Multi-wavelength Stellar Polarimetry of the Filamentary Cloud IC5146: I. Dust Properties

We present optical and near-infrared stellar polarization observations toward the dark filamentary clouds associated with IC5146. The data allow us to investigate the dust properties (this paper) and the magnetic field structure (Paper II). A total of 2022 background stars were detected in $R_{c}$-, $i'$-, $H$-, and/or $K$-bands to $A_V \lesssim 25$ mag. The ratio of the polarization percentage at different wavelengths provides an estimate of $\lambda_{max}$, the wavelength of peak polarization, which is an indicator of the small-size cutoff of the grain size distribution. The grain size distribution seems to significantly change at $A_V \sim$ 3 mag, where both the average and dispersion of $P_{R_c}/P_{H}$ decrease. In addition, we found $\lambda_{max}$ $\sim$ 0.6-0.9 $\mu$m for $A_V>2.5$ mag, which is larger than the $\sim$ 0.55 $\mu$m in the general ISM, suggesting that grain growth has already started in low $A_V$ regions. Our data also reveal that polarization efficiency (PE $\equiv P_{\lambda}/A_V$) decreases with $A_V$ as a power-law in $R_c$-, $i'$-, and $K$-bands with indices of -0.71$\pm$0.10, -1.23$\pm$0.10 and -0.53$\pm$0.09. However, $H$-band data show a power index change; the PE varies with $A_V$ steeply (index of -0.95$\pm$0.30) when $A_V < 2.88\pm0.67$ mag but softly (index of -0.25$\pm$0.06) for greater $A_V$ values. The soft decay of PE in high $A_V$ regions is consistent with the Radiative Aligned Torque model, suggesting that our data trace the magnetic field to $A_V \sim 20$ mag. Furthermore, the breakpoint found in $H$-band is similar to the $A_V$ where we found the $P_{R_c}/P_{H}$ dispersion significantly decreased. Therefore, the flat PE-$A_V$ in high $A_V$ regions implies that the power index changes result from additional grain growth.Comment: 31 pages, 17 figures, and 3 tables; accepted for publication in Ap

Antioxidant Properties and Anti-inflammatory Effects of the Hydroethanolic Extracts of Two Varieties of Bayberry fruit (Myrica rubra Sieb et Zucc.) Prepared by Stirring and Ultrasonic Methods

Chinese bayberry (Myrica rubra Sieb. et Zucc.) is an economically important medicinal plant with multiple uses. Two varieties ‘Dongkui Oriental Pearl’ (Dongkui for short) and acuminata ‘Nakai’ (Nakai for short) were used to compare and evaluate the antioxidant activities of hydroethanolic extracts of the fruit using ultrasonic and stirring extraction methods. Dongkui bayberry fruit extract (BFE) prepared using the ultrasonic method exhibited a significantly higher value for the total phenolic content (TPC) and had lower 50% inhibitory concentration (IC50) values of scavenging activities against 1,1-diphenyl-2- picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and hydrogen peroxide (H2O2), as well as reducing power compared to the other treatment. The TPC of the BFE was significantly correlated with its DPPH, ABTS, and H2O2 radical-scavenging and reducing power activities. Dongkui BFE at a concentration of 0.25 mg/mL exhibited significantly greater protection of RAW 264.7 mouse macrophages against H2O2-induced damage and lipopolysaccharide (LPS)-stimulated nitric oxide production by macrophages, and it displayed remarkable inhibitory effects compared to the other extracts using the ultrasonic extraction method. Furthermore, compared to the Nakai BFE, macrophages exposed to the Dongkui BFE by the ultrasonic extraction method significantly inhibited LPS-induced production of tumor necrosis factor-α at a concentration of the extract of 0.25 mg/mL. The antioxidant properties and anti-inflammatory and protective effects of BFE prepared by stirring and ultrasonic methods are discussed for the first time in this study

Light Wave Propagation and Scattering Through Particles

The study of light propagating and scattering for various particles has always been important in many practical applications, such as optical diagnostics for combustion, monitoring of atmospheric pollution, analysis of the structure and pathological changes of the biological cell, laser Doppler technology, and so on. This chapter discusses propagation and scattering through particles. The description of the solution methods, numerical results, and potential application of the light scattering by typical particles is introduced. The generalized Lorenz-Mie theory (GLMT) for solving the problem of Gaussian laser beam scattering by typical particles with regular shapes, including spherical particles, spheroidal particles, and cylindrical particles, is described. The numerical methods for the scattering of Gaussian laser beam by complex particles with arbitrarily shape and structure, as well as random discrete particles are introduced. The essential formulations of numerical methods are outlined, and the numerical results for some complex particles are also presented

Modification of 1D TiO2 nanowires with GaOxNy by atomic layer deposition for TiO2@GaOxNy core-shell nanowires with enhanced photoelectrochemical performance

As a well-known semiconductor that can catalyse the oxygen evolution reaction, TiO2 has been extensively investigated for its solar photoelectrochemical water properties. Unmodified TiO2 shows some issues, particularly with respect to its photoelectrochemical performance. In this paper, we present a strategy for the controlled deposition of controlled amounts of GaOxNy cocatalysts on TiO2 1D nanowires (TiO2@GaOxNy core-shell) using atomic layer deposition. We show that this modification significantly enhances the photoelectrochemical performance compared to pure TiO2 NW photoanodes. For our most active TiO2@GaOxNy core-shell nanowires with a GaOxNy thickness of 20 nm, a photocurrent density up to 1.10 mA cm-2 (at 1.23 V vs RHE) under AM 1.5 G irradiation (100 mW cm-2) has been achieved, which is 14 times higher than that of unmodified TiO2 NWs. Furthermore, the band gap matching with TiO2 enhances absorption of visible light over unmodified TiO2 and the facile oxygen vacancy formation after deposition of GaOxNy also provides active sites for water activation. Density functional theory studies of model systems of GaOxNy-modified TiO2 confirm the band gap reduction, high reducibility and ability to activate water. The highly efficient and stable systems of TiO2@GaOxNy core-shell nanowires with ALD deposited GaOxNy demonstrates a good strategy for fabrication of core-shell structures that enhances the photoelectrochemical performance of readily available photoanodes

A novel PCR strategy for high-efficiency, automated site-directed mutagenesis

We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flanking primers and one mutagenic primer with different melting temperatures (T(m)) are used together in a single PCR tube continuously without any intervention. A single-stranded mutagenic DNA (smDNA) is synthesized utilizing the high T(m) mutagenic primer at a high annealing temperature, which prevents the priming of the low T(m) primers (i.e. the two flanking primers). A megaprimer is then produced using this smDNA as the template at a denaturing temperature that prevents wild-type template DNA activity. The desired mutant DNA is then obtained by cycling again through these first two steps, resulting in a mutagenic efficiency of 100% in all tested cases. This highly automated method not only eliminates the necessity of any intermediate manipulation and accomplishes the mutagenesis process in a single round of PCR but, most notably, enables complete success of mutagenesis. This novel method is also both cost and time efficient and fully automated