3 research outputs found
Vergleich der miRNA-Expressionsmuster in Primärtumoren und Fernmetastasen des klarzelligen Nierenzellkarzinoms
Das NZK ist der dritthäufigste Tumor des Urogenitaltraktes und das kzNZK mit 75% der häufigste Subtyp des NZKs. Patienten mit einem NZK besitzen zu 30% bereits zum Diagnosezeitpunkt Metastasen und 30-50% entwickeln im weiteren Verlauf Metastasen. Die Metastasierung stellt den wichtigsten prognoselimitierenden Faktor dar. Um das individuelle Metastasierungsrisiko bestimmen zu können, sind bisher keine hinreichenden klinischen und histopathologischen Parameter vorhanden. MiRNAs sind nicht kodierende einzelsträngige RNA-Moleküle mit einer Länge von ca. 22 nt. Den miRNAs wird eine wichtige Rolle bei der Tumorentstehung, dem -progress und für die Metastasierung zugesprochen. Somit sind miRNAs potentielle Biomarker für Diagnostik und Prognosebewertung von Tumoren. Zu Beginn der Promotionsarbeit war für verschiedene Karzinome die metastasierungsassoziierte Funktion von miRNAs bereits belegt. Allerdings lagen für das NZK zur Bedeutung von miRNAs im Prozess der Metastasierung wenige Daten vor. Es konnte gezeigt werden, dass die Expression von verschiedenen miRNAs in metastasierten und nicht metastasierten Primärtumoren different ist. Allerdings wurden die Expressionsprofile von miRNAs nicht in Fernmetastasen untersucht, sodass das Ziel der vorliegenden Arbeit darin bestand, die Hauptmetastasierungsorte hinsichtlich der miRNA-Expression spezifischer miRNAs zu untersuchen und ortsspezifische Gemeinsamkeiten und Unterschiede zu explorieren, um somit ein besseres Verständnis über den Prozess der Metastasierung zu gewinnen
Pseudo aneurysm of the uterine artery with arteriovenous fistula after cesarean section: A rare but sinister cause of delayed postpartum hemorrhage
Pseudoaneurysm of the uterine artery is a rare complication of cesarean section. Delayed diagnosis and management may result in rapid and catastrophic postpartum hemorrhage and may necessitate hysterectomy or can even be fatal. A 28-year-old woman (gravida I, para I) presented with delayed postpartum hemorrhage 10 days after emergency cesarean section. Using transabdominal and transvaginal Color Doppler sonography, we detected a pseudoaneurysm in the posterior uterine wall, which was successfully treated with selective embolization distal and proximal to the lesion with platinum coils. In patients with delayed postpartum hemorrhage-especially after cesarean section-the rare possibility of uterine artery pseudoaneurysm must be kept in mind
MicroRNAs with Prognostic Potential for Metastasis in Clear Cell Renal Cell Carcinoma: A Comparison of Primary Tumors and Distant Metastases
Background: MicroRNAs (miRNAs) are regulators of gene expression in tumor development and progression. However, their influence on metastasis of clear cell renal cell carcinoma (ccRCC) is less understood. To determine the role of miRNAs in metastatic progression, miRNA expression in primary ccRCC was compared to distant metastases. Methods: Total RNA of 53 primary ccRCCs, 35 distant metastases from lung, bone, brain, and abdomen, as well as 17 normal kidney tissues was isolated from fresh frozen tissue and formalin-fixed paraffin-embedded (FFPE) samples. The miRNA microarrays were performed based on fresh frozen tissue. Results were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on fresh frozen tissue and FFPE samples. Real-time cell analyses and transwell invasion assays were carried out after transient transfection of microRNA-30c (miR-30c) in cell line 786-O. Results: There were 14 miRNAs differently expressed in metastatic primary ccRCC and distant metastases compared to non-metastatic primary tumors. A strong correlation of miRNAs to progression-free- and cancer-specific 5-year-survival was determined. Specific miRNAs were differently expressed in distant metastases compared to primary ccRCC. A miRNA signature distinguished lung metastases from other metastatic sites. Overexpression of miR-30c increased adherence and decreased migration and invasion in the ccRCC cell line. Conclusions: MiRNAs are deregulated in metastatic primary ccRCC and could be promising prognostic markers for an early prediction of metastasis. Alterations in miRNA expression characterize distant metastases of different metastatic sites. Furthermore, our study suggests a functional role of miR-30c in metastasis. The miRNAs could be a helpful tool for individual follow-up prediction and personalized therapy selection. © 2013 Society of Surgical Oncology