near extended hydrophobic and hydrophilic surfaces
We use molecular dynamics simulations of the SPC-E model of liquid water to
derive probability distributions for water density fluctuations in probe
volumes of different shapes and sizes, both in the bulk as well as near
hydrophobic and hydrophilic surfaces. To obtain our results, we introduce a
biased sampling of coarse-grained densities, which in turn biases the actual
solvent density. The technique is easily combined with molecular dynamics
integration algorithms. Our principal result is that the probability for
density fluctuations of water near a hydrophobic surface, with or without
surface-water attractions, is akin to density fluctuations at the water-vapor
interface. Specifically, the probability of density depletion near the surface
is significantly larger than that in bulk. In contrast, we find that the
statistics of water density fluctuations near a model hydrophilic surface are
similar to that in the bulk
Composite repetition-aware data structures
In highly repetitive strings, like collections of genomes from the same
species, distinct measures of repetition all grow sublinearly in the length of
the text, and indexes targeted to such strings typically depend only on one of
these measures. We describe two data structures whose size depends on multiple
measures of repetition at once, and that provide competitive tradeoffs between
the time for counting and reporting all the exact occurrences of a pattern, and
the space taken by the structure. The key component of our constructions is the
run-length encoded BWT (RLBWT), which takes space proportional to the number of
BWT runs: rather than augmenting RLBWT with suffix array samples, we combine it
with data structures from LZ77 indexes, which take space proportional to the
number of LZ77 factors, and with the compact directed acyclic word graph
(CDAWG), which takes space proportional to the number of extensions of maximal
repeats. The combination of CDAWG and RLBWT enables also a new representation
of the suffix tree, whose size depends again on the number of extensions of
maximal repeats, and that is powerful enough to support matching statistics and
constant-space traversal.Comment: (the name of the third co-author was inadvertently omitted from
previous version
Nurses\u27 Alumnae Association Bulletin - Volume 5 Number 8
Calling All Nurses
Financial Report
Calendar of Events
Lest You Forget!
Attention
Review of the Alumnae Association Meetings
President\u27s Report
Barton Memorial Division
Oxygen Therapy
Welcome, White Haven Alumnae
Clinical Use of Penicillin in Infections of the Ears, Nose and Throat
Address - Graduation of Nurses, 1945
Miscellaneous Items
The Blood that Kills
The Story of Malaria
Program
Prizes - May, 1946
Capping Exercises
The Economic Security Program of the Pennsylvania State Nurses\u27 Association
The Clara Melville Scholarship Fund
Card of Thanks
The Poet\u27s Corner
The Hospital Pharmacy
Jefferson Medical College Hospital School of Nursing Faculty
Jefferson Hospital Gray Lady Unite, A.R.R.
The Volunteer Nurses\u27 Aides Salute Jefferson Nurses
Changes in the Staff at Jefferson Hospital
Red Cross Recruits
Did You Know That
The Pennsylvania Nurse
Medical College News
Magazine and Newspaper Items
Central Dressing Room and Transfusion Unit
Rules Concerning Central Dressing Room
Radios and Electrical Appliances
Attending College
Nurses in Anesthesia
Condolences
Marriages
New Arrivals
Deaths
The Bulletin Committee
Attention, Alumnae
New Addresse
Uncoupling of Genomic and Epigenetic Signals in the Maintenance and Inheritance of Heterochromatin Domains in Fission Yeast
Many essential aspects of genome function, including gene expression and chromosome segregation, are mediated throughout development and differentiation by changes in the chromatin state. Along with genomic signals encoded in the DNA, epigenetic processes regulate heritable gene expression patterns. Genomic signals such as enhancers, silencers, and repetitive DNA, while required for the establishment of alternative chromatin states, have an unclear role in epigenetic processes that underlie the persistence of chromatin states throughout development. Here, we demonstrate in fission yeast that the maintenance and inheritance of ectopic heterochromatin domains are independent of the genomic sequences necessary for their de novo establishment. We find that both structural heterochromatin and gene silencing can be stably maintained over an ∼10-kb domain for up to hundreds of cell divisions in the absence of genomic sequences required for heterochromatin establishment, demonstrating the long-term persistence and stability of this chromatin state. The de novo heterochromatin, despite the absence of nucleation sequences, is also stably inherited through meiosis. Together, these studies provide evidence for chromatin-dependent, epigenetic control of gene silencing that is heritable, stable, and self-sustaining, even in the absence of the originating genomic signals
Explanation of the Gibbs paradox within the framework of quantum thermodynamics
The issue of the Gibbs paradox is that when considering mixing of two gases
within classical thermodynamics, the entropy of mixing appears to be a
discontinuous function of the difference between the gases: it is finite for
whatever small difference, but vanishes for identical gases. The resolution
offered in the literature, with help of quantum mixing entropy, was later shown
to be unsatisfactory precisely where it sought to resolve the paradox.
Macroscopic thermodynamics, classical or quantum, is unsuitable for explaining
the paradox, since it does not deal explicitly with the difference between the
gases. The proper approach employs quantum thermodynamics, which deals with
finite quantum systems coupled to a large bath and a macroscopic work source.
Within quantum thermodynamics, entropy generally looses its dominant place and
the target of the paradox is naturally shifted to the decrease of the maximally
available work before and after mixing (mixing ergotropy). In contrast to
entropy this is an unambiguous quantity. For almost identical gases the mixing
ergotropy continuously goes to zero, thus resolving the paradox. In this
approach the concept of ``difference between the gases'' gets a clear
operational meaning related to the possibilities of controlling the involved
quantum states. Difficulties which prevent resolutions of the paradox in its
entropic formulation do not arise here. The mixing ergotropy has several
counter-intuitive features. It can increase when less precise operations are
allowed. In the quantum situation (in contrast to the classical one) the mixing
ergotropy can also increase when decreasing the degree of mixing between the
gases, or when decreasing their distinguishability. These points go against a
direct association of physical irreversibility with lack of information.Comment: Published version. New title. 17 pages Revte
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