The establishment of two novel bovine cell lines by transfection with the putative transforming region of bovine adenovirus type 3

Human adenoviruses (Ads), members of the family adenoviridae, are medium-sized DNA viruses which have been used as valuable research tools for the study of RNA processing, oncogenic transformation, and for the development of viral vectors for use in gene delivery and immunization technology. The left 12% of the linear Ad genollle codes for products which are necessary for the efficient replication of the virus, as well as being responsible for the forlllation of tumors in animallllodels. The establishlllent of the 293 cell line, by immortalization of human embryonic kidney cells with th~ E1 region of Ad type S (AdS), has facilitated extensive manipulation of the Ads and the development of recombinant Ad vectors. The study of bovine adenoviruses (BAVs), which cause mild respiratory and gastrointestinal infections in cattle has, on the other hand, been limited primarily to that of infectivity, immunology and clinicallllanifestations. As a result, any potential as gene delivery vehicles has not yet been realized. Continued research into the molecular biolo~gy of BAVs and the development of recolllbinant vectors would benefit from the development of a cell line analogous to that of the 293 cells. In an attelllpt to establish such a cell line, the recombinant plaslllid pKC-neo was constructed, containing the left 0-19.7% of the BAV type 3 (BAV3) genome, and the selectable marker for resistance to the aminoglycoside G418, a neomycin derivative. The plasmid construct was then used to transfect both the Madin-Darby bovine kidney (MDBK) -iicell line and primary bovine lung cells, after which G418-resistant foci were selected for analysis. Two cell lines, E61 (MDBK) and E24 (primary lung), were subsequently selected and analysed for DNA content, revealing the presence of the pKC-neo sequences in their respective genomes. In addition, BAV3 RNA transcripts were detected in the E61 cells. Although the presence of E1 products has yet to be confirmed in both cell lines, the E24 cells exhibit a phenotype characteristic of partial transformation by E1. The apparent immortalization of the primary lung cells will permit exploitation of their ability to take up exogenous DNA at high efficiency

Oil and the Porcupine Caribou Herd — Can we quantify the impacts?

We report on a number of ongoing studies and discuss what projects are in place to further refine and test relationships

Plant Diversity, Soil Microbial Communities, And Ecosystem Function: Are There Any Links?

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/117152/1/ecy20038482042.pd

Condensation Coefficient Measurement for Water in the UMR Cloud Simulation Chamber

A systematic series of condensation coefficient measurements of water have been made using the University of Missouri-Rolla cooled-wall expansion chamber which simulates the thermodynamics of cloud. This coefficient is seen to decrease from a value near unity, at the outset of simulation, to a value in the neighborhood of 0.01 toward the end of a simulation. Final values of this coefficient are sufficiently low as to contribute significantly to the broadening of the drop-size distribution in cloud

Field relations, age, and tectonic setting of metamorphic and plutonic rocks in the Creignish Hills – North Mountain area, southwestern Cape Breton Island, Nova Scotia, Canada

 The Creignish Hills and North Mountain areas of southwestern Cape Breton Island consist mostly of Neoproterozoic rocks typical of the Ganderian Bras d’Or terrane. U-Pb ages presented here for detrital zircon in the Blues Brook Formation of the Creignish Hills confirm a depositional age no greater than about 600 Ma. Although it is possible that some components of the formation are much older, similarities in rock types and field relations suggest that this is not the case. It is likely that the equivalent Malagawatch Formation of the North Mountain area, as well as high-grade metasedimentary rocks of the Melford Formation and Chuggin Road complex in the Creignish Hills and Lime Hill gneiss complex in the North Mountain area, represent the same or stratigraphically equivalent units as the Blues Brook Formation. The minimum ages of all of these units are constrained by cross-cutting syn- and post-tectonic plutons with ages mostly between 565 and 550 Ma, indicating that sediments were deposited, regionally metamorphosed, deformed, and intruded by plutons in less than 40–50 million years. The assemblage of pelitic, psammitic, and carbonate rocks indicates that a passive margin in a tropical climate was quickly changed to an active Andean-type continental margin in which voluminous calcalkaline dioritic to granitic plutons were emplaced. This sedimentary and tectonic history is characteristic of the Bras d’Or terrane and is shared by its likely correlative, the Brookville terrane in southern New Brunswick.

SDSS J092455.87+021924.9: an Interesting Gravitationally Lensed Quasar from the Sloan Digital Sky Survey

We report the discovery of a new gravitationally lensed quasar from the Sloan Digital Sky Survey, SDSS J092455.87+021924.9 (SDSS J0924+0219). This object was selected from among known SDSS quasars by an algorithm that was designed to select another known SDSS lensed quasar (SDSS 1226-0006A,B). Five separate components, three of which are unresolved, are identified in photometric follow-up observations obtained with the Magellan Consortium's 6.5m Walter Baade telescope at Las Campanas Observatory. Two of the unresolved components (designated A and B) are confirmed to be quasars with z=1.524; the velocity difference is less than 100 km sec^{-1} according to spectra taken with the W. M. Keck Observatory's Keck II telescope on Mauna Kea. A third stellar component, designated C, has the colors of a quasar with redshift similar to components A and B. The maximum separation of the point sources is 1.78". The other two sources, designated G and D, are resolved. Component G appears to be the best candidate for the lensing galaxy. Although component D is near the expected position of the fourth lensed component in a four image lens system, its properties are not consistent with being the image of a quasar at z~1.5. Nevertheless, the identical redshifts of components A and B and the presence of component C strongly suggest that this object is a gravitational lens. Our observations support the idea that a foreground object reddens the fourth lensed component and that another unmodeled effect (such as micro- or milli-lensing) demagnificates it, but we cannot rule out the possibility that SDSS0924+0219 is an example of the relatively rare class of ``three component'' lens systems.Comment: 24 pages, 6 figures, accepted by A

The clustering of massive galaxies at z~0.5 from the first semester of BOSS data

We calculate the real- and redshift-space clustering of massive galaxies at z~0.5 using the first semester of data by the Baryon Oscillation Spectroscopic Survey (BOSS). We study the correlation functions of a sample of 44,000 massive galaxies in the redshift range 0.4<z<0.7. We present a halo-occupation distribution modeling of the clustering results and discuss the implications for the manner in which massive galaxies at z~0.5 occupy dark matter halos. The majority of our galaxies are central galaxies living in halos of mass 10^{13}Msun/h, but 10% are satellites living in halos 10 times more massive. These results are broadly in agreement with earlier investigations of massive galaxies at z~0.5. The inferred large-scale bias (b~2) and relatively high number density (nbar=3e-4 h^3 Mpc^{-3}) imply that BOSS galaxies are excellent tracers of large-scale structure, suggesting BOSS will enable a wide range of investigations on the distance scale, the growth of large-scale structure, massive galaxy evolution and other topics.Comment: 11 pages, 12 figures, matches version accepted by Ap

Five High-Redshift Quasars Discovered in Commissioning Imaging Data of the Sloan Digital Sky Survey

We report the discovery of five quasars with redshifts of 4.67 - 5.27 and z'-band magnitudes of 19.5-20.7 M_B ~ -27. All were originally selected as distant quasar candidates in optical/near-infrared photometry from the Sloan Digital Sky Survey (SDSS), and most were confirmed as probable high-redshift quasars by supplementing the SDSS data with J and K measurements. The quasars possess strong, broad Lyman-alpha emission lines, with the characteristic sharp cutoff on the blue side produced by Lyman-alpha forest absorption. Three quasars contain strong, broad absorption features, and one of them exhibits very strong N V emission. The amount of absorption produced by the Lyman-alpha forest increases toward higher redshift, and that in the z=5.27 object (D_A ~ 0.7) is consistent with a smooth extrapolation of the absorption seen in lower redshift quasars. The high luminosity of these objects relative to most other known objects at z >~ 5 makes them potentially valuable as probes of early quasar properties and of the intervening intergalactic medium.Comment: 13 pages in LaTex format, two postscirpt figures. Submitted to the Astronomical Journa

Changes in the Expression of Human Cell Division Autoantigen-1 Influence Toxoplasma gondii Growth and Development

Toxoplasma is a significant opportunistic pathogen in AIDS, and bradyzoite differentiation is the critical step in the pathogenesis of chronic infection. Bradyzoite development has an apparent tropism for cells and tissues of the central nervous system, suggesting the need for a specific molecular environment in the host cell, but it is unknown whether this environment is parasite directed or the result of molecular features specific to the host cell itself. We have determined that a trisubstituted pyrrole acts directly on human and murine host cells to slow tachyzoite replication and induce bradyzoite-specific gene expression in type II and III strain parasites but not type I strains. New mRNA synthesis in the host cell was required and indicates that novel host transcripts encode signals that were able to induce parasite development. We have applied multivariate microarray analyses to identify and correlate host gene expression with specific parasite phenotypes. Human cell division autoantigen-1 (CDA1) was identified in this analysis, and small interfering RNA knockdown of this gene demonstrated that CDA1 expression causes the inhibition of parasite replication that leads subsequently to the induction of bradyzoite differentiation. Overexpression of CDA1 alone was able to slow parasite growth and induce the expression of bradyzoite-specific proteins, and thus these results demonstrate that changes in host cell transcription can directly influence the molecular environment to enable bradyzoite development. Investigation of host biochemical pathways with respect to variation in strain type response will help provide an understanding of the link(s) between the molecular environment in the host cell and parasite development

A Survey of z>5.7 Quasars in the Sloan Digital Sky Survey II: Discovery of Three Additional Quasars at z>6

We present the discovery of three new quasars at z>6 in 1300 deg^2 of SDSS imaging data, J114816.64+525150.3 (z=6.43), J104845.05+463718.3 (z=6.23) and J163033.90+401209.6 (z=6.05). The first two objects have weak Ly alpha emission lines; their redshifts are determined from the positions of the Lyman break. They are only accurate to 0.05 and could be affected by the presence of broad absorption line systems. The last object has a Ly alpha strength more typical of lower redshift quasars. Based on a sample of six quasars at z>5.7 that cover 2870 deg^2 presented in this paper and in Paper I, we estimate the comoving density of luminous quasars at z 6 and M_{1450} < -26.8 to be (8 +/- 3)x10^{-10} Mpc^{-3} (for H_0 = 50 km/s/Mpc, Omega = 1). HST imaging of two z>5.7 quasars and high-resolution ground-based images (seeing 0.4'') of three additional z>5.7 quasars show that none of them is gravitationally lensed. The luminosity distribution of the high-redshfit quasar sample suggests the bright end slope of the quasar luminosity function at z 6 is shallower than Psi L^{-3.5} (2-sigma), consistent with the absence of strongly lensed objects.Comment: AJ in press (Apr 2003), 26 pages, 9 figure