Insulin deficiency exacerbates cerebral amyloidosis and behavioral deficits in an Alzheimer transgenic mouse model

<p>Abstract</p> <p>Background</p> <p>Although increasing evidence has indicated that brain insulin dysfunction is a risk factor for Alzheimer disease (AD), the underlying mechanisms by which insulin deficiency may impact the development of AD are still obscure. Using a streptozotocin (STZ)-induced insulin deficient diabetic AD transgenic mouse model, we evaluated the effect of insulin deficiency on AD-like behavior and neuropathology.</p> <p>Results</p> <p>Our data showed that administration of STZ increased the level of blood glucose and reduced the level of serum insulin, and further decreased the phosphorylation levels of insulin receptors, and increased the activities of glycogen synthase kinase-3α/β and c-Jun N-terminal kinase in the APP/PS1 mouse brain. We further showed that STZ treatment promoted the processing of amyloid-β (Aβ) precursor protein resulting in increased Aβ generation, neuritic plaque formation, and spatial memory deficits in transgenic mice.</p> <p>Conclusions</p> <p>Our present data indicate that there is a close link between insulin deficient diabetes and cerebral amyloidosis in the pathogenesis of AD.</p

Design for Motor Controller in Hybrid Electric Vehicle Based on Vector Frequency Conversion Technology

Motor and its control technology are one of the main components of Hybrid Electric Vehicle (HEV). To meet HEV's fast torque response, vector control algorithm based on rotor flux-oriented and simulation model is concerned and modular designs for controller's hardware and software are presented in the paper in order to build a platform to achieve the vector control of asynchronous induction motor. Analyze the controller's electromagnetic compatibility, introduce the corresponding antijamming measures to assure the normal operation of the electromagnetic sensitive devices such as CAN bus; experiment proves that the measure is practical and feasible. On the basis of the control logic correct, such as improving CAN bus communication reliability, assuring power-on sequence and fault treatment, carry on the motor bench experiment, test its static properties, and adjust the controller parameters. The experimental results show that the designed driving system has the performance of low speed and high torque, a wide range of variable speed and high comprehensive efficiency

IMPLEMENT AN EXTENSION CORD POWER SOCKET WITH EMERGENCY LIGHTING AND VOICE CONTROL LIGHTING FUNCTIONS

Conventional power sockets are equipped with an extension cord. Even with anti-overload devices, incidents of accidental discharge or damage to electrical appliances may still incur due to external factors. To address this problem, this study integrated the voice control and lighting functions to the design of the power socket with an extension cord. The proposed design is space-efficient and versatile, and can be used in living rooms, bedrooms, and other places having electrical appliances. The proposed design also integrated emergency lighting and flashlight functions, so as to expand the practicality and safety of the power socket

Label-free biochips for rapid detection of soybean allergen GlymBd 30K (P34) in foods

Purpose: To develop an innovative method for detection of soybean allergen, Gly mBd 30K (P34) in foods using a biosensor based on high spatial imaging ellipsometer.Methods: Two monoclonal antibodies, 2D1 and 5F9, each known to have specific bioactivity against P34 allergen, were selected and separately immobilized as ligands on silicon wafer surface to allow capture of the P34 allergen. The resultant changes on the wafer surface were viewed directly as images in gray scale.Results: Images indicated that these two antibodies detected the presence of P34 allergen in soybean extract with sensitivity of 1 mg/L and a detection time of about 15 min. For the detection of P34 allergen in foods, results from biochip detection were consistent with those obtained using ELISA detection.Conclusion: These results show that the biochip may be an effective analytical tool for food allergen detection.Keywords: Soybean Allergen, Gly mBd 30K, Biochip, Detection, Food

Vertically Aligned Graphene Prepared by Photonic Annealing for Ultrasensitive Biosensors

Graphene exhibits excellent physical, electronic, and chemical properties that are highly desirable for biosensing applications. However, most graphene biosensors are based on graphene lying flat on a substrate and therefore do not utilize its maximum specific surface area for ultrasensitive detection. Herein, we report the novel use of photonic annealing on a flexographically printed graphene–ethyl cellulose composite to produce vertically aligned graphene (VAG) biosensors for ultrasensitive detection of algal toxins in drinking water. These VAG structures, which maximized the specific surface area of graphene, were formed by partial removal of the polymeric binder upon applying intense pulsed light on the printed graphene. A label-free and low-cost VAG biosensor based on a non-faradaic electrochemical impedance spectroscopy technique was fabricated. The biosensor exhibited a limit of detection of 1.2 ng/L for microcystin-LR in local tap water. Such an ultrasensitive VAG biosensor is suitable for low-cost mass production using an integrated roll-to-roll flexographic printing with rapid photonic annealing technique