The Analgesic Activity of Bestatin as a Potent APN Inhibitor

Bestatin, a small molecular weight dipeptide, is a potent inhibitor of various aminopeptidases as well as LTA4 hydrolase. Various physiological functions of Bestatin have been identified, viz.: (1) an immunomodifier for enhancing the proliferation of normal human bone marrow granulocyte–macrophage progenitor cells to form CFU-GM colonies; Bestatin exerts a direct stimulating effect on lymphocytes via its fixation on the cell surface and an indirect effect on monocytes via aminopeptidase B inhibition of tuftsin catabolism; (2) an immunorestorator and curative or preventive agent for spontaneous tumor; Bestatin alone or its combination with chemicals can prolongate the disease-free interval and survival period in adult acute or chronic leukemia, therefore, it was primarily marketed in 1987 in Japan as an anticancer drug and servers as the only marketed inhibitor of Aminopeptidase N (APN/CD13) to cure leukemia to date; (3) a pan-hematopoietic stimulator and restorator; Bestatin promotes granulocytopoiesis and thrombocytopoiesis in vitro and restores them in myelo-hypoplastic men; (4) an inhibitor of several natural opioid peptides. Based on the knowledge that APN can cleave several bioactive neuropeptides such as Met-enkaphalins, Leu-enkaphalins, β-Endorphin, and so on, the anti-aminopeptidase action of Bestatin also allows it to protect endopeptides against their catabolism, exhibiting analgesic activity. Although many scientific studies and great accomplishments have been achieved in this field, a large amount of problems are unsolved. This article reviews the promising results obtained for future development of the analgesic activity of Bestatin that can be of vital interest in a number of severe and chronic pain syndromes

EFFECT OF WEARABLE FITNESS WATCH ON PHYSICAL ACTIVITY IN SCHOOL CHILDREN WITH OVERWEIGHT

Overweight and obesity in children and adolescents is a significant health problem, and lack of physical activity may be a factor related to obesity. In this study, we explored the wearable fitness watch to monitor the physical activity promoted situation of overweight and obese schoolchildren. Participants were overweight schoolchildren divided into two groups, the study group (n=41) and the control group (n=44). In the control group, the wearable fitness watch only displays the time. In the study group, each week, participants set up the goal, which increases the steps by 10% from baseline steps to reach 10,000 steps. The ANOVA is adopted to analyze the parameters such as the average daily number of steps, moderate to vigorous physical activity (MVPA). The results indicated the study group increase and improved daily walking steps and MVPA accumulation time in 8 weeks. The wearable fitness watch can help to promote the physical activity of overweight children

Natures of Tcs(2900)T_{cs}(2900) and Tcsˉa(2900)T^a_{c\bar{s}}(2900)

Inspired by the states $T_{cs0}(2900)^0$, $T_{cs1}(2900)^0$, $T^a_{c\bar{s}0}(2900)^{0}$ and $T^a_{c\bar{s}0}(2900)^{++}$ reported by the LHCb Collaboration, we carry out a systematical investigation on the properties of the ground and $P$-wave states $[cs][\bar{u}\bar{d}]$ and $[cu][\bar{s}\bar{d}]$ with various spin, isospin or $U$-spin, and color combinations in a multiquark color flux-tube model. Matching our results with the spin-parity and mass of the states $T_{cs0}(2900)^0$ and $T_{cs1}(2900)^0$, we can describe them as the compact states $[cs][\bar{u}\bar{d}]$ with $I(J^{P})=1(0^+)$ and $0(1^-)$ in the model, respectively. The ground state $T_{cs0}(2900)^0$ is mainly made of strongly overlapped axial-vector $[cs]_{\bar{\mathbf{3}}_c}$ and axial-vector $[\bar{u}\bar{d}]_{\mathbf{3}_c}$. The $P$-wave state $T_{cs1}(2900)^0$ is dominantly consisted of gradually separated scalar or axial vector $[cs]_{\bar{\mathbf{3}}_c}$ and scalar $[\bar{u}\bar{d}]_{\mathbf{3}_c}$ in the shape of a dumbbell. Supposing the states $T^a_{c\bar{s}0}(2900)^{0}$ and $T^a_{c\bar{s}0}(2900)^{++}$ belong to the same isospin triplet, the mass of the state $\left [[cu]_{\bar{\mathbf{3}}_c}[\bar{s}\bar{d}]_ {\mathbf{3}_c}\right ]_{\mathbf{1}_c}$ with symmetrical $U$-spin and $J^P=0^+$ is highly consistent with that of the states $T^a_{c\bar{s}0}(2900)^{0}$ and $T^a_{c\bar{s}0}(2900)^{++}$ in the model. After coupling two color configurations, the state $[cu][\bar{s}\bar{d}]$ is a little lighter than the states $T^a_{c\bar{s}0}(2900)^{0}$ and $T^a_{c\bar{s}0}(2900)^{++}$. In addition, we also discuss the properties of other states in the model.Comment: 8 pages, 4 tables, comments are welcom

Imaging features of aggressive fibromatosis in psoas muscle

Aggressive fibromatosis (AF) of psoas muscle origin is extremely rare and little is known about its radiological features. We here present such a case in a 24-year-old man with psoas AF and ilium bone involvement. The authors stress the contibutive diagnostic role of MRI

Oxalate-bridged heterometallic chains with monocationic dabco derivatives

A series of bimetallic oxalate-bridged one-dimensional chains with monocationic dabco derivatives, ({R-dabco}[M(solv)2][Cr(ox)3]·n(solv)) (dabco = 1,4-diazabicyclo[2.2.2]octane, H2ox = oxalate; R = H, M = Co (1); R = H, M = Zn (2); R = Bu, M = Co (3); R = Bu, M = Zn (4)) were synthesized. All compounds have one-dimensional zig-zag chain structures with R-dabco cations located between chains. Cryomagnetic studies reveal that 1 and 3 showed intrachain ferromagnetic interactions between Co(II) and Cr(III) ions and metamagnetic behaviour due to interchain antiferromagnetic interactions. Permittivity measurements on compound 4 indicate specific paraelectronic relaxation behaviour originating from the rotational motion of the dabco alkyl substituent

A numerical study of parachute inflation based on a mixed method

ἀ e C9 parachute was the research object in this work and was studied by using a fluid-structure interaction method and CFD method. An arbitrary Lagrangian-Eulerian method, a kind of fluid-structure interaction method, was used to simulate the inflation process. ἀ e dynamic relationship between canopy shape and flow field was obtained. ἀ e canopy shape in a stable phase was exported and was transformed into the porous media domain. ἀ en the flow around the canopy shape was simulated by the CFD method we used based on the k-ε turbulence model. ἀ e experiments verified the accuracy of structural change and the feasibility of the porous media model. ἀ e arbitrary Lagrangian-Eulerian method not only can obtain the dynamic results of structure and flow field but also can provide a more accurate bluff body for further CFD analysis. ἀ e CFD method based on porous media and the turbulence model can obtain more detailed and accurate flow field results, which can be used as a complement to fluid-structure interaction analysis. ἀi s mixed method can improve the accuracy of analysis and be useful for other permeable fabric research

Effect of compounds on the purification and antibody preparation of the extracellular domain fragment of the receptor CD163

<p>Abstract</p> <p>Background</p> <p>Porcine reproductive and respiratory syndrome virus (PRRSV) has been acknowledged as one of the most important agents affecting swine. The scavenger receptor CD163 is one of the important entry mediators for PRRSV.</p> <p>Results</p> <p>The tD4 and tD5 CD163 genes were amplified, and the PCR products were cloned into pET-28a(+) (designated pET-28a-tD4 and pET-28a-tD5, respectively). The plasmids pET-28a-tD4 and pET-28a-tD5 were then transformed into the <it>E. coli </it>BL21 (DE3) strain and expressed by adding 1 mmol/L of isopropyl-beta-D-thiogalactopyranoside. The proteins were highly expressed in the supernatant from the tD4- and tD5-producing cells that were incubated with a binding buffer containing the following compounds: β-mercaptoethanol, urea, Tween 20, glycerol, and SDS, while they were rarely expressed in the supernatant from the tD4- and tD5-producing cells that were incubated with binding buffer without the compounds. The tD4 and tD5 proteins were purified, and BALB/c mice were immunized with the purified proteins. Western blotting analysis showed that the tD4 and tD5 proteins were capable of reacting with tD5 antibodies; the titer of both the tD4 and tD5 antiserums was 1:160 against the tD5 protein, as shown by ELISA.</p> <p>Conclusions</p> <p>These studies provide a new way for the purification of proteins expressed in inclusion bodies and the preparation of the corresponding antibodies.</p