Frequency control in synchronized networks of inhibitory neurons

We analyze the control of frequency for a synchronized inhibitory neuronal network. The analysis is done for a reduced membrane model with a biophysically-based synaptic influence. We argue that such a reduced model can quantitatively capture the frequency behavior of a larger class of neuronal models. We show that in different parameter regimes, the network frequency depends in different ways on the intrinsic and synaptic time constants. Only in one portion of the parameter space, called `phasic', is the network period proportional to the synaptic decay time. These results are discussed in connection with previous work of the authors, which showed that for mildly heterogeneous networks, the synchrony breaks down, but coherence is preserved much more for systems in the phasic regime than in the other regimes. These results imply that for mildly heterogeneous networks, the existence of a coherent rhythm implies a linear dependence of the network period on synaptic decay time, and a much weaker dependence on the drive to the cells. We give experimental evidence for this conclusion.Comment: 18 pages, 3 figures, Kluwer.sty. J. Comp. Neurosci. (in press). Originally submitted to the neuro-sys archive which was never publicly announced (was 9803001

A Systems Approach to the Physiology of Weightlessness

A systems approach to the unraveling of the complex response pattern of the human subjected to weightlessness is presented. The major goal of this research is to obtain an understanding of the role that each of the major components of the human system plays following the transition to and from space. The cornerstone of this approach is the utilization of a variety of mathematical models in order to pose and test alternative hypotheses concerned with the adaptation process. An integrated hypothesis for the human physiological response to weightlessness is developed

Measuring Molecular, Neutral Atomic, and Warm Ionized Galactic Gas Through X-Ray Absorption

We study the column densities of neutral atomic, molecular, and warm ionized Galactic gas through their continuous absorption of extragalactic X-ray spectra at |b| > 25 degrees. For N(H,21cm) < 5x10^20 cm^-2 there is an extremely tight relationship between N(H,21cm) and the X-ray absorption column, N(xray), with a mean ratio along 26 lines of sight of N(xray)/N(H,21cm) = 0.972 +- 0.022. This is significantly less than the anticpated ratio of 1.23, which would occur if He were half He I and half He II in the warm ionized component. We suggest that the ionized component out of the plane is highly ionized, with He being mainly He II and He III. In the limiting case that H is entirely HI, we place an upper limit on the He abundance in the ISM of He/H <= 0.103. At column densities N(xray) > 5x10^20 cm^-2, which occurs at our lower latitudes, the X-ray absorption column N(xray) is nearly double N(H,21cm). This excess column cannot be due to the warm ionized component, even if He were entirely He I, so it must be due to a molecular component. This result implies that for lines of sight out of the plane with |b| ~ 30 degrees, molecular gas is common and with a column density comprable to N(H,21cm). This work bears upon the far infrared background, since a warm ionized component, anticorrelated with N(H,21cm), might produce such a background. Not only is such an anticorrelation absent, but if the dust is destroyed in the warm ionized gas, the far infrared background may be slightly larger than that deduced by Puget et al. (1996).Comment: 1 AASTeX file, 14 PostScript figure files which are linked within the TeX fil

Small-Scale structure in the Galactic ISM: Implications for Galaxy Cluster Studies

Observations of extragalactic objects need to be corrected for Galactic absorption and this is often accomplished by using the measured 21 cm HI column. However, within the beam of the radio telescope there are variations in the HI column that can have important effects in interpreting absorption line studies and X-ray spectra at the softest energies. We examine the HI and DIRBE/IRAS data for lines of sight out of the Galaxy, which show evidence for HI variations in of up to a factor of three in 1 degree fields. Column density enhancements would preferentially absorb soft X-rays in spatially extended objects and we find evidence for this effect in the ROSAT PSPC observations of two bright clusters of galaxies, Abell 119 and Abell 2142. For clusters of galaxies, the failure to include column density fluctuations will lead to systematically incorrect fits to the X-ray data in the sense that there will appear to be a very soft X-ray excess. This may be one cause of the soft X-ray excess in clusters, since the magnitude of the effect is comparable to the observed values.Comment: 16 pages, 9 figures, to appear in the Astrophysical Journal, vol. 597 (1 Nov 2003

Urban grasslands support threatened water voles

Urbanisation is often linked with habitat loss and a reduction in species richness but some species may be able to adapt to urban environments. Water voles Arvicola amphibius, a rapidly declining species in the UK, have recently been recorded in isolated grassland habitats in Glasgow, Scotland’s largest city (human population 1.2 million). The aim of this study was to determine the distribution and habitat characteristics of water vole populations occupying these dry grasslands. Field work was undertaken from March to October 2014 in a 34 km2 study area located 3 km east of the city centre. Field sign transects recorded water vole presence in 21/65 (32%) and 19/62 (31%) surveyed sites in spring and autumn, respectively. Vole occupancy increased with distance from water and was greatest in parkland, followed by sites with rank vegetation and roadside habitats. Occupancy was lower where signs of predators were recorded but surprisingly occupancy was found to be greater in the most disturbed sites, perhaps linked to the fact that many of these sites were public parks containing suitable grassland. Sites occupied by water voles were classed as neutral grasslands with species composition dominated by two main species. The number of grassland sites occupied by water voles, especially within public areas suggests that careful management of these urban grassland habitats will benefit the conservation of this highly threatened species in the UK

Evaluation of a novel assay for detection of the fetal marker RASSF1A: facilitating improved diagnostic reliability of noninvasive prenatal diagnosis

BackgroundAnalysis of cell free fetal (cff) DNA in maternal plasma is used routinely for non invasive prenatal diagnosis (NIPD) of fetal sex determination, fetal rhesus D status and some single gene disorders. True positive results rely on detection of the fetal target being analysed. No amplification of the target may be interpreted either as a true negative result or a false negative result due to the absence or very low levels of cffDNA. The hypermethylated RASSF1A promoter has been reported as a universal fetal marker to confirm the presence of cffDNA. Using methylation-sensitive restriction enzymes hypomethylated maternal sequences are digested leaving hypermethylated fetal sequences detectable. Complete digestion of maternal sequences is required to eliminate false positive results.MethodscfDNA was extracted from maternal plasma (n = 90) and digested with methylation-sensitive and insensitive restriction enzymes. Analysis of RASSF1A, SRY and DYS14 was performed by real-time PCR.ResultsHypermethylated RASSF1A was amplified for 79 samples (88%) indicating the presence of cffDNA. SRY real time PCR results and fetal sex at delivery were 100% accurate. Eleven samples (12%) had no detectable hypermethylated RASSF1A and 10 of these (91%) had gestational ages less than 7 weeks 2 days. Six of these samples were male at delivery, five had inconclusive results for SRY analysis and one sample had no amplifiable SRY.ConclusionUse of this assay for the detection of hypermethylated RASSF1A as a universal fetal marker has the potential to improve the diagnostic reliability of NIPD for fetal sex determination and single gene disorders