Additional file 1: Table S1. of Quantification by real-time PCR of Trypanosoma cruzi DNA in samples of Triatoma infestans used in xenodiagnosis of chronic Chagas disease patients

Xenodiagnosis (XD), PCR-xenodiagnosis (PCR-XD), real-time PCR-xenodiagnosis (qPCR-XD) and real-time PCR-blood (qPCR-B) results in 100 individuals with CChD. The ranged parasite burden are represented by numbers 0–5: 0 (No Ct); 1 (0.1–1 par. eq./ml); 2 (1–10 par. eq./ml); 3 (10–100 par. eq./ml); 4 (100–1,000 par. eq./ml) and 5 (1,000–10,000 par. eq./ml). (DOCX 22 kb

Assessing the risk zones of Chagas' disease in Chile, in a world marked by global climatic change

<div><p> BACKGROUND Vector transmission of Trypanosoma cruzi appears to be interrupted in Chile; however, data show increasing incidence of Chagas' disease, raising concerns that there may be a reemerging problem. OBJECTIVE To estimate the actual risk in a changing world it is necessary to consider the historical vector distribution and correlate this distribution with the presence of cases and climate change. METHODS Potential distribution models of Triatoma infestans and Chagas disease were performed using Maxent, a machine-learning method. FINDINGS Climate change appears to play a major role in the reemergence of Chagas' disease and T. infestans in Chile. The distribution of both T. infestans and Chagas' disease correlated with maximum temperature, and the precipitation during the driest month. The overlap of Chagas' disease and T. infestans distribution areas was high. The distribution of T. infestans, under two global change scenarios, showed a minimal reduction tendency in suitable areas. MAIN CONCLUSION The impact of temperature and precipitation on the distribution of T. infestans, as shown by the models, indicates the need for aggressive control efforts; the current control measures, including T. infestans control campaigns, should be maintained with the same intensity as they have at present, avoiding sylvatic foci, intrusions, and recolonisation of human dwellings.</p></div

Sensitivities and specificities of the <i>T. cruzi</i> satDNA and kDNA OligoC-TesTs on 187 Chagas patients and 114 endemic and non-endemic controls from Chile, Argentina, Spain, Sudan and D.R. Congo.

<p>Notes: 95% CI = 95% confidence interval; Non-end. = Non-endemic; I = first repetition; II = second repetition; N.D. = not done due to limited number of test kits available;</p><p>^a All Chagas patients recruited at the Hospital Ramón y Cajal in Madrid were from Bolivian origin.</p

Demographic characteristics of the participants recruited in the study and specifications of blood collections and reference tests.

<p>Notes: ND: not done; a: ELISA Chagas III, GrupoBios S.A., Chile; b: In house protocol with <i>T. cruzi</i> Tulahuen strain; c: Chagatest ELISA recombinant v. 4.0, Wiener Lab, Argentina; d: Chagatest HAI, Wiener Lab, Argentina; e: In house protocol with <i>T. cruzi</i> Dm28, MC & T strain; f: In house protocol with <i>T. cruzi</i> Dm28,MC & T strain.</p

Analytical sensitivity and analytical specificity of the <i>T. cruzi</i> satDNA OligoC-TesT and kDNA OligoC-TesT on DNA from 6 <i>T. cruzi</i> and 3 <i>T. rangeli</i> reference strains.

<p>DTU = discrete typing unit, NA = not applicable.</p