Additional file 3: of The impact of reimbursement systems on equity in access and quality of primary care: A systematic literature review

Summary of the 22 studies in included in the systematic review. (DOCX 19 kb

Additional file 1: Figure S1-S16. of Cellular function reinstitution of offspring red blood cells cloned from the sickle cell disease patient blood post CRISPR genome editing

Function reinstitution of offspring red blood cells cloned from the sickle cell disease patient blood by a clinically practicable CRISPR/Cas9 method. (DOCX 4365 kb

Additional file 2: of Transcriptome display during tilapia sex determination and differentiation as revealed by RNA-Seq analysis

Figure S1. Cellular localization of C15orf65 and Rbp2 in tilapia testis and ovary by ISH. C15orf65 was highly expressed in primary spermatocytes and secondary spermatocytes (A), as well as in the oocytes (B). Rbp2 was expressed in spermatogonia and efferent duct (C), and in the phase II oocytes of the ovary (D). PSC, primary spermatocytes; SSC, secondary spermatocytes; OC, oocytes; I-IV, phase I to phase IV oocytes; SG, spermatogonia; ED, efferent duct; Arrowheads indicate the positive signal. (TIF 40771 kb

The children with visual handicap and pre-school education

The children with visual handicap are the same as the healthy children. They have the same basic physical, psychical and feeling needs. Yet in addition, they have got special educational needs caused by their handicap. If they grow up in a good, stimulating and friendly surrounding, with suitable education program and global tyflopedic care, a lot of them can successfully join other adults in the society. We perceive our surrounding with all senses. The sense analyzers are working in inseparable mutuality. We can touch if vision is impossible. The sense of touch is spread all over our body, so we are feeling something continuously. We are attracted or repulsed by different materials. The sense of hearing is interpreting information about our surroundings. We can close our eyes, but hearing perceives all the time. Also the sense of smelling transfers messages from places wee cannot see. Well known smell can cause us to remember some images from our memory. Taste aids our imagination in the same way. Correctly performed sense exercises are the key issue in the integration of the children with visual handicap info the human society. The basic idea of the sense exercises is training the healthy senses to be able to compensate and reeducate missing or damaged vision

Additional file 1: of Transcriptome display during tilapia sex determination and differentiation as revealed by RNA-Seq analysis

Candidate genes involved in tilapia sex determination and differentiation. (XLSX 65 kb

Characterization of Gonadal Transcriptomes from Nile Tilapia (<i>Oreochromis niloticus</i>) Reveals Differentially Expressed Genes

<div><p>Four pairs of XX and XY gonads from Nile tilapia were sequenced at four developmental stages, 5, 30, 90, and 180 days after hatching (dah) using Illumina Hiseq^TM technology. This produced 28 Gb sequences, which were mapped to 21,334 genes. Of these, 259 genes were found to be specifically expressed in XY gonads, and 69 were found to be specific to XX gonads. Totally, 187 XX- and 1,358 XY-enhanced genes were identified, and 2,978 genes were found to be co-expressed in XX and XY gonads. Almost all steroidogenic enzymes, including <i>cyp19a1a</i>, were up-regulated in XX gonads at 5 dah; but in XY gonads these enzymes, including <i>cyp11b2</i>, were significantly up-regulated at 90 dah, indicating that, at a time critical to sex determination, the XX fish produced estrogen and the XY fish did not produce androgens. The most pronounced expression of steroidogenic enzyme genes was observed at 30 and 90 dah for XX and XY gonads, corresponding to the initiation of germ cell meiosis in the female and male gonads, respectively. Both estrogen and androgen receptors were found to be expressed in XX gonads, but only estrogen receptors were expressed in XY gonads at 5 dah. This could explain why exogenous steroid treatment induced XX and XY sex reversal. The XX-enhanced expression of <i>cyp19a1a</i> and <i>cyp19a1b</i> at all stages suggests an important role for estrogen in female sex determination and maintenance of phenotypic sex. This work is the largest collection of gonadal transcriptome data in tilapia and lays the foundation for future studies into the molecular mechanisms of sex determination and maintenance of phenotypic sex in non-model teleosts.</p></div

Pipeline of experiment (A) and bioinformatics analysis (B).

<p>Pipeline of experiment (A) and bioinformatics analysis (B).</p

Five types of genes in XX and XY transcriptomes of tilapia gonads.

<p>A hierarchical clustering of differentially expressed transcripts (FDR≤10⁻² and |log₂ (XX_RPKM/XY_RPKM)|≥1) generated five types of genes. “SEGs” indicate genes specific to either XX or XY. “ND-SEGs” indicate SEGs that did not meet statistical criteria. “XX-DIGs” indicate XX differentially expressed genes. “XY-DIGs” indicate XY differentially expressed genes. “COGs” indicate XX and XY co-expressed genes.</p

The expression profile of 17 genes* coding for steroidogenic enzymes.

<p>Note: * For the complete list of genes see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063604#pone-0063604-g005" target="_blank">Figure 5</a>. ** “Sum” indicates the total RPKM of the 17 steroidogenic enzymes; “average” indicates the average RPKM of the 17 steroidogenic enzymes; “mostdiff” indicates the most dramatically differentially expressed gene among the 17 steroidogenic enzymes at each stage.</p

Read counts, gene counts, and RPKM values of developing tilapia XX/XY gonads.

<p>Read counts, gene counts, and RPKM values of developing tilapia XX/XY gonads.</p