Real-time monitoring of calcium carbonate and cationic peptide deposition on carboxylate-SAM using a microfluidic SAW biosensor

A microfluidic biosensor with surface acoustic wave technology was used in this study to monitor the interaction of calcium carbonate with standard carboxylate self-assembled monolayer sensor chips. Different fluids, with and without biomolecular components, were investigated. The pH-dependent surface interactions of two bio-inspired cationic peptides, AS8 and ES9, which are similar to an extracellular domain of the chitin synthase involved in mollusc shell formation, were also investigated in a biological buffer system. A range of experimental conditions are described that are suitable to study non-covalent molecular interactions in the presence of ionic substances, such as, mineral precursors below the solubility equilibrium. The peptide ES9, equal to the mollusc chitin synthase epitope, is less sensitive to changes in pH than its counterpart AS8 with a penta-lysine core, which lacks the flanking acidic residues. This study demonstrates the extraordinary potential of microfluidic surface acoustic wave biosensors to significantly expand our experimental capabilities for studying the principles underlying biomineralization in vitro

War-time military service can affect partisan preferences

Does war-time military service affect partisan preferences? We argue that military service increases the salience and potential costs of war. Therefore, soldiers who serve during mismanaged wars will associate the ruling party with incompetence and be less likely to support the ruling party in the future. To test our argument, we analyze almost 50 years of Israel National Election Studies. Employing a regression discontinuity design, we show that compared with respondents who were too young to serve in the Yom Kippur war, respondents just old enough to serve report lower support for the Labor party well after the war ended. This effect is likely driven by soldiers’ unwillingness to support a party they associate with security incompetence. We further show that the negative effect of military service does not materialize in well-managed wars, contributing to the literature on the political consequences of war and attitude formation

Real-time monitoring of calcium carbonate and cationic peptide deposition on carboxylate-SAM using a microfluidic SAW biosensor

A microfluidic biosensor with surface acoustic wave technology was used in this study to monitor the interaction of calcium carbonate with standard carboxylate self-assembled monolayer sensor chips. Different fluids, with and without biomolecular components, were investigated. The pH-dependent surface interactions of two bio-inspired cationic peptides, AS8 and ES9, which are similar to an extracellular domain of the chitin synthase involved in mollusc shell formation, were also investigated in a biological buffer system. A range of experimental conditions are described that are suitable to study non-covalent molecular interactions in the presence of ionic substances, such as, mineral precursors below the solubility equilibrium. The peptide ES9, equal to the mollusc chitin synthase epitope, is less sensitive to changes in pH than its counterpart AS8 with a penta-lysine core, which lacks the flanking acidic residues. This study demonstrates the extraordinary potential of microfluidic surface acoustic wave biosensors to significantly expand our experimental capabilities for studying the principles underlying biomineralization in vitro

Prävalenz und Prädiktoren für Rauschtrinken : Querschnittsbefragung repräsentativ für Niedersachsen

Diese Artikelfassung entspricht nicht vollständig dem in der Zeitschrift Sucht veröffentlichten Artikel unter https://econtent.hogrefe.com/doi/10.1024/0939-5911/a000622. Dies ist nicht die Originalversion des Artikels und kann daher nicht zur Zitierung herangezogen werden. Bitte verbreiten oder zitieren Sie diesenArtikel nicht ohne Zustimmung des Autors.Zielsetzung: Ziel der Studie war es, die Prävalenz von Rauschtrinken in der ab 16-jährigen Bevölkerung Niedersachsens zu erfassen und Prädiktoren für dieses Risikoverhalten zu identifizieren. Methodik: Eine repräsentative Querschnittserhebung mit N = 5711 Teilnehmern ab 16 Jahren wurde schriftlich 2014 durch das Kriminologische Forschungsinstitut Niedersachsen (KFN) durchgeführt. Rauschtrinken wurde als mind. 5 Gläser Alkohol zu einer Gelegenheit/mind. 1x in den letzten 30 Tagen definiert. Neben deskriptiven Methoden wurde eine binär-logistische Regression mit Rauschtrinken als abhängige Variable durchgeführt. Ergebnisse: Die Prävalenz von Rauschtrinken lag bei 28 Prozent; signifikant abnehmend bei zunehmendem Alter. Männer, außerhalb von Großstädten lebende Personen und Teilnehmer ohne Migrationshintergrund waren signifikant häufiger involviert. Tabak-, Cannabiskonsum, Glücksspiel, Verträglichkeit, risikoreiche Verhaltensmuster, regelmäßige soziale Aktivitäten und soziale Integration wurden als Risikofaktoren identifiziert. Regelmäßige kulturelle Aktivität und islamische Religion waren negativ mit Rauschtrinken assoziiert. Schlussfolgerung: Neben soziodemographischen Prädiktoren wurden auch Faktoren wie Freizeitaktivitäten als Prädiktor identifiziert. Hier könnten niederschwellige präventive Maßnahmen im Sinne der Umgebungsgestaltung hinsichtlich ihrer Akzeptanz überprüft werden. Verhaltenspräventiv sollte der risikoerhöhende gleichzeitige Konsum anderer Substanzen fokussiert werden

Fermi-Dirac statistics and the number theory

We relate the Fermi-Dirac statistics of an ideal Fermi gas in a harmonic trap to partitions of given integers into distinct parts, studied in number theory. Using methods of quantum statistical physics we derive analytic expressions for cumulants of the probability distribution of the number of different partitions.Comment: 7pages, 2 figures, epl.cls, revised versio

Behavioral and Environmental Residential Risk Factors for Lyme Disease in the Southern Tier of New York

During the past 40 years, Lyme disease has emerged as the most widely reported vector-borne illness in the U.S., with the majority of cases occurring in the Northeastern United States. The pathogenic bacterium Borrelia burgdorferi relies on the deer tick, Ixodes scapularis, the primary enzootic vector responsible for the passage of the bacteria to humans. Behavioral risk factors for tick-borne diseases include those that increase the likelihood of being bit by a tick, including contact with vegetation, exposed skin, and spending time outdoors, all of which can occur within one’s residential yard. Risk factors within the assumed safety net of the home have not been extensively studied, nor has the effects of pet ownership, nor the presence of wild animals within the yard. During 2019-2020 we conducted 130 surveys of households in Broome and Chenango Counties, and performed tick drags in the rear yards of 102 of these homes. Risk factors included household members’ time spent in yard, type of outside activity, use of tick control methods, seasonal activity, and pet ownership, as well as specifics of the yard, including amount and type of vegetation and presence of wild animals. The household’s history of tick bites and infection were also recorded. A risk analysis is currently underway to determine which factors contribute to a higher risk of contracting tick-borne illnesses. This study is part of our ongoing effort to understand the threat of tick-borne diseases in peri-urban and urban settings within the Southern Tier of New York.https://orb.binghamton.edu/research_days_posters_2022/1034/thumbnail.jp

Structure-property relationships in mechanically stimulated Sorghum bicolor stalks

Mechanical properties of plants and underlying structure-property relationships are important for agricultural purposes as well as for biomimetic concepts. In this study, the effect of mechanical stimulation on morphology and bending properties of the stalk was investigated for Sorghum bicolor (Poaceae), a widely used drought-tolerant biomass grass. An experimental set-up allowing for defined growth and mechanical perturbation (flexing) during a defined growth period was designed. Mechanical properties of individual internodes of the stalk were determined by three-point bending tests. We found that the three investigated lines showed differences in their general bending strength in the non-stimulated condition. However, similar high range of bending strength values was measured for all plant lines after they underwent the mechanical stimulation procedure. The anatomy of internode cross-sections was examined to evaluate structure-property relationships. An increased thickness of the outer sclerenchymatous tissue was observed for internodes with higher bending strength values. Dried internodes fail under lower strains but showed higher bending strength. These findings show that mechanosensitivity in sorghum is dependent on genetic as well as environmental factors. The experimental system presented here offers new straight-forward possibilities for S. bicolor line selection for applications requiring mechanical strength of the stalk

Type I interferon signaling is required for activation of the inflammasome during Francisella infection

Francisella tularensis is a pathogenic bacterium whose virulence is linked to its ability to replicate within the host cell cytosol. Entry into the macrophage cytosol activates a host-protective multimolecular complex called the inflammasome to release the proinflammatory cytokines interleukin (IL)-1β and -18 and trigger caspase-1–dependent cell death. In this study, we show that cytosolic F. tularensis subspecies novicida (F. novicida) induces a type I interferon (IFN) response that is essential for caspase-1 activation, inflammasome-mediated cell death, and release of IL-1β and -18. Extensive type I IFN–dependent cell death resulting in macrophage depletion occurs in vivo during F. novicida infection. Type I IFN is also necessary for inflammasome activation in response to cytosolic Listeria monocytogenes but not vacuole-localized Salmonella enterica serovar Typhimurium or extracellular adenosine triphosphate. These results show the specific connection between type I IFN signaling and inflammasome activation, which are two sequential events triggered by the recognition of cytosolic bacteria. To our knowledge, this is the first example of the positive regulation of inflammasome activation. This connection underscores the importance of the cytosolic recognition of pathogens and highlights how multiple innate immunity pathways interact before commitment to critical host responses

The Infrared Imaging Spectrograph (IRIS) for TMT: Data Reduction System

IRIS (InfraRed Imaging Spectrograph) is the diffraction-limited first light instrument for the Thirty Meter Telescope (TMT) that consists of a near-infrared (0.84 to 2.4 $\mu$m) imager and integral field spectrograph (IFS). The IFS makes use of a lenslet array and slicer for spatial sampling, which will be able to operate in 100's of different modes, including a combination of four plate scales from 4 milliarcseconds (mas) to 50 mas with a large range of filters and gratings. The imager will have a field of view of 34$\times$34 arcsec$^{2}$ with a plate scale of 4 mas with many selectable filters. We present the preliminary design of the data reduction system (DRS) for IRIS that need to address all of these observing modes. Reduction of IRIS data will have unique challenges since it will provide real-time reduction and analysis of the imaging and spectroscopic data during observational sequences, as well as advanced post-processing algorithms. The DRS will support three basic modes of operation of IRIS; reducing data from the imager, the lenslet IFS, and slicer IFS. The DRS will be written in Python, making use of open-source astronomical packages available. In addition to real-time data reduction, the DRS will utilize real-time visualization tools, providing astronomers with up-to-date evaluation of the target acquisition and data quality. The quicklook suite will include visualization tools for 1D, 2D, and 3D raw and reduced images. We discuss the overall requirements of the DRS and visualization tools, as well as necessary calibration data to achieve optimal data quality in order to exploit science cases across all cosmic distance scales.Comment: 13 pages, 2 figures, 6 tables, Proceeding 9913-165 of the SPIE Astronomical Telescopes + Instrumentation 201