A pharmacological cocktail for arresting actin dynamics in living cells.

The actin cytoskeleton is regulated by factors that influence polymer assembly, disassembly, and network rearrangement. Drugs that inhibit these events have been used to test the role of actin dynamics in a wide range of cellular processes. Previous methods of arresting actin rearrangements take minutes to act and work well in some contexts, but can lead to significant actin reorganization in cells with rapid actin dynamics, such as neutrophils. In this paper, we report a pharmacological cocktail that not only arrests actin dynamics but also preserves the structure of the existing actin network in neutrophil-like HL-60 cells, human fibrosarcoma HT1080 cells, and mouse NIH 3T3 fibroblast cells. Our cocktail induces an arrest of actin dynamics that initiates within seconds and persists for longer than 10 min, during which time cells maintain their responsivity to external stimuli. With this cocktail, we demonstrate that actin dynamics, and not simply morphological polarity or actin accumulation at the leading edge, are required for the spatial persistence of Rac activation in HL-60 cells. Our drug combination preserves the structure of the existing cytoskeleton while blocking actin assembly, disassembly, and rearrangement, and should prove useful for investigating the role of actin dynamics in a wide range of cellular signaling contexts

Enhancement and evaluation of Skylab photography for potential land use inventories, part 1

The author has identified the following significant results. Three sites were evaluated for land use inventory: Finger Lakes - Tompkins County, Lower Hudson Valley - Newburgh, and Suffolk County - Long Island. Special photo enhancement processes were developed to standardize the density range and contrast among S190A negatives. Enhanced black and white enlargements were converted to color by contact printing onto diazo film. A color prediction model related the density values on each spectral band for each category of land use to the spectral properties of the various diazo dyes. The S190A multispectral system proved to be almost as effective as the S190B high resolution camera for inventorying land use. Aggregate error for Level 1 averaged about 12% while Level 2 aggregate error averaged about 25%. The S190A system proved to be much superior to LANDSAT in inventorying land use, primarily because of increased resolution

Transform-limited pulses are not optimal for resonant multiphoton transitions

Maximizing nonlinear light-matter interactions is a primary motive for compressing laser pulses to achieve ultrashort transform limited pulses. Here we show how, by appropriately shaping the pulses, resonant multiphoton transitions can be enhanced significantly beyond the level achieved by maximizing the pulse's peak intensity. We demonstrate the counterintuitive nature of this effect with an experiment in a resonant two-photon absorption, in which, by selectively removing certain spectral bands, the peak intensity of the pulse is reduced by a factor of 40, yet the absorption rate is doubled. Furthermore, by suitably designing the spectral phase of the pulse, we increase the absorption rate by a factor of 7.Comment: 4 pages, 3 figure

Sympathetic cooling and collisional properties of a Rb-Cs mixture

We report on measurements of the collisional properties of a mixture of $^{133}$Cs and $^{87}$Rb atoms in a magnetic trap at $\mu\mathrm{K}$ temperatures. By selectively evaporating the Rb atoms using a radio-frequency field, we achieved sympathetic cooling of Cs down to a few $\mu\mathrm{K}$. The inter-species collisional cross-section was determined through rethermalization measurements, leading to an estimate of $a_s=595 a_0$ for the s-wave scattering length for Rb in the $|F=2, m_F=2>$ and Cs in the $|F=4, m_F=4>$ magnetic states. We briefly speculate on the prospects for reaching Bose-Einstein condensation of Cs inside a magnetic trap through sympathetic cooling

Gauge/Anomaly Syzygy and Generalized Brane World Models of Supersymmetry Breaking

In theories in which SUSY is broken on a brane separated from the MSSM matter fields, supersymmetry breaking is naturally mediated in a variety of ways. Absent other light fields in the theory, gravity will mediate supersymmetry breaking through the conformal anomaly. If gauge fields propagate in the extra dimension they, too, can mediate supersymmetry breaking effects. The presence of gauge fields in the bulk motivates us to consider the effects of new messenger fields with holomorphic and non-holomorphic couplings to the supersymmetry breaking sector. These can lead to contributions to the soft masses of MSSM fields which dramatically alter the features of brane world scenarios of supersymmetry breaking. In particular, they can solve the negative slepton mass squared problem of anomaly mediation and change the predictions of gaugino mediation.Comment: 4 pages, RevTe

Collective modes and correlations in one-component plasmas

The static and time-dependent potential and surface charge correlations in a plasma with a boundary are computed for different shapes of the boundary. The case of a spheroidal or spherical one-component plasma is studied in detail because experimental results are available for such systems. Also, since there is some knowlegde both experimental and theoretical about the electrostatic collective modes of these plasmas, the time-dependent correlations are computed using a method involving these modes.Comment: 20 pages, plain TeX, submitted to Phys. Rev.

High-Power Broadly Tunable Electrooptic Frequency Comb Generator

Broadband traveling-wave electrooptic modulators made of lithium niobate have reached a high level of technological maturity. They can provide simultaneously low V pi, sustain high power (both optical and RF) and yet provide low propagation loss. By combining together these features, we present a high-power handling, broadly tunable, electrooptic frequency comb generator. The device produces between 60 and 75 lines within -10 dB bandwidth over its full tuning range-from 6 to 18 GHz- and can handle up to 1 W of optical input power. This optical frequency comb platform is very well suited for applications in RF photonics and optical communications that require independent RF and optical tuning as well as high-repetition rates but moderate bandwidth

Relative uptake of minoxidil into appendages and stratum corneum and permeation through human skin in vitro

We examined uptake of the model therapeutic agent, minoxidil, into appendages, stratum corneum (SC), and through human skin, under the influence of different vehicles. Quantitative estimation of therapeutic drug deposition into all three areas has not previously been reported. Finite doses of minoxidil (2%, w/v) in formulations containing varying amounts of ethanol, propylene glycol (PG), and water (60:20:20, 80:20:0, and 0:80:20 by volume, respectively) were used. Minoxidil in SC (by tape stripping), appendages (by cyanoacrylate casting), and receptor fluid was determined by liquid scintillation counting. At early times (30 min, 2 h), ethanol-containing formulations (60:20:20 and 80:20:0) caused significantly greater minoxidil retention in SC and appendages, compared to the formulation lacking ethanol (0:80:20). A significant increase in minoxidil receptor penetration occurred with the PG-rich 0:80:20 formulation after 12 h. We showed that deposition of minoxidil into appendages, SC, and skin penetration into receptor fluid were similar in magnitude. Transport by the appendageal route is likely to be a key determinant of hair growth promotion by minoxidil. © 2009 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 99:712–718, 2010Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/64562/1/21856_ftp.pd

Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo.

We demonstrate the utility of the phytochrome system to rapidly and reversibly recruit proteins to specific subcellular regions within specific cells in a living vertebrate embryo. Light-induced heterodimerization using the phytochrome system has previously been used as a powerful tool to dissect signaling pathways for single cells in culture but has not previously been used to reversibly manipulate the precise subcellular location of proteins in multicellular organisms. Here we report the experimental conditions necessary to use this system to manipulate proteins in vivo. As proof of principle, we demonstrate that we can manipulate the localization of the apical polarity protein Pard3 with high temporal and spatial precision in both the neural tube and the embryo's enveloping layer epithelium. Our optimizations of optogenetic component expression and chromophore purification and delivery should significantly lower the barrier for establishing this powerful optogenetic system in other multicellular organisms