Pemanfaatan Limbah Kayu melalui Puzzle Wayang sebagai Media Pengenalan Budaya untuk Anak Usia Dini

Pengolahan limbah di jepara sebagai kota ukir masih sangat kurang, kesalahan dalam mengelola akan menyebabkan berbahaya bagi lingkungan dan masyarakat, sehingga menyebabkan kerusakan lingkungan contoh pencemaran lingkungan. Selama ini limbah pabrik mebel mempunyai potensi untuk dikembangkan menjadi kerajinan tangan yang bernilai jual salah satunya alat permainan anak. Sasaran program pengabdian ini ada dua mitra yaitu : Mitra yang produktif yaitu PT ERNALIM. Mitra ini membantu tim pelaksana untuk mengolah limbah dari meubel ukir. Peserta dari mitra pertama yaitu 4 orang karyawan tersebut. Untuk memanfaatkan limbah kayu ini kami mengajak mitra tersebut untuk membuat alat permainan anak-anak berupa puzzle. Mitra yang kedua adalah mitra sebagai bentuk kelanjutan pemanfaatan limbah yaitu TK TARBIYATUL ATHFAL Muslimat NU di Pecangaan Jepara. Puzzle ini berbentuk wayang yaitu punakawan terdiri dari semar, gareng, pethruk dan bagong. Penyuluhan game edukasi ini diikuiti oleh guru dan anak-anak TK Tarbiyatul Athfal. Mereka sangat antusias dalam kegiatan penyuluhan ini mengingat puzzle yang digunakan adalah bukan biasanya. Dari Tim ini mengenalkan budaya Indonesia sejak dini melalui game puzzle berbentuk wayang. Kegiatan ini memberikan kontribusi pada dunia pendidikan untuk meningkatkan kreativitas ketrampilan dalam memecahkan masalah menyusun gambar

Neocortical Tet3-mediated accumulation of 5-hydroxymethylcytosine promotes rapid behavioral adaptation

5-hydroxymethylcytosine (5-hmC) is a novel DNA modification that is highly enriched in the adult brain and dynamically regulated by neural activity. 5-hmC accumulates across the lifespan; however, the functional relevance of this change in 5-hmC and whether it is necessary for behavioral adaptation have not been fully elucidated. Moreover, although the ten-eleven translocation (Tet) family of enzymes is known to be essential for converting methylated DNA to 5-hmC, the role of individual Tet proteins in the adult cortex remains unclear. Using 5-hmC capture together with high-throughput DNA sequencing on individual mice, we show that fear extinction, an important form of reversal learning, leads to a dramatic genome-wide redistribution of 5-hmC within the infralimbic prefrontal cortex. Moreover, extinction learning-induced Tet3-mediated accumulation of 5-hmC is associated with the establishment of epigenetic states that promote gene expression and rapid behavioral adaptation

GTF2IRD2 from the Williams-Beuren critical region encodes a mobile-element-derived fusion protein that antagonizes the action of its related family members

GTF2IRD2 belongs to a family of transcriptional regulators (including TFII-I and GTF2IRD1) that are responsible for many of the key features of Williams-Beuren syndrome (WBS). Sequence evidence suggests that GTF2IRD2 arose in eutherian mammals by duplication and divergence from the gene encoding TFII-I. However, in GTF2IRD2, most of the C-terminal domain has been lost and replaced by the domesticated remnant of an in-frame hAT-transposon mobile element. In this first experimental analysis of function, we show that transgenic expression of each of the three family members in skeletal muscle causes significant fiber type shifts, but the GTF2IRD2 protein causes an extreme shift in the opposite direction to the two other family members. Mating of GTF2IRD1 and GTF2IRD2 mice restores the fiber type balance, indicating an antagonistic relationship between these two paralogs. In cells, GTF2IRD2 localizes to cytoplasmic microtubules and discrete speckles in the nuclear periphery. We show that it can interact directly with TFII-IΒ and GTF2IRD1, and upon co-transfection changes the normal distribution of these two proteins into a punctate nuclear pattern typical of GTF2IRD2. These data suggest that GTF2IRD2 has evolved as a regulator of GTF2IRD1 and TFII-I; inhibiting their function by direct interaction and sequestration into inactive nuclear zones