Evolution mechanism of microstructure and microhardness of Ti–6Al–4V alloy during ultrasonic elliptical vibration assisted ultra-precise cutting

The ultra-precision Ti–6Al–4V alloy parts are growing used in medical and aerospace industries, and which always work in the extreme working conditions such as high temperature, high pressure, and variable load. Thus, the requirements for machining accuracy and surface quality of parts are getting higher and higher. The ultrasonic elliptical vibration assisted cutting (UEVC) technology has been proved to be an effective method for the ultra-precision machining of Ti–6Al–4V alloy. However, in the UEVC process, the evolution mechanism of microstructure and microhardness, which directly affect the service performance and life, is unrevealed. In this paper, the comprehensive investigations of microstructural plastic deformation, grain refinement, phase transformation and microhardness of machined surface layer under conventional cutting (CC) and UEVC processes are carried out. The experimental results indicated that, due to the effects of UEVC technology, the plastic deformation area show obvious compression deformation, the depth of plastic deformation is less than 10 μm, there is no obvious phase transformation on the machined surface layer material, and the hardening rate of machined surface is more than 20%. These findings show the UEVC technology has a unique influence on the microstructure and microhardness of Ti–6Al–4V alloy, which have important implications for the cutting parameter design of ultra-precision Ti–6Al–4V alloy parts

HIV-1 Membrane-Proximal External Region Fused to Diphtheria Toxin Domain-A Elicits 4E10-Like Antibodies in Mice.

The production of broadly neutralizing antibodies (bNAbs) is a major goal in the development of an HIV-1 vaccine. The membrane-proximal external region (MPER) of gp41, which plays a critical role in the virus membrane fusion process, is highly conserved and targeted by bNAbs 2F5, 4E10, and 10E8. As such, MPER could be a promising epitope for vaccine design. In this study, diphtheria toxin domain A (CRM197, amino acids 1-191) was used as a scaffold to display the 2F5 and 4E10 epitopes of MPER, named CRM197-A-2F5 and CRM197-A-4E10. Modest neutralizing activities were detected against HIV-1 clade B and D viruses in the sera from mice immunized with CRM197-A-4E10. Monoclonal antibodies raised from CRM197-A-4E10 could neutralize several HIV-1 strains, and epitope-mapping analysis indicated that some antibodies recognized the same amino acids as 4E10. Collectively, we show that 4E10-like antibodies can be induced by displaying MPER epitopes using an appropriate scaffold. These results provide insights for HIV-1 MPER-based immunogens design

Neutralizing human anti-B-cell-activating factor of the TNF family (BAFF) scFv selected from phage antibody library

Abstract Elevated levels of B-cell-activating factor of the TNF family (BAFF) have been implicated in the pathogenesis of autoimmune diseases in human. We now report the isolation by phage display of human single-chain antibody fragment (scFv) anti-BAFF. After four rounds of panning against BAFF, thirty-two out of 92 phage clones displayed BAFF binding activity. One of the positive clones, designated F8, bound to BAFF with relatively high affinity and neutralized BAFF bioactivity in vitro. F8 clone was expressed in soluble form in Escherichia coli HB2151 and purified by immobilized metal affinity chromatography (IMAC). The purified scFv recognized BAFF with the affinity constant (K aff ) of 2.5 × 10 7 M −1 without cross-reaction to APRIL. In addition to binding, the purified scFv could does-dependently inhibit BAFF-induced mouse spleen B lymphocyte proliferation. Together with its fully human mature, F8 scFv may have therapeutic implications in therapy of autoimmune disorders mediated by BAFF

Automatic seismic event tracking using a dynamic time warping algorithm

For seismic data interpretation, horizon picking based on seismic events in stacked or migrated seismic sections is essential for obtaining information on subsurface structures. This conventional work is time-consuming via manual implementation. In this paper, we develop an automatic seismic event tracking method of horizon interpretation using the dynamic time warping (DTW) algorithm. The proposed method consists of two steps: calculating local time shifts between adjacent traces through a pilot trace and then event tracking. In the method, the DTW algorithm is applied to calculate time shifts between two adjacent traces, and an improved multitrace DTW strategy is proposed to improve the robustness. One synthetic seismic trace is used to demonstrate the DTW algorithm, and a synthetic seismic section is used to verify the feasibility of the proposed method handling contaminated seismic data with noise. Finally, we apply the method to a 3D physical model dataset. The result indicates that the proposed method is quantitatively feasible for seismic event automatic tracking and is reasonably stable for noisy seismic section flattening, which also has the potential to extract seismic horizon slices effectively

Chemical Compositional Analysis of Catalytic Hydroconversion Products of Heishan Coal Liquefaction Residue

Liquefaction residue of Heishan bituminous coal (HLR) was subject to two hydroconversion reactions under 5 MPa initial pressure of hydrogen at 300°C for 3 h, without catalyst and with acid supported catalyst (ASC), respectively. The reaction products were analyzed with gas chromatography/mass spectrometer (GC/MS). The results show that 222 organic compounds were detected totally in the products and they can be divided into alkanes, aromatic hydrocarbons (AHCs), phenols, ketones, ethers, and other species (OSs). The yield of hydroconversion over the ASC is much higher than that without catalyst. The most abundant products are aromatic hydrocarbons in the reaction products from both catalytic and noncatalytic reactions of HLR. The yield of aromatic hydrocarbons in the reaction product from hydroconversion with the ACS is considerably higher than that from hydroconversion without a catalyst

Synergistic effect of thermal oxygen and UV aging on natural rubber

The oxidation of natural rubber (NR) leads to a decrease in mechanical properties, even resulting in failure of NR products. Many studies focusing on this single factor have failed to fully elucidate the impact of tropical island environment on NR properties. Based on this concern, the synergistic effect of thermal oxygen and ultraviolet (UV) aging on NR was systematically studied. The results revealed that thermal oxygenation can promote UV aging, which leads to the appearance of surface cracks and deepening of color. With the extension of aging time, the mechanical properties of NR correspondingly decreased. Besides, to deeply understand the mechanism of the synergistic effect of thermal oxygen and UV aging on NR, we selected squalene to simulate and analyze the molecular structure changes in NR. Based on these results, a possible synergistic effect of thermal oxygen and UV aging mechanisms on NR could be proposed

MXene Enabling the Long-Term Superior Thermo-Oxidative Resistance for Elastomers

The ability of long-term thermo-oxidative resistance is very important for elastomers in application. However, many conventional antioxidants are difficult to realize the long-term thermo-oxidative resistance. To overcome this limitation, a design strategy is introduced by combing elastomers with MXene and natural rubber (NR) is chosen as a model material. MXene is efficient in absorbing oxygen and the generated free radicals in the NR matrix and can inhibit the diffusion of oxygen toward the interior. Moreover, MXene, like graphene and carbon black, absorbs molecular chains, inhibiting the migration of MXene toward the surface of the sample. Such characteristics of MXene endow NR/MXene with the long-term outstanding thermo-oxidative resistance. For example, after three days of the thermo-oxidative process for NR/MXene, the tensile strength is 19 MPa and the retention of tensile strength is 63%, which far exceeds the effects of conventional antioxidants. This work not only provides a good guide for the universal design of elastomers with long-term thermo-oxidative resistance but also expands the application of MXene