Proteomic characterization of novel protein-protein interactions for understanding functions of gene products

Ph.DDOCTOR OF PHILOSOPH

Equine herpesvirus type 1 infection induces procoagulant activity in equine monocytes

The alphaherpesvirus, equine herpesvirus type 1 (EHV-1), is a highly prevalent cause of equine infectious abortion and encephalomyelopathy. These syndromes have been attributed to ischemic necrosis from thrombosis in placental and neural vessels, although the mechanisms underlying thrombosis are unknown. After inhalation, EHV-1 establishes a peripheral blood mononuclear cell- associated viremia, with monocytes being a target of infection. Monocytes are also the main source of tissue factor (TF) in diseased states. Since TF is the primary activator of coagulation, increased monocyte TF expression could be involved in EHV-1-associated thrombosis. We hypothesized that EHV-1 infection would induce TF-dependent procoagulant activity in equine monocytes. Monocyte- enriched fractions of blood were infected with abortigenic (RacL11, NY03) and neuropathogenic (Ab4) EHV-1 strains. All strains induced procoagulant activity, to variable degrees, within 1 to 4 h, with maximal activity at 24 h, after infection. Virus-induced procoagulant activity was similar to that seen with lipopolysaccharide, a known stimulant of TF-mediated procoagulant responses. Virus-induced procoagulant activity was factor VIIa-dependent and temporally associated with TF gene transcription, implicating TF as the main driver of the activity. Procoagulant activity was mildly decreased (30-40%) when virus was inactivated by ultraviolet light or when infected cells were treated with aphidicolin, a virus DNA polymerase inhibitor, suggesting early events of virus infection (attachment, entry or intracellular trafficking) are the primary stimulus of procoagulant activity. Our results indicate that EHV-1 rapidly stimulates procoagulant activity in equine monocytes in vitro. The EHV-1-induced procoagulant activity in monocytes may contribute to clinical thrombosis in horses with EHV-1 infection

On Mx / G(M/H)/1 Retrial System with Vacation: Service Helpline Performance Measurement

This paper analyzes an unreliable MX/G(M/H)/1MX/G(M/H)/1 retrial system with vacation. We present closed-form expressions for the important performance indicators of the system, and derive the optimal vacation policies for minimizing the average waiting time of orbiting customers. The performance metrics relevant for helpline services are developed. Numerical experiments are conducted to examine the effect of vacation policy on the queue length and busy period of the system

Preparation and characterization of tin oxide, SnO2 nanoparticles decorated graphene

SnO2 nanoparticles/graphene (SnO2/GP) nanocomposite was synthesized by a facile microwave method. The X-ray diffraction (XRD) pattern of the nanocomposite corresponded to the diffraction peak typical of graphene and the rutile phase of SnO2 with tetragonal structure. The field emission scanning electron microscope (FESEM) images revealed that the graphene sheets were dotted with SnO2 nanoparticles with an average size of 10 nm. The X-ray photoelectron spectroscopy (XPS) analysis indicated that the development of SnO2/GP resulted from the removal of the oxygenous groups on graphene oxide (GO) by Sn2+ ions. The nanocomposite modified glassy carbon electrode (GCE) showed excellent enhancement of electrochemical performance when interacting with mercury(II) ions in potassium chloride supporting electrolyte. The current was increased by more than tenfold, suggesting its potential to be used as a mercury(II) sensor

MALAY ANIMISM AND ISLAMIC ORTHODOXY IN SINGAPORE

Bachelor'sBACHELOR OF SOCIAL SCIENCES (HONOURS

Optimal warranty policies for systems with imperfect repair

We investigate a system whose basic warranty coverage is minimal repair up to a specified warranty length. An additional service is offered whereby first failure is restored up to the consumers’ chosen level of repair. The problem is studied under two system replacement strategies: periodic maintenance before and after warranty. It turns out that our model generalizes the model of Rinsaka and Sandoh [K. Rinsaka, H. Sandoh, A stochastic model with an additional warranty contract, Computers and Mathematics with Applications 51 (2006) 179–188] and the model of Yeh et al. [R.H. Yeh, M.Y. Chen, C.Y. Lin, Optimal periodic replacement policy for repairable products under free-repair warranty, European Journal of Operational Research 176 (2007) 1678–1686]. We derive the optimal maintenance period and optimal level of repair based on the structures of the cost function and failure rate function. We show that under certain assumptions, the optimal repair level for additional service is an increasing function of the replacement time. We provide numerical studies to verify some of our results

Optimal inventory policy with supply uncertainty and demand cancellation

We consider a periodic review model where the firm manages its inventory under supply uncertainty and demand cancellation. We show that because of supply uncertainty, the optimal inventory policy has the structure of re-order point type. That is, we order if the initial inventory falls below this re-order point, otherwise we do not order. This is in contrast to the work of Yuan and Cheung (2003) who prove the optimality of an order up to policy in the absence of supply uncertainty. We also investigate the impact of supply uncertainty and demand cancellation on the performance of the supply chain. Using our model, we are able to quantify the importance of reducing the variance of either the distribution of yield or the distribution of demand cancellation. The single, multiple periods and the infinite horizon models are studied

Impact of transportation contract on inventory systems with demand cancellation

Supply contracts often specify the quantity of inventory for shipments where retailers are liable to pay for ordering costs if order quantity exceeds the contracted size. We analyze a periodic review system where the firm manages its demand that are reserved with a one- period leadtime together with a multi-tier supply contract. We show that the optimal inventory policy has the primary structure of “finite generalized base stock” policy whose critical numbers depend on reservation parameters. The single, multiple periods and the infinite horizon models are studied. The presence of ordering costs needs a different approach from that in Yuan and Cheung (2003) to analyze the infinite horizon model