Three new triterpene saponins from roots of <i>Eryngium planum</i>

<div><p>Saponin composition of the roots of <i>Eryngium planum</i> L. was investigated. Triterpene saponins found in <i>E. planum</i> and also present in <i>Eryngium maritimum</i> were different from those described previously in <i>Eryngium campestre</i> L. Three primary saponins were isolated and their tentative identifications, based on the electrospray MS/MS fragmentation patterns, were subsequently confirmed by 1D and 2D NMR analyses. Their structures were established as 3-<i>O</i>-β-d-glucopyranosyl-(1 → 2)-β-d-glucuronopyranosyl-21-<i>O</i>-acetyl-22-<i>O</i>-angeloyl-R1-barrigenol (<b>1</b>) and 3-<i>O</i>-β-d-glucopyranosyl-(1 → 2)-β-d-glucuronopyranosyl-22-<i>O</i>-angeloyl-A1-barrigenol (<b>2</b>) and 3-<i>O</i>-β-d-glucopyranosyl-(1 → 2)-β-d-glucuronopyranosyl-22-<i>O</i>-angeloyl-R1-barrigenol (<b>3</b>). Concentrations of the newly identified compounds in aerial parts and roots of both species were estimated using the liquid chromatography–mass spectrometry method.</p></div

Multidirectional characterisation of chemical composition and health-promoting potential of <i>Rosa rugosa</i> hips

<p>Rugosa rose provides one of the largest hips frequently used in the preparation of pharmaceutical and food products. The aim of work was to conduct multidirectional study of biological activity and chemical composition of <i>Rosa rugosa</i> hips. Antiradical, cytotoxic (against cervical and breast cancer cell lines), antibacterial (against eight bacterial strains) and antifungal potential of the species in question was evaluated. Total contents of phenolics, phenolic acids, flavonoids, tannins, carotenoids and ascorbic acid were determined. LC–ESI–MS/MS analysis was performed in order to investigate closely phenolic acids and flavonoid glycosides. As a result, interesting selective cytotoxic effects on cervical (HeLa) and breast cancer (T47D) cell lines, significant antiradical activity (EC₅₀ 2.45 mg mg⁻¹ DPPH^•) and moderate antimicrobial potential (MIC 0.625–1.25 mg mL⁻¹) were observed. Nine phenolic acids and 11 flavonoid glycosides were qualitatively and quantitatively determined, including 7 compounds previously not reported in <i>R. rugosa</i> hips.</p

Triterpene Saponins from the Aerial Parts of Trifolium medium L. var. <i>sarosiense</i>

Seven previously unreported triterpene glycosides (<b>1</b>–<b>7</b>) were isolated from methanol extract of the aerial parts of Trifolium medium var. <i>sarosiense</i> (zigzag clover). Their structures were established by the extensive use of 1D and 2D NMR experiments along with ESI-MS and HRMS analyses. Compounds <b>1</b>–<b>7</b> are oleanane derivatives characterized by the presence of a keto group at C-22 of an aglycone and a primary alcoholic function at C-24 and differing functions at C-30. Among these, compounds <b>1</b>–<b>3</b> and <b>6</b> showed a secondary alcoholic function at C-11, which is methoxylated in compounds <b>4</b> and <b>7</b>. Compound <b>5</b> was shown to possess a known aglycone, wistariasapogenol A; however, it is described here for the first time as a saponin constituent of the <i>Trifolium</i> genus. Some aspects of taxonomic classification of zigzag clover are also discussed