Allozyme Evidence for the Origin and Diversification of Gossypium barbadense L.

Gossypium barbadense L. is a commercially important cotton species of tropical South American origin presently grownin many regions of the world. The species is morphologically diverse, consisting of a wide range of wild (or feral), commensal, landrace, and highly improvedcommercial forms. We performed allozyme analysis on 153 accessions representing the spectrum of G. barbadense diversityto ascertain the geographic origin of the species, its patterns of diffusion subsequent to domestication, and to reveal infraspecific relationships. Levels ofgenetic variation in G. barbadense are moderate. Of 59 loci scored, 24 were polymorphic, with a mean number of alleles perlocus of 1.69 and an average panmictic heterozygosity of 0.062. Principal component analysis revealed geographic clustering of accessions into six relativelydiscrete regions. Gene frequencies at many loci are significantly heterogeneous among these regions, with an average G STof 0.272. Northwestern South America contains the greatest genetic variability; we suggest that this region is the ancestral home of the species. The data indicate separate diffusion pathways from this region into Argentina-Paraguay and into eastern and northern South America east of the Andes. Caribbean Island and Central American forms appear to be derived from the latter. These diffusion pathways are in accordance with morphological evidence and historical record. In contrast to expectations based on geographic proximity, Pacific Island forms have their closest affinity to accessions from eastern South America. Advanced cultivated stocks seem largely derived from western Andean material, but also contain introgressed G. hirsutum germ plasm. Introgression was relatively high (22%-50% of accessions) in commercial stocks and in forms from Argentina-Paraguay and various Pacific Islands, but was conspicuously low or absent in material from Central America and the Caribbean, where commensal and commercial forms of both species are sympatric

Evolving concepts of lunar architecture: The potential of subselene development

In view of the superior environmental and operational conditions that are thought to exist in lava tubes, popular visions of permanent settlements built upon the lunar surface may prove to be entirely romantic. The factors that will ultimately come together to determine the design of a lunar base are complex and interrelated, and they call for a radical architectural solution. Whether lunar surface-deployed superstructures can answer these issues is called into question. One particularly troublesome concern in any lunar base design is the need for vast amounts of space, and the ability of man-made structures to provide such volumes in a reliable pressurized habitat is doubtful. An examination of several key environmental design issues suggests that the alternative mode of subselene development may offer the best opportunity for an enduring and humane settlement

Wind-tunnel free-flight investigation of a supersonic persistence fighter

Wind-tunnel free-flight tests have been conducted in the Langley 30- by 60-Foot Wind Tunnel to examine the high-angle-of-attack stability and control characteristics and control law design of a supersonic persistence fighter (SSPF) at 1 g flight conditions. In addition to conventional control surfaces, the SSPF incorporated deflectable wingtips (tiperons) and pitch and yaw thrust vectoring. A direct eigenstructure assignment technique was used to design control laws to provide good flying characteristics well into the poststall angle-of-attack region. Free-flight tests indicated that it was possible to blend effectively conventional and unconventional control surfaces to achieve good flying characteristics well into the poststall angle-of-attack region

Quenched and Negative Hall Effect in Periodic Media: Application to Antidot Superlattices

We find the counterintuitive result that electrons move in OPPOSITE direction to the free electron E x B - drift when subject to a two-dimensional periodic potential. We show that this phenomenon arises from chaotic channeling trajectories and by a subtle mechanism leads to a NEGATIVE value of the Hall resistivity for small magnetic fields. The effect is present also in experimentally recorded Hall curves in antidot arrays on semiconductor heterojunctions but so far has remained unexplained.Comment: 10 pages, 4 figs on request, RevTeX3.0, Europhysics Letters, in pres

β ‐ sheet assembly in amyloidogenic glutamic acid nanostructures: insights from X‐ray scattering and infrared nanospectroscopy

Glutamic acid–rich peptides are crucial to a variety of biological processes, including glutamatergic neurotransmission and immunological defense. Glutamic acid sequences often exhibit unusual organization into β2‐type sheets, where bifurcated H bonds formed between glutamic acid side chains and NH in amide bonds on adjacent β‐strands play a paramount role for stabilizing the molecular assembly. Herein, we investigate the self‐assembly and supramolecular structure of simplified models consisting of alternating glutamic acid/phenylalanine residues. Small‐angle X‐ray scattering and atomic force microscopy show that the aggregation pathway is characterized by the formation of small oligomers, followed by coalescence into nanofibrils and nanotapes. Amyloidogenic features are further demonstrated through fiber X‐ray diffraction, which reveal molecular packing according to cross‐β patterns, where β‐strands appear perpendicularly oriented to the long axis of nanofibrils and nanotapes. Nanoscale infrared spectroscopy from individual nanoparticles on dried samples shows a remarkable decrease of β2‐sheet content, accompanied by growth of standard β‐sheet fractions, indicating a β2‐to‐β1 transition as a consequence of the release of solvent from the interstices of peptide assemblies. Our findings highlight the key role played by water molecules in mediating H‐bond formation in β2‐sheets commonly found in amyloidogenic glutamic acid–rich aggregates