Elastic scattering of spin polarised electrons from krypton

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Spinresolved collisions of electrons with rubidium atoms: a search for relativistic effects

The search for relativistic effects in electron-alkali scattering is currently a topic of considerable interest. The A2 spin asymmetry parameter is a direct measure of relativistic effects in the electron-atom collision process, as it is entirely dependent on the spin-orbit effect. We present measurements of the A2 spin asymmetry for the 5S 5P transition in rubidium at incident energies of 15, 20, 30 and 50 eV and for elastic scattering at 15, 20, 30, 50 and 80eV. Our results indicate that under these collision conditions, relativistic effects are measurable, in qualitative agreement with the available theory

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Asymmetry of polarized electrons scattered elastically from krypton

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