88 research outputs found
Serologic Survey of Hantavirus Infection, Brazilian Amazon
Federal University of MaranhĂŁo. SĂŁo LuĂs, MA, Brazil.Federal University of MaranhĂŁo. SĂŁo LuĂs, MA, Brazil.Federal University of MaranhĂŁo. SĂŁo LuĂs, MA, Brazil.Federal University of MaranhĂŁo. SĂŁo LuĂs, MA, Brazil.Federal University of MaranhĂŁo. SĂŁo LuĂs, MA, Brazil.Federal University of MaranhĂŁo. SĂŁo LuĂs, MA, Brazil.State Department of Health. SĂŁo LuĂs, MA, Brazil.MinistĂ©rio da SaĂșde. Secretaria de VigilĂąncia em SaĂșde. Instituto Evandro Chagas. BelĂ©m, PA, Brasil.MinistĂ©rio da SaĂșde. Secretaria de VigilĂąncia em SaĂșde. Instituto Evandro Chagas. BelĂ©m, PA, Brasil
L-NAME treatment enhances exercise-induced content of myocardial heat shock protein 72 (Hsp72) in rats
Background/Aim: Nitric oxide (NO) modulates the expression of the chaperone Hsp72 in the heart, and exercise stimulates both NO production and myocardial Hsp72 expression. The main purpose of the study was to investigate whether NO interferes with an exercise-induced myocardial Hsp72 expression. Methods: Male Wistar rats (70-100 days) were divided into control (C, n= 12), L-NAME-treated (L, n= 12), exercise (E, n= 13) and exercise plus L-NAME-treated (EL, n= 20) groups. L-NAME was given in drinking water (700 mg. L-1) and the exercise was performed on a treadmill (15-25 m.min(-1), 40-60 min. day(-1)) for seven days. Left ventricle (LV) protein Hsp content, NOS and phosphorylated-NOS (p-NOS) isoforms were measured using Western blotting. The activity of NOS was assayed in LV homogenates by the conversion of [H-3] L-arginine to [H-3] L-citrulline. Results: Hsp72 content was increased significantly (223%; p < 0.05) in the E group compared to the C group, but exercise alone did not alter the NOS content, p-NOS isoforms or NOS activity. Contrary to our expectation, L-NAME enhanced (p < 0.05) the exercise-induced Hsp72 content (EL vs. C, L and E groups = 1019%, 548% and 457%, respectively). Although the EL group had increased stimulatory p-eNOS(Ser1177) (over 200%) and decreased inhibitory p-nNOS(Ser852) (similar to 50%) compared to both the E and L groups (p < 0.05), NOS activity was similar in all groups. Conclusions: Our results suggest that exercise-induced cardiac Hsp72 expression does not depend on NO. Conversely, the in vivo L-NAME treatment enhances exercise-induced Hsp72 production. This effect may be due to an increase in cardiac stress. Copyright (C) 2011 S. Karger AG, Basel275479486CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTĂFICO E TECNOLĂGICO - CNPQFUNDAĂĂO DE AMPARO Ă PESQUISA E INOVAĂĂO DO ESPĂRITO SANTO - FAPESsem informaçã
Novel insights into host-fungal pathogen interactions derived from live-cell imaging
Acknowledgments The authors acknowledge funding from the Wellcome Trust (080088, 086827, 075470 and 099215) including a Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology 097377 and FP7-2007â2013 grant agreement HEALTH-F2-2010-260338âALLFUN to NARG.Peer reviewedPublisher PD
Desigualdades sociais e uso de serviços de saĂșde: evidĂȘncias de anĂĄlise estratificada
Gas exchange in massai grass under five nitrogen fertilization levels during establishment and regrowth
Respostas ecofisiolĂłgicas de cafeeiros submetidos ao deficit hĂdrico para concentração da florada no Cerrado de Minas Gerais
Compatibility of vegetable fibers with Portland cement and its relationship with the physical properties
Diversidade genĂ©tica estimada com marcadores entre sequĂȘncias simples repetidas em cultivos comerciais de Cupuaçuzeiro
CaracterĂsticas da Carcaça e Qualidade da Carne de Tourinhos Alimentados com Dietas de Alta Energia
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