51 research outputs found
Menselijke urine als meststof voor eetbare planten : Inzameling, medicijnresten, wet- en regelgeving en maatschappelijke acceptatie
Sulfur reduction in acid rock drainage environments
Microbiological suitability of acidophilic sulfur reduction for metal recovery was explored by enriching sulfur reducers from acidic sediments at low pH (from 2 to 5) with hydrogen, glycerol, methanol and acetate as electron donors at 30 °C. The highest levels of sulfide in the enrichments were detected at pH 3 with hydrogen and pH 4 with acetate. Cloning and sequencing of the 16S rRNA gene showed dominance of the deltaproteobacterial sulfur-reducing genus Desulfurella in all the enrichments and subsequently an acidophilic strain (TR1) was isolated. Strain TR1 grew at a broad range of pH (37) and temperature (2050 °C) and showed good metal tolerance (Pb2+, Zn2+, Cu2+, Ni2+), especially for Ni2+ and Pb2+, with maximal tolerated concentrations of 0.09 and 0.03 mM, respectively. Different sources of sulfur were tested in the enrichments, from which biosulfur showed fastest growth (doubling time of 1.9 days), followed by colloidal, chemical and sublimated sulfur (doubling times of 2.2, 2.5, and 3.6 days, respectively). Strain TR1?s physiological traits make it a good candidate to cope with low pH and high metal concentration in biotechnological processes for treatment of metal-laden acidic streams at low and moderately high temperature
Cometabolic Degradation of Trichloroethylene by Pseudomonas cepacia G4 in a Chemostat with Toluene as the Primary Substrate
Pseudomonas cepacia G4 is capable of cometabolic degradation of trichloroethylene (TCE) if the organism is grown on certain aromatic compounds. To obtain more insight into the kinetics of TCE degradation and the effect of TCE transformation products, we have investigated the simultaneous conversion of toluene and TCE in steady-state continuous culture. The organism was grown in a chemostat with toluene as the carbon and energy source at a range of volumetric TCE loading rates, up to 330 mumol/liter/h. The specific TCE degradation activity of the cells and the volumetric activity increased, but the efficiency of TCE conversion dropped when the TCE loading was elevated from 7 to 330 mumol/liter/h. At TCE loading rates of up to 145 mumol/liter/h, the specific toluene conversion rate and the molar growth yield of the cells were not affected by the presence of TCE. The response of the system to varying TCE loading rates was accurately described by a mathematical model based on Michaelis-Menten kinetics and competitive inhibition. A high load of 3,400 mumol of TCE per liter per h for 12 h caused inhibition of toluene and TCE conversion, but reduction of the TCE load to the original nontoxic level resulted in complete recovery of the system within 2 days. These results show that P. cepacia can stably and continuously degrade toluene and TCE simultaneously in a single-reactor system without biomass retention and that the organism is more resistant to high concentrations and shock loadings of TCE than Methylosinus trichosporium OB3b
Piezo-tolerant natural gas-producing microbes under accumulating pCO2
<p>Background: It is known that a part of natural gas is produced by biogenic degradation of organic matter, but the microbial pathways resulting in the formation of pressurized gas fields remain unknown. Autogeneration of biogas pressure of up to 20 bar has been shown to improve the quality of biogas to the level of biogenic natural gas as the fraction of CO2 decreased. Still, the pCO2 is higher compared to atmospheric digestion and this may affect the process in several ways. In this work, we investigated the effect of elevated pCO2 of up to 0.5 MPa on Gibbs free energy, microbial community composition and substrate utilization kinetics in autogenerative high-pressure digestion. Results: In this study, biogas pressure (up to 2.0 MPa) was batch-wise autogenerated for 268 days at 303 K in an 8-L bioreactor, resulting in a population dominated by archaeal Methanosaeta concilii, Methanobacterium formicicum and Mtb. beijingense and bacterial Kosmotoga-like (31% of total bacterial species), Propioniferax-like (25%) and Treponema-like (12%) species. Related microorganisms have also been detected in gas, oil and abandoned coal-bed reservoirs, where elevated pressure prevails. After 107 days autogeneration of biogas pressure up to 0.50 MPa of pCO2, propionate accumulated whilst CH4 formation declined. Alongside the Propioniferax-like organism, a putative propionate producer, increased in relative abundance in the period of propionate accumulation. Complementary experiments showed that specific propionate conversion rates decreased linearly from 30.3 mg g−1 VSadded day−1 by more than 90% to 2.2 mg g−1 VSadded day−1 after elevating pCO2 from 0.10 to 0.50 MPa. Neither thermodynamic limitations, especially due to elevated pH2, nor pH inhibition could sufficiently explain this phenomenon. The reduced propionate conversion could therefore be attributed to reversible CO2-toxicity. Conclusions: The results of this study suggest a generic role of the detected bacterial and archaeal species in biogenic methane formation at elevated pressure. The propionate conversion rate and subsequent methane production rate were inhibited by up to 90% by the accumulating pCO2 up to 0.5 MPa in the pressure reactor, which opens opportunities for steering carboxylate production using reversible CO2-toxicity in mixed-culture microbial electrosynthesis and fermentation.</p
A case in support of implementing innovative bio-processes in the metal mining industry
The metal mining industry faces many large challenges in future years, among which is the increasing need to process low-grade ores as accessible higher grade ores become depleted. This is against a backdrop of increasing global demands for base and precious metals, and rare earth elements. Typically about 99% of solid material hauled to, and ground at, the land surface currently ends up as waste (rock dumps and mineral tailings). Exposure of these to air and water frequently leads to the formation of acidic, metal-contaminated run-off waters, referred to as acid mine drainage, which constitutes a severe threat to the environment. Formation of acid drainage is a natural phenomenon involving various species of lithotrophic (literally rock-eating) bacteria and archaea, which oxidise reduced forms of iron and/or sulfur. However, other microorganisms that reduce inorganic sulfur compounds can essentially reverse this process. These microorganisms can be applied on industrial scale to precipitate metals from industrial mineral leachates and acid mine drainage streams, resulting in a net improvement in metal recovery, while minimising the amounts of leachable metals to the tailings storage dams. Here, we advocate that more extensive exploitation of microorganisms in metal mining operations could be an important way to green up the industry, reducing environmental risks and improving the efficiency and the economy of metal recovery.Research of AJMS and ISA was supported by European Research
Council (ERC) grant [project 323009] and Gravitation grant
[project 024.002.002] from the Netherlands Ministry of Education,
Culture and Science
Nutrients In a Circular Economy (NICE) : Bijdragen van Wageningen-UR aan de discussie over NICE
Role of nickel in high rate methanol degradation in anaerobic granular sludge bioreactors
The effect of nickel deprivation from the influent of a mesophilic (30°C) methanol fed upflow anaerobic sludge bed (UASB) reactor was investigated by coupling the reactor performance to the evolution of the Methanosarcina population of the bioreactor sludge. The reactor was operated at pH 7.0 and an organic loading rate (OLR) of 5–15 g COD l−1 day−1 for 191 days. A clear limitation of the specific methanogenic activity (SMA) on methanol due to the absence of nickel was observed after 129 days of bioreactor operation: the SMA of the sludge in medium with the complete trace metal solution except nickel amounted to 1.164 (±0.167) g CH4-COD g VSS−1 day−1 compared to 2.027 (±0.111) g CH4-COD g VSS−1 day−1 in a medium with the complete (including nickel) trace metal solution. The methanol removal efficiency during these 129 days was 99%, no volatile fatty acid (VFA) accumulation was observed and the size of the Methanosarcina population increased compared to the seed sludge. Continuation of the UASB reactor operation with the nickel limited sludge lead to incomplete methanol removal, and thus methanol accumulation in the reactor effluent from day 142 onwards. This methanol accumulation subsequently induced an increase of the acetogenic activity in the UASB reactor on day 160. On day 165, 77% of the methanol fed to the system was converted to acetate and the Methanosarcina population size had substantially decreased. Inclusion of 0.5 μM Ni (dosed as NiCl2) to the influent from day 165 onwards lead to the recovery of the methanol removal efficiency to 99% without VFA accumulation within 2 days of bioreactor operation
Redirecting nutrients in urban waste to urban agriculture
Until the nineteenth century, ‘nightsoil’ and organic waste were recycled to agriculture to replenish farmland with nutrients and organic matter in (peri-)urban areas. However, with the onset of cheap chemical fertilizer production, nightsoil use was abandoned. This development facilitated the geographic disconnection between food production and consumption, leading to the expansion of agriculture on distant soils. This chapter introduces new approaches to food production and ‘waste’ management, including the opportunity to partially close nutrient cycles on the urban scale. Moreover, this chapter provides an overview of wastewater sources, scales and systems and discusses the opportunities and constraints of recycling human excreta to urban agriculture as a means to restore the nutrient cycle in the food system. Finally, the chapter looks ahead to future research trends in this area
Immobilization of arsenic by a thermoacidophilic mixed culture with pyrite as energy source
Arsenic is associated with a wide range of ores and therefore is a major contaminant in metallurgical wastewater. For long-term immobilization of arsenic, iron arsenate as the very stable mineral scorodite (FeAsO4·2H2O) is the preferred arsenic carrier. Scorodite can be formed with the aid of microorganisms of the natural iron cycle living at pH below 2 and high temperatures (>60˚C), which carry out the oxidation of ferrous iron with oxygen. As arsenic in metallurgical waste streams is often present as arsenite, oxidation to arsenate is needed for scorodite formation. Here, we have investigated the role of a thermoacidophilic mixed culture in the oxidation of As(III) and precipitation of As(V) in the form of scorodite from a synthetic wastewater containing 6.7mM of As(III) and 0.5%wt pyrite as main iron Fe(II) source. The results indicate that As(III) was completely oxidized from the synthetic wastewater in the presence of pyrite and scorodite was formed only in presence of the mixed culture at a Fe/As:1.3. This means a combination of biological oxidation with biocrystallisation accomplished by the presence of pyrite not only as the main energy source for the microorganisms but also as catalyst in the As(III) oxidation reaction
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