Single Assay for Simultaneous Detection and Differential Identification of Human and Avian Influenza Virus Types, Subtypes, and Emergent Variants

For more than four decades the cause of most type A influenza virus infections of humans has been attributed to only two viral subtypes, A/H1N1 or A/H3N2. In contrast, avian and other vertebrate species are a reservoir of type A influenza virus genome diversity, hosting strains representing at least 120 of 144 combinations of 16 viral hemagglutinin and 9 viral neuraminidase subtypes. Viral genome segment reassortments and mutations emerging within this reservoir may spawn new influenza virus strains as imminent epidemic or pandemic threats to human health and poultry production. Traditional methods to detect and differentiate influenza virus subtypes are either time-consuming and labor-intensive (culture-based) or remarkably insensitive (antibody-based). Molecular diagnostic assays based upon reverse transcriptase-polymerase chain reaction (RT-PCR) have short assay cycle time, and high analytical sensitivity and specificity. However, none of these diagnostic tests determine viral gene nucleotide sequences to distinguish strains and variants of a detected pathogen from one specimen to the next. Decision-quality, strain- and variant-specific pathogen gene sequence information may be critical for public health, infection control, surveillance, epidemiology, or medical/veterinary treatment planning. The Resequencing Pathogen Microarray (RPM-Flu) is a robust, highly multiplexed and target gene sequencing-based alternative to both traditional culture- or biomarker-based diagnostic tests. RPM-Flu is a single, simultaneous differential diagnostic assay for all subtype combinations of type A influenza viruses and for 30 other viral and bacterial pathogens that may cause influenza-like illness. These other pathogen targets of RPM-Flu may co-infect and compound the morbidity and/or mortality of patients with influenza. The informative specificity of a single RPM-Flu test represents specimen-specific viral gene sequences as determinants of virus type, A/HN subtype, virulence, host-range, and resistance to antiviral agents

Using Forums and Message Boards to Recruit Study Participants in Qualitative Research

The following observations have emerged from the author’s research experience involving the recruitment of focus group participants for a qualitative study on job search behaviors using technology. It is argued that Internet forums and message boards provide researchers with a robust approach to recruiting participants for qualitative study purposes. Advantages and characteristics of online communities are outlined to inform future practices based on the experience of engaging job-seekers who frequent a career advice board on the Internet. Challenges and limitations inherent to this methodology are also explored with techniques offered for maximizing the effectiveness of future recruitment. Use of Internet forums and message boards as objects of research versus as research tools is distinguished throughout. The paper contributes to a growing body of knowledge about harnessing the Internet to inform and support qualitative research.</jats:p

Interview with Margie Helm (MSS 552)

Oral history interview conducted on November 18, 1977 with Margie Helm, former Director of Western Kentucky University Libraries, about the beginnings of the Bowling Green-Warren County Library. A transcript of the interview is available under Additional Files