232 research outputs found

    Discussion and design of high vocational education

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    To set up a vocational technical college within a university is in accordance with the tendency of international high education. It is an important and practical move to develop high vocational education based on China\u27s realities. Although there is much controversy on decision-making and difficulties in operation, in a sense and in a particular period of time, promoting the all-round development of vocational education can not be underestimated. To greatly and timely promote this cause is one of the Key points in the developments of China\u27s 21st century vocational education

    New Insights into Protein Hydroxylation and Its Important Role in Human Diseases

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    Protein hydroxylation is a post-translational modification catalyzed by 2-oxoglutarate-dependent dioxygenases. The hydroxylation modification can take place on various amino acids, including but not limited to proline, lysine, asparagine, aspartate and histidine. A classical example of this modification is hypoxia inducible factor alpha (HIF-α) prolyl hydroxylation, which affects HIF-α protein stability via the Von-Hippel Lindau (VHL) tumor suppressor pathway, a Cullin 2-based E3 ligase adaptor protein frequently mutated in kidney cancer. In addition to protein stability regulation, protein hydroxylation may influence other post-translational modifications or the kinase activity of the modified protein (such as Akt and DYRK1A/B). In other cases, protein hydroxylation may alter protein-protein interaction and its downstream signaling events in vivo (such as OTUB1, MAPK6 and eEF2K). In this review, we highlight the recently identified protein hydroxylation targets and their pathophysiological roles, especially in cancer settings. Better understanding of protein hydroxylation will help identify novel therapeutic targets and their regulation mechanisms to foster development of more effective treatment strategies for various human cancers

    Coating titania nanoparticles with epoxy-containing catechol polymers via Cu(0)-living radical polymerization as intelligent enzyme carriers

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    Immobilization of enzyme could offer the biocatalyst with increased stability and important recoverability, which plays a vital role in the enzyme’s industrial applications. In this study, we present a new strategy to build an intelligent enzyme carrier by coating titania nanoparticles with thermoresponsive epoxy-functionalized polymers. Zero-valent copper-mediated living radical polymerization (Cu(0)-LRP) was utilized herein to copolymerize N-isopropylacrylamide (NIPAM) and glycidyl acrylate (GA) directly from an unprotected dopamine-functionalized initiator to obtain an epoxy-containing polymer with terminal anchor for the “grafting to” or “one-pot” modification of titania nanoparticles. A rhodamine B-labeled laccase has been subsequently used as a model enzyme for successful immobilization to yield an intelligent titania/laccase hybrid bifunctional catalyst. The immobilized laccase has shown excellent thermal stability under ambient or even relatively high temperature above the lower critical solution temperature (LCST) at which temperature the hybrid particles could be facilely recovered for reuse. The enzyme activity could be maintained during the repeated use after recovery and enzymatic degradation of bisphenol A was proven to be efficient. The photocatalytic ability of titania was also investigated by fast degradation of rhodamine B under the excitation of simulated sunlight. Therefore, this study has provided a facile strategy for the immobilization of metal oxide catalysts with enzymes, which constructs a novel bifunctional catalyst that will be promising for the “one-pot” degradation of different organic pollutants

    RAI-Net: Range-Adaptive LiDAR Point Cloud Frame Interpolation Network

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    LiDAR point cloud frame interpolation, which synthesizes the intermediate frame between the captured frames, has emerged as an important issue for many applications. Especially for reducing the amounts of point cloud transmission, it is by predicting the intermediate frame based on the reference frames to upsample data to high frame rate ones. However, due to high-dimensional and sparse characteristics of point clouds, it is more difficult to predict the intermediate frame for LiDAR point clouds than videos. In this paper, we propose a novel LiDAR point cloud frame interpolation method, which exploits range images (RIs) as an intermediate representation with CNNs to conduct the frame interpolation process. Considering the inherited characteristics of RIs differ from that of color images, we introduce spatially adaptive convolutions to extract range features adaptively, while a high-efficient flow estimation method is presented to generate optical flows. The proposed model then warps the input frames and range features, based on the optical flows to synthesize the interpolated frame. Extensive experiments on the KITTI dataset have clearly demonstrated that our method consistently achieves superior frame interpolation results with better perceptual quality to that of using state-of-the-art video frame interpolation methods. The proposed method could be integrated into any LiDAR point cloud compression systems for inter prediction.Comment: Accepted by the IEEE International Symposium on Broadband Multimedia Systems and Broadcasting 202

    Genome Editing of Pik3cd Impedes Abnormal Retinal Angiogenesis

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    Abnormal angiogenesis is associated with myriad human diseases including proliferative diabetic retinopathy. Signaling transduction via phosphoinositide 3-kinases (PI3Ks) plays a critical role in angiogenesis. Herein, we showed that p110δ, the catalytic subunit of PI3Kδ, was highly expressed in pathological retinal vascular endothelial cells (ECs) in a mouse model of oxygen-induced retinopathy (OIR) and in fibrovascular membranes from patients with proliferative diabetic retinopathy. To explore novel intervention with PI3Kδ expression, we developed a recombinant dual adeno-associated viral (rAAV) system for delivering CRISPR/Cas9 in which Streptococcus pyogenes (Sp) Cas9 expression was driven by an endothelial specific promoter of intercellular adhesion molecule 2 (pICAM2) to edit genomic Pik3cd, the gene encoding p110δ. We then demonstrated that infection of cultured mouse vascular endothelial cells with the dual rAAV1s of rAAV1-pICAM2-SpCas9 and rAAV1-SpGuide targeting genomic Pik3cd resulted in 80% DNA insertion/deletion in the locus of genomic Pik3cd and 70% depletion of p110δ expression. Furthermore, we showed that in the mouse model of OIR editing retinal Pik3cd with the dual rAAV1s resulted in not only a significant decrease in p110δ expression, and Akt activation, but also a dramatic reduction in pathological retinal angiogenesis. These findings reveal that Pik3cd editing is a novel approach to treating abnormal retinal angiogenesis
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