294 research outputs found
Selection of imprinted nanoparticles by affinity chromatography
Soluble molecularly imprinted nanoparticles were synthesised via iniferter
initiated polymerisation and separated by size via gel permeation
chromatography. Subsequent fractionation of these particles by affinity
chromatography allowed the separation of high affinity fractions from the
mixture of nanoparticles. Fractions selected this way possess affinity similar
to that of natural antibodies (Kd 6.6 Ă 10â8) M and were also able to
discriminate between related functional analogues of the templ
The stabilisation of receptor structure in low cross-linked MIPs by an immobilised template
In molecularly imprinted polymers (MIPs) a high level of cross-linking is
usually important for preserving the receptor structure. We propose here an
alternative approach for stabilising binding sites, which involves the use of an
immobilised template. The idea is based on the assumption that an immobilised
template will ââholdââ polymeric chains and complementary functionalities
together, preventing the collapsing of the binding sites. To test this
postulate, a range of polymers was prepared using polymerisable (2,4-diamino-6-
(methacryloyloxy)ethyl-1,3,5-triazine) and non-polymerisable (or extractable)
(2,4-diamino-6-methyl-1,3,5-triazine) templates, methacrylic acid as functional
monomer and ethylene glycol dimethacrylate as cross-linker. The level of cross-
linking was varied from 12 to 80%. Polymerisations were performed in
acetonitrile using UV initiation. Binding properties of the synthesised
materials were characterised both by HPLC and equilibrium batch binding
experiments followed by HPLC-MS or UV-visible detection. The adsorption
isotherms of polymers were obtained and fitted to the Langmuir model to
calculate dissociation constant, Kd, and concentration of binding sites for each
material. The results strongly indicate that the presence of an immobilised
template improves the affinity of MIPs containing low percentages of cross-
linker. The low cross-linked MIPs synthesised with a polymerisable template also
retain a reasonable degree of selectivity. Low crosslinked MIPs with such
binding characteristics would be useful for the creation of new types of optical
and electrochemical sensors, where induced fit or the ââgate effectââ could be
used more effectively for generating and enhancin
The rational development of molecularly imprinted polymer-based sensors for protein detection.
The detection of specific proteins as biomarkers of disease, health status,
environmental monitoring, food quality, control of fermenters and civil defence
purposes means that biosensors for these targets will become increasingly more
important. Among the technologies used for building specific recognition
properties, molecularly imprinted polymers (MIPs) are attracting much attention.
In this critical review we describe many methods used for imprinting recognition
for protein targets in polymers and their incorporation with a number of
transducer platforms with the aim of identifying the most promising approaches
for the preparation of MIP-based protein sensors (277 references)
Better arthritis care: what training do community-based health professionals need to improve their care of people with arthritis? a Delphi study
Objective
The aim of the present study was to identify the competencies that non-specialist community-based nurses and allied health professionals (AHPs) need to enable them to assess, care for and manage arthritis appropriately.
Methods
A Delphi survey with an expert panel of 43 rheumatology specialists and expert patients was used to identify the competencies needed by community-based nurses and AHPs to enable them to improve their care of people with arthritis. The process was informed by feedback from focus groups with arthritis patients, community-based nurses and AHPs.
Results
The core competencies in arthritis care needed by non-specialist community-based nurses and AHPs were identified. The key goals identified were to increase the understanding of arthritis and its impact on patientsâ lives, and to increase the ability to help patients to self-manage their condition and access support. Competencies included an understanding of the pathology underlying inflammatory and non-inflammatory arthritis, the ability to distinguish between the two and the ability to recognize early warning signs, with an emphasis on osteoarthritis (OA), rheumatoid arthritis, gout and septic arthritis. Essential competencies included the ability to engage in shared decision making, goal setting and signposting, to provide patients with education and information and to make appropriate referrals.
Conclusions
Health professionals working in the community commonly encounter arthritis as a presenting problem or as a co-morbidity. The quality of care provided to people with inflammatory arthritis and OA in the community is currently variable. The present study identified the core competencies that all community-based nurses and AHPs should have in relation to OA and inflammatory arthritis
Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISAÂ - development of a novel assay for vancomycin
A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time. NanoMIPs were synthesized by a solid phase approach with immobilized vancomycin (template) and characterized using Biacore 3000, dynamic light scattering and electron microscopy. Immobilization, blocking and washing conditions were optimized in microplate format. The detection of vancomycin was achieved in competitive binding experiments with a HRP-vancomycin conjugate. The assay was capable of measuring vancomycin in buffer and in blood plasma within the range 0.001-70 nM with a detection limit of 0.0025 nM (2.5 pM). The sensitivity of the assay was three orders of magnitude better than a previously described ELISA based on antibodies. In these experiments nanoMIPs have shown high affinity and minimal interference from blood plasma components. Immobilized nanoMIPs were stored for 1 month at room temperature without any detrimental effects to their binding properties. The high affinity of nanoMIPs and the lack of a requirement for cold chain logistics make them an attractive alternative to traditional antibodies used in ELIS
A scoping review on occupational science research in European contexts
Background
A survey showed European occupational scientists cover a broad range in occupational science (OS) research, however, no contemporary overviews of European OS research exists, and current research may provide valuable information for OS and occupational therapy.
Aim
The aim was to provide an overview of contemporary European OS research.
Materials and method
A scoping review was performed, including studies conducted in Europe and published in the British Journal of Occupational Therapy (BJOT), the Scandinavian Journal of Occupational Therapy (SJOT) or the Journal of Occupational Science (JOS) between 2015 and 2020. The journals were systematically searched, and quality assessment and thematic analysis were undertaken.
Results
Findings from 93 articles identified many studies from the Nordic countries. Most studies applied qualitative research methods. Theoretical concepts from OS were used in data generating and discussions. A wide range of demographics, and living conditions were explored. Recent articles took a reflexive stance on the positionality of the researcher/s.
Conclusions
This review highlights the diversity of OS research, suggesting a solid theoretical knowledge base within European OS research.
Significance
The results contribute to further development and maturation of the discipline of OS in Europe and internationally
Introducing MINA-The Molecularly Imprinted Nanoparticles Assay
A new ELISAâ (enzymeâlinked immunosorbent assay)âlike assay is demonstrated in which no elements of biological origin are used for molecular recognition or signaling. Composite imprinted nanoparticles that contain a catalytic core and which are synthesized by using a solidâphase approach can simultaneously act as recognition/signaling elements, and be used with minimal modifications to standard assay protocols. This assay provides a new route towards replacement of unstable biomolecules in immunoassays
Does size matter? Study of performance of pseudo-ELISAs based on molecularly imprinted polymer nanoparticles prepared for analytes of different sizes
The aim of this work is to evaluate whether the size of the analyte used as template for the synthesis of molecularly imprinted polymer nanoparticles (nanoMIPs) can affect their performance in pseudo-enzyme linked immunosorbent assays (pseudo-ELISAs). Successful demonstration of a nanoMIPs-based pseudo-ELISA for vancomycin (1449.3 g mol) was demonstrated earlier. In the present investigation, the following analytes were selected: horseradish peroxidase (HRP, 44 kDa), cytochrome C (Cyt C, 12 kDa) biotin (244.31 g mol) and melamine (126.12 g mol). NanoMIPs with a similar composition for all analytes were synthesised by persulfate-initiated polymerisation in water. In addition, core-shell nanoMIPs coated with polyethylene glycol (PEG) and imprinted for melamine were produced in organics and tested. The polymerisation of the nanoparticles was done using a solid-phase approach with the correspondent template immobilised on glass beads. The performance of the nanoMIPs used as replacement for antibodies in direct pseudo-ELISA (for the enzymes) and competitive pseudo-ELISA for the smaller analytes was investigated. For the competitive mode we rely on competition for the binding to the nanoparticles between free analyte and corresponding analyte-HRP conjugate. The results revealed that the best performances were obtained for nanoMIPs synthesised in aqueous media for the larger analytes. In addition, this approach was successful for biotin but completely failed for the smallest template melamine. This problem was solved using nanoMIP prepared by UV polymerisation in an organic media with a PEG shell. This study demonstrates that the preparation of nanoMIP by solid-phase approach can produce material with high affinity and potential to replace antibodies in ELISA tests for both large and small analytes. This makes this technology versatile and applicable to practically any target analyte and diagnostic field
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