Kejadian Penyakit Toxocariasis pada Pasien Anjing dan Kucing di Klinik Hewan Jogja Periode 2019-2020

Latar Belakang: Toxocariasis merupakan penyakit infeksi yang sering menyerang pada hewan kesayangan anjing kucing disebabkan oleh cacing Toxocara sp. Penyakit ini termasuk dalam golongan penyakit zoonosis karena dapat menular pada hewan dan pada manusia dapat menyebabkan visceral larval migrans. Tujuan penelitian ini adalah untuk mengetahui angka kejadian penyakit zoonotik Toxocariasis pada pasien anjing dan kucing yang ditangani di Klinik Hewan Jogja selama masa pandemi Covid-19 tahun 2019-2020. Hasil penelitian diharapkan dapat memberikan gambaran angka kejadian penyakit zoonosis Toxocariasis pada anjing kucing, yang selanjutnya akan sangat bermanfaat untuk dasar edukasi ke masyarakat luas untuk pencegahan atau antisipasi penyakit zoonosis tersebut. Metode: Penelitian dilakukan dengan wawancara, dan melakukan pemeriksaan secara langsung pada pasien anjing kucing yang terindikasi menderita cacingan yang ditangani di KHJ selama covid-19 tahun 2019-2020. Selanjutnya dilakukan anamnesa, pemeriksaan fisik, pemeriksaan laboratorium feses, dan penanganan pasien. Apabila ditemukan telur cacing Toxocara sp pada pemeriksaan mikroskopis feses, maka pasien dinyatakan positif menderita Toxocariasis. Penelitian juga memanfaatkan data medical record yang ada di Klinik Hewan Jogja. Hasil data yang diperoleh, selanjutnya dicatat, disajikan dalam bentuk tabel, dan dianalisis secara diskriptif. Hasil: Berdasar pada hasil penelitian menunjukkan bahwa angka kejadian penyakit zoonosis Toxocariasis tahun 2019 dan 2020 adalah pada pada anjing 1,60% dan 1,58%, dan pada kucing 4,30% dan 6,66%.  Hasil penelitian menunjukkan bahwa selama covid-19 sepanjang tahun 2019-2020 Januari sampai Desember di Klinik Hewan Jogja selalu menangani pasien anjing dan kucing  yang menderita Toxocariasis.Simpulan Hasil penelitian memberikan gambaran bahwa kejadian penyakit zoonosis Toxocariasis selalu ada di masyarakat, sehingga edukasi ke masyarakat luas untuk pencegahan atau antisipasi penyakit zoonosis tersebut harus terus dilakukan, sehingga masyarakat sehat terbebas dari penyakit zoonosis Toxocariasis

Stability of gag-ca gene of Jembrana Virus in pcDNA-ca recombinant plasmid vector in Escherichia coli DH5α which has been stored for eight years

The aim of this study was to determine the stability of gag-ca gene in a pcDNA-ca recombinant plasmid vector in Escherichia coli DH5α which has been stored for eight years using Polymerase Chain Reaction method (PCR). Gag-ca gene which codes mayor protein has been characterized as immunodominan antigen, and it also has positive reaction to animal antibody which infected by Jembrana virus, so it usually used as vaccine resources and serologic detection for Jembrana disease. The research was done by analyzing gag-ca gene of Jembrana virus in pcDNA-ca recombinant plasmid in Escherichia coli DH5α which has been stored for eightyears. The pcDNA-ca recombinant plasmid was purified from cultured Escherichia coli DH5α recombinant bacteria using "High Pure Plasmid Isolation Kit", then amplificated with “Pure Taq-Ready To Go PCR Kit” using specific primers. PCR products showed a positive result that pcDNA-ca recombinant plasmid contains gag-ca gene. PCR products identification using electrophoresis on agarose gel 1% showed that DNA stand apparently carried gag-ca gene at approximate amplicon 210 base pair (bp)

Representation of Various Systematic Disruptions within the Patients Handled at “Klinik Hewan Jogja” between January and June 2018

Various diseases or disruption could affect all of the patients handled at any clinic or hospital. The health problems would be varied, depending on the disease or systemic disruption that ailed the patient. This research aims to figure out the spread of said health problems in the patients handled at Klinik Hewan Jogja between January and June 2018. A numerical representation of afflictions that ails the patients in a clinic or a hospital during a period of time would provide the information needed to anticipate several diseases, facilitate easier treatment and follow-up actions for certain ailments, offer prevention methods, and lastly to yield data for clients' education. The data gathered is also vital to give a general picture the problems that frequent a certain area, thus imparting important information to all other clinics or hospitals in the said region

Proceedings of the 3rd International Conference on Community Engagement and Education for Sustainable Development

This proceeding contains articles on the various ideas of the academic community presented at The 3rd International Conference on Community Engagement and Education for Sustainable Development (ICCEESD 2022) organized by the Universitas Gadjah Mada, Indonesia on 7th-8th December 2022.  ICCEESD is a biannual forum for sharing, benchmarking, and discussing HEI’s activities in developing Education for Sustainable Development towards community engagement. Education for Sustainability as a teaching strategy for resolving community challenges through formal, informal, or non-formal education is expected to benefit from various community service best practices by academics, researchers, and students. The 3rd ICCEESD has “Strengthening Education for Sustainability Towards Better Community Engagement” as its theme this year. It is expected that the 3rd ICCEESD will provide a forum for the presenters and participants to exchange best practices, policies, and conceptual implementation of Education for Sustainability towards better community engagement and explore ideas to address community needs.  Conference Title: 3rd International Conference on Community Engagement and Education for Sustainable DevelopmentConference Theme: Strengthening Education for Sustainability Towards Better Community EngagementConference Acronyms: ICCEESD 2022Conference Date: 7th-8th December 2022Conference Location: Grand Rohan Jogja Yogyakarta, IndonesiaConference Organizer: Universitas Gadjah Mada, Indonesi