19 research outputs found
“Patch”ing Up Our Tumor Signaling Knowledge
The tumor suppressor Patched1 (Ptch1) possesses well-described roles in regulating sonic hedgehog (SHH) signaling in the skin and preventing the formation of basal cell carcinomas (BCCs). In this issue, Kang et al. extend their previous work to show that a naturally occurring allele of Ptch1 found in FVB mice promotes early squamous cell carcinoma (SCC) growth without aberrant activation of the SHH pathway. The study reveals new roles for Ptch1 that lie at the nexus between BCC and SCC formation
Epigenetic targeting of Hedgehog pathway transcriptional output through BET bromodomain inhibition
Hedgehog signaling drives oncogenesis in several cancers and strategies targeting this pathway have been developed, most notably through inhibition of Smoothened. However, resistance to Smoothened inhibitors occurs via genetic changes of Smoothened or other downstream Hedgehog components. Here, we overcome these resistance mechanisms by modulating GLI transcription via inhibition of BET bromodomain proteins. We show the BET bromodomain protein, BRD4, regulates GLI transcription downstream of SMO and SUFU and chromatin immunoprecipitation studies reveal BRD4 directly occupies GLI1 and GLI2 promoters, with a substantial decrease in engagement of these sites upon treatment with JQ1, a small molecule inhibitor targeting BRD4. Globally, genes associated with medulloblastoma-specific GLI1 binding sites are downregulated in response to JQ1 treatment, supporting direct regulation of GLI activity by BRD4. Notably, patient- and GEMM-derived Hedgehog-driven tumors (basal cell carcinoma, medulloblastoma and atypical teratoid/rhabdoid tumor) respond to JQ1 even when harboring genetic lesions rendering them resistant to Smoothened antagonists
Noncanonical hedgehog pathway activation through SRF–MKL1 promotes drug resistance in basal cell carcinomas
Advanced treatment for basal cell carcinomas.
Basal cell carcinomas (BCCs) are very common epithelial cancers that depend on the Hedgehog pathway for tumor growth. Traditional therapies such as surgical excision are effective for most patients with sporadic BCC; however, better treatment options are needed for cosmetically sensitive or advanced and metastatic BCC. The first approved Hedgehog antagonist targeting the membrane receptor Smoothened, vismodegib, shows remarkable effectiveness on both syndromic and nonsyndromic BCCs. However, drug-resistant tumors frequently develop, illustrating the need for the development of next-generation Hedgehog antagonists targeting pathway components downstream from Smoothened. In this article, we will summarize available BCC treatment options and discuss the development of next-generation antagonists
Tumor-Derived Suppressor of Fused Mutations Reveal Hedgehog Pathway Interactions
<div><p>The Hedgehog pathway is a potent regulator of cellular growth and plays a central role in the development of many cancers including basal cell carcinoma (BCC). The majority of BCCs arise from mutations in the Patched receptor resulting in constitutive activation of the Hedgehog pathway. Secondary driver mutations promote BCC oncogenesis and occur frequently due to the high mutational burden resulting from sun exposure of the skin. Here, we uncover novel secondary mutations in Suppressor of Fused (SUFU), the major negative regulator of the Hedgehog pathway. SUFU normally binds to a Hedgehog transcriptional activator, GLI1, in order to prevent it from initiating transcription of Hedgehog target genes. We sequenced tumor-normal pairs from patients with early sporadic BCCs. This resulted in the discovery of nine mutations in SUFU, which were functionally investigated to determine whether they help drive BCC formation. Our results show that four of the SUFU mutations inappropriately activate the Hedgehog pathway, suggesting they may act as driver mutations for BCC development. Indeed, all four of the loss of function SUFU variants were found to disrupt its binding to GLI, leading to constitutive pathway activation. Our results from functional characterization of these mutations shed light on SUFU’s role in Hedgehog signaling, tumor progression, and highlight a way in which BCCs can arise.</p></div
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AP-1 and TGFĂź cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.
Tumor heterogeneity and lack of knowledge about resistant cell states remain a barrier to targeted cancer therapies. Basal cell carcinomas (BCCs) depend on Hedgehog (Hh)/Gli signaling, but can develop mechanisms of Smoothened (SMO) inhibitor resistance. We previously identified a nuclear myocardin-related transcription factor (nMRTF) resistance pathway that amplifies noncanonical Gli1 activity, but characteristics and drivers of the nMRTF cell state remain unknown. Here, we use single cell RNA-sequencing of patient tumors to identify three prognostic surface markers (LYPD3, TACSTD2, and LY6D) which correlate with nMRTF and resistance to SMO inhibitors. The nMRTF cell state resembles transit-amplifying cells of the hair follicle matrix, with AP-1 and TGFĂź cooperativity driving nMRTF activation. JNK/AP-1 signaling commissions chromatin accessibility and Smad3 DNA binding leading to a transcriptional program of RhoGEFs that facilitate nMRTF activity. Importantly, small molecule AP-1 inhibitors selectively target LYPD3+/TACSTD2+/LY6D+ nMRTF human BCCs ex vivo, opening an avenue for improving combinatorial therapies
Select SUFU variants disrupt binding to GLI1.
<p>(A). HEK-293T cells were co-transfected with SUFU:TAP and GFP:GLI and co-immunoprecipitated to determine interaction between the two proteins. All of the truncation mutations (146X, 199X, and 464X) fully abolished any interaction to GLI1, while one point mutation (E376K) disrupted 94.9% of SUFU’s binding to GLI1, as shown by the quantification below the GLI1 blot. Asterisks indicate the correct SUFU band on the input blot. (B). Comparative growth assay in ASZ001 cells nucleofected with SUFU WT and SUFU variants. (C). E376K visualized on the known GLI-SUFU crystal structure, revealing that E376 forms a hydrogen bond to GLI1 in the pocket between SUFU’s lobes. Blue is GLI1, green is E376K, and the dashed red line is the hydrogen bond.</p
SUFU variants in basal cell carcinoma.
<p>Whole exome sequencing with targeted re-sequencing of sporadic and Gorlin’s syndrome tumor-normal pairs revealed 9 variants, depicted in black on the SUFU gene map. Gorlin’s mutations are shown in green, while sporadic mutations are in black. Red variants are previously known SUFU mutations that disrupt SUFU-GLI1 interactions [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0168031#pone.0168031.ref012" target="_blank">12</a>]. Highlighted purple regions are GLI1 binding sites. 120–125 binds the C-terminus of GLI1 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0168031#pone.0168031.ref012" target="_blank">12</a>], while 388–398 binds the N-terminus of GLI1[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0168031#pone.0168031.ref004" target="_blank">4</a>].</p
SUFU variants show differential suppression of HH signaling.
<p>(A). <i>Sufu</i>-null MEFs nucleofected with either GFP or wild type SUFU:GFP, <i>Gli1</i> mRNA is used as a readout of Hh pathway activity. (B). qRTPCR used to determine the activity of each SUFU variant, with a Western blot shown directly above to show that each protein is comparably expressed. SUFU:GFP appears as expected at 81 kDa, with truncations 146X and 199X appearing at 48 kDa and 53 kDa, respectively. Gapdh is a loading control. Asterisks denote the correct band on the GFP blot. * is P<0.005, ** is P<0.0005, *** is P<0.0001. Significance was determined by using an unpaired t test with equal SD, comparing each mutant to wild type. P values were calculated with a two-tailed comparison.</p