3,595 research outputs found
Architecture of coatomer: Molecular characterization of delta-COP and protein interactions within the complex
Copyright © 2011 by The Rockefeller University Press.Coatomer is a cytosolic protein complex that forms the coat of COP I-coated transport vesicles. In our attempt to analyze the physical and functional interactions between its seven subunits (coat proteins, [COPs] alpha-zeta), we engaged in a program to clone and characterize the individual coatomer subunits. We have now cloned, sequenced, and overexpressed bovine alpha-COP, the 135-kD subunit of coatomer as well as delta-COP, the 57-kD subunit and have identified a yeast homolog of delta-COP by cDNA sequence comparison and by NH2-terminal peptide sequencing. delta-COP shows homologies to subunits of the clathrin adaptor complexes AP1 and AP2. We show that in Golgi-enriched membrane fractions, the protein is predominantly found in COP I-coated transport vesicles and in the budding regions of the Golgi membranes. A knock-out of the delta-COP gene in yeast is lethal. Immunoprecipitation, as well as analysis exploiting the two-hybrid system in a complete COP screen, showed physical interactions between alpha- and epsilon-COPs and between beta- and delta-COPs. Moreover, the two-hybrid system indicates interactions between gamma- and zeta-COPs as well as between alpha- and beta' COPs. We propose that these interactions reflect in vivo associations of those subunits and thus play a functional role in the assembly of coatomer and/or serve to maintain the molecular architecture of the complex.This work was supported by The Deutsche Forschungsgemeinschaft (SFB 352), the Human Frontier Science Program, and the Swiss National Science Foundation No. 31-43366.95
TCF1(+) hepatitis C virus-specific CD8(+) T cells are maintained after cessation of chronic antigen stimulation.
Differentiation and fate of virus-specific CD8(+) T cells after cessation of chronic antigen stimulation is unclear. Here we show that a TCF1(+)CD127(+)PD1(+) hepatitis C virus (HCV)-specific CD8(+) T-cell subset exists in chronically infected patients with phenotypic features of T-cell exhaustion and memory, both before and after treatment with direct acting antiviral (DAA) agents. This subset is maintained during, and for a long duration after, HCV elimination. After antigen re-challenge the less differentiated TCF1(+)CD127(+)PD1(+) population expands, which is accompanied by emergence of terminally exhausted TCF1-CD127-PD1(hi) HCV-specific CD8(+) T cells. These results suggest the TCF1(+)CD127(+)PD1(+) HCV-specific CD8(+) T-cell subset has memory-like characteristics, including antigen-independent survival and recall proliferation. We thus provide evidence for the establishment of memory-like virus-specific CD8(+) T cells in a clinically relevant setting of chronic viral infection and we uncover their fate after cessation of chronic antigen stimulation, implicating a potential strategy for antiviral immunotherapy
Towards Loop Quantization of Plane Gravitational Waves
The polarized Gowdy model in terms of Ashtekar-Barbero variables is further
reduced by including the Killing equations for plane-fronted parallel
gravitational waves with parallel rays. The resulting constraint algebra,
including one constraint derived from the Killing equations in addition to the
standard ones of General Relativity, are shown to form a set of first-class
constraints. Using earlier work by Banerjee and Date the constraints are
expressed in terms of classical quantities that have an operator equivalent in
Loop Quantum Gravity, making space-times with pp-waves accessible to loop
quantization techniques.Comment: 14 page
Beyond Einstein-Cartan gravity: Quadratic torsion and curvature invariants with even and odd parity including all boundary terms
Recently, gravitational gauge theories with torsion have been discussed by an
increasing number of authors from a classical as well as from a quantum field
theoretical point of view. The Einstein-Cartan(-Sciama-Kibble) Lagrangian has
been enriched by the parity odd pseudoscalar curvature (Hojman, Mukku, and
Sayed) and by torsion square and curvature square pieces, likewise of even and
odd parity. (i) We show that the inverse of the so-called Barbero-Immirzi
parameter multiplying the pseudoscalar curvature, because of the topological
Nieh-Yan form, can only be appropriately discussed if torsion square pieces are
included. (ii) The quadratic gauge Lagrangian with both parities, proposed by
Obukhov et al. and Baekler et al., emerges also in the framework of Diakonov et
al.(2011). We establish the exact relations between both approaches by applying
the topological Euler and Pontryagin forms in a Riemann-Cartan space expressed
for the first time in terms of irreducible pieces of the curvature tensor.
(iii) Only in a Riemann-Cartan spacetime, that is, in a spacetime with torsion,
parity violating terms can be brought into the gravitational Lagrangian in a
straightforward and natural way. Accordingly, Riemann-Cartan spacetime is a
natural habitat for chiral fermionic matter fields.Comment: 12 page latex, as version 2 an old file was submitted by mistake,
this is now the real corrected fil
A new study of Mg(,n)Si angular distributions at = 3 - 5 MeV
The observation of Al gives us the proof of active nucleosynthesis in
the Milky Way. However the identification of the main producers of Al is
still a matter of debate. Many sites have been proposed, but our poor knowledge
of the nuclear processes involved introduces high uncertainties. In particular,
the limited accuracy on the Mg(,n)Si reaction cross
section has been identified as the main source of nuclear uncertainty in the
production of Al in C/Ne explosive burning in massive stars, which has
been suggested to be the main source of Al in the Galaxy. We studied
this reaction through neutron spectroscopy at the CN Van de Graaff accelerator
of the Legnaro National Laboratories. Thanks to this technique we are able to
discriminate the (,n) events from possible contamination arising from
parasitic reactions. In particular, we measured the neutron angular
distributions at 5 different beam energies (between 3 and 5 MeV) in the
\ang{17.5}-\ang{106} laboratory system angular range. The presented results
disagree with the assumptions introduced in the analysis of a previous
experiment.Comment: 9 pages, 9 figures - accepted by EPJ
Assessing Pulmonary Perfusion in Emphysema Automated Quantification of Perfused Blood Volume in Dual-Energy CTPA
Objectives: The objective of this study was to determine whether automated quantification of lung perfused blood volume (PBV) in dual-energy computed tomographic pulmonary angiography (DE-CTPA) can be used to assess the severity and regional distribution of pulmonary hypoperfusion in emphysema. Materials and Methods: We retrospectively analyzed 40 consecutive patients (mean age, 67 13] years) with pulmonary emphysema, who have no cardiopulmonary comorbidities, and a DE-CTPA negative for pulmonary embolism. Automated quantification of global and regional pulmonary PBV was performed using the syngo Dual Energy application (Siemens Healthcare). Similarly, the global and regional degrees of parenchymal hypodensity were assessed automatically as the percentage of voxels with a computed tomographic density less than -900 Hounsfield unit. Emphysema severity was rated visually, and pulmonary function tests were obtained by chart review, if available. Results: Global PBV generated by automated quantification of pulmonary PBV in the DE-CTPA data sets showed a moderately strong but highly significant negative correlation with residual volume in percentage of the predicted residual volume (r = -0.62; P = 0.002; n = 23) and a positive correlation with forced expiratory volume in 1 second in percentage of the predicted forced expiratory volume in 1 second (r = 0.67; P < 0.001; n = 23). Global PBV values strongly correlated with diffusing lung capacity for carbon monoxide (r = 0.80; P < 0.001; n = 15). Pulmonary PBV values decreased with visual emphysema severity (r = -0.46, P = 0.003, n = 40). Moderate negative correlations were found between global PBV values and parenchymal hypodensity both in a per-patient (r = -0.63; P G 0.001; n = 40) and per-region analyses (r = -0.62; P < 0.001; n = 40). Conclusions: Dual-energy computed tomographic pulmonary angiography allows simultaneous assessment of lung morphology, parenchymal density, and pulmonary PBV. In patients with pulmonary emphysema, automated quantification of pulmonary PBV in DE-CTPA can be used for a quick, reader-independent estimation of global and regional pulmonary perfusion, which correlates with several lung function parameters
Strong Deformation Effects in Hot Rotating 46Ti
Exotic-deformation effects in 46Ti nucleus were investigated by analysing the
high-energy gamma-ray and the alpha-particle energy spectra. One of the
experiments was performed using the charged-particle multi-detector array ICARE
together with a large volume (4"x4") BGO detector. The study focused on
simultaneous measurement of light charged particles and gamma-rays in
coincidence with the evaporation residues. The experimental data show a
signature of very large deformations of the compound nucleus in the Jacobi
transition region at the highest spins. These results are compared to data from
previous experiments performed with the HECTOR array coupled to the EUROBALL
array, where it was found that the GDR strength function is highly fragmented,
strongly indicating a presence of nuclei with very large deformation.Comment: 10 pages, 6 figures, Proceedings of the Zakopane Conference on
Nuclear Physics, to be published in Acta Phys. Pol. B (2007
Quantum singularity of Levi-Civita spacetimes
Quantum singularities in general relativistic spacetimes are determined by
the behavior of quantum test particles. A static spacetime is quantum
mechanically singular if the spatial portion of the wave operator is not
essentially self-adjoint. Here Weyl's limit point-limit circle criterion is
used to determine whether a wave operator is essentially self-adjoint. This
test is then applied to scalar wave packets in Levi-Civita spacetimes to help
elucidate the physical properties of the spacetimes in terms of their metric
parameters
Measurement of gamma p --> K+ Lambda and gamma p --> K+ Sigma0 at photon energies up to 2.6 GeV
The reactions gamma p --> K+ Lambda and gamma p --> K+ Sigma0 were measured
in the energy range from threshold up to a photon energy of 2.6 GeV. The data
were taken with the SAPHIR detector at the electron stretcher facility, ELSA.
Results on cross sections and hyperon polarizations are presented as a function
of kaon production angle and photon energy. The total cross section for Lambda
production rises steeply with energy close to threshold, whereas the Sigma0
cross section rises slowly to a maximum at about E_gamma = 1.45 GeV. Cross
sections together with their angular decompositions into Legendre polynomials
suggest contributions from resonance production for both reactions. In general,
the induced polarization of Lambda has negative values in the kaon forward
direction and positive values in the backward direction. The magnitude varies
with energy. The polarization of Sigma0 follows a similar angular and energy
dependence as that of Lambda, but with opposite sign.Comment: 21 pages, 25 figures, submitted to Eur. Phys. J.
Loop quantum gravity: the first twenty five years
This is a review paper invited by the journal "Classical ad Quantum Gravity"
for a "Cluster Issue" on approaches to quantum gravity. I give a synthetic
presentation of loop gravity. I spell-out the aims of the theory and compare
the results obtained with the initial hopes that motivated the early interest
in this research direction. I give my own perspective on the status of the
program and attempt of a critical evaluation of its successes and limits.Comment: 24 pages, 3 figure
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